The DOI matches its Crossref record; 79 of the 79 references it cites were found in a registry or an index.
Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches
Abstract
The cryopreservation of spermatozoa was introduced in the 1960s as a route to fertility preservation. Despite the extensive progress that has been made in this field, the biological and biochemical mechanisms involved in cryopreservation have not been thoroughly elucidated to date. Various factors during the freezing process, including sudden temperature changes, ice formation and osmotic stress, have been proposed as reasons for poor sperm quality post-thaw. Little is known regarding the new aspects of sperm cryobiology, such as epigenetic and proteomic modulation of sperm and trans-generational effects of sperm freezing. This article reviews recent reports on molecular and cellular modifications of spermatozoa during cryopreservation in order to collate the existing understanding in this field. The aim is to discuss current freezing techniques and novel strategies that have been developed for sperm protection against cryo-damage, as well as evaluating the probable effects of sperm freezing on offspring health.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures
POLGE, C., SMITH, A. U., & PARKES, A. S. (1949) · Nature · doi:10.1038/164666a0 · Cited by 2,081
The effects of cryopreservation on sperm morphology, motility and mitochondrial function
O'Connell, M., McClure, N., & Lewis, S. E. M. (2002) · Human Reproduction · doi:10.1093/humrep/17.3.704 · Cited by 510
Effects of cryopreservation procedures on sperm membranes
Parks, J. E., & Graham, J. K. (1992) · Theriogenology · doi:10.1016/0093-691x(92)90231-f · Cited by 430
Yeste, M. (2016) · Theriogenology · doi:10.1016/j.theriogenology.2015.09.047 · Cited by 391
The presence, role and clinical use of spermatozoal RNAs
Jodar, M., Selvaraju, S., Sendler, E., Diamond, M. P., Krawetz, S. A., & for the Reproductive Medicine Network (2013) · Human Reproduction Update · doi:10.1093/humupd/dmt031 · Cited by 368
Di Santo, M., Tarozzi, N., Nadalini, M., & Borini, A. (2012) · Advances in Urology · doi:10.1155/2012/854837 · Cited by 344
The role of antioxidants in sperm freezing: a review
Amidi, F., Pazhohan, A., Shabani Nashtaei, M., Khodarahmian, M., & Nekoonam, S. (2016) · Cell and Tissue Banking · doi:10.1007/s10561-016-9566-5 · Cited by 328
Kopeika, J., Thornhill, A., & Khalaf, Y. (2015) · Human Reproduction Update · doi:10.1093/humupd/dmu063 · Cited by 326
Fertility Preservation in Adolescents and Young Adults With Cancer
Levine, J., Canada, A., & Stern, C. J. (2010) · Journal of Clinical Oncology · doi:10.1200/jco.2009.22.8312 · Cited by 323
Male infertility in cancer patients: Review of the literature
Dohle, G. R. (2010) · International Journal of Urology · doi:10.1111/j.1442-2042.2010.02484.x · Cited by 279
Metadata
- Authors
- Hezavehei, M., Sharafi, M., Kouchesfahani, H. M., Henkel, R., Agarwal, A., Esmaeili, V., & Shahverdi, A.
- Venue
- Reproductive BioMedicine Online
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 37
- Issue
- 3
- Pages
- 327-339
- ISSN
- 1472-6483 (ISSN-L)
- OpenAlex
- W2888089578
- Cited by
- 411
- References
- 79
- FWCI
- 31.66
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. https://app.cochat.ai/papers/10.1016/j.rbmo.2018.05.012
- The paper
- Hezavehei, M., Sharafi, M., Kouchesfahani, H. M., Henkel, R., Agarwal, A., Esmaeili, V., & Shahverdi, A. (2018). Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reproductive BioMedicine Online. https://doi.org/10.1016/j.rbmo.2018.05.012
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
