Novel hybrid three-dimensional artificial liver using human induced pluripotent stem cells and a rat decellularized liver scaffold
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Introduction Liver transplantation is currently the only curative therapy for end-stage liver failure; however, establishment of alternative treatments is required owing to the serious donor organ shortage. Here, we propose a novel model of hybrid three-dimensional artificial livers using both human induced pluripotent stem cells (hiPSCs) and a rat decellularized liver serving as a scaffold. Methods Rat liver harvesting and decellularization were performed as reported in our previous studies. The decellularized liver scaffold was recellularized with hiPSC-derived hepatocyte-like cells (hiPSC-HLCs) through the biliary duct. The recellularized liver graft was continuously perfused with the culture medium using a pump at a flow rate of 0.5 mL/min in a standard CO 2 (5%) cell incubator at 37 °C. Results After 48 h of continuous perfusion culture, the hiPSC-HLCs of the recellularized liver distributed into the parenchymal space. Furthermore, the recellularized liver expressed the albumin ( ALB ) and CYP3A4 genes, and secreted human ALB into the culture medium. Conclusion Novel hybrid artificial livers using hiPSCs and rat decellularized liver scaffolds were successfully generated, which possessed human hepatic functions.
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The repopulating cells secreted albumin and stained positive for albumin, AFP, and CYP3A4 after 48 h of media perfusion.
“The repopulating cells secreted albumin and stained positive for albumin, AFP, and CYP3A4 after 48 h of media perfusion.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [214]
The expression of AFP suggested insufficient maturation of the engrafted cells.
“The expression of AFP suggested insufficient maturation of the engrafted cells.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [214]
Minami et al. recellularized a rat liver scaffold with hepatocyte-like cells derived from human iPSCs via the biliary duct.
“Similarly, Minami et al. recellularized a rat liver scaffold with hepatocyte-like cells derived from human iPSCs (hiPSCs) via the biliary duct [214].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [214]
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Self-citation
- Authors
- Minami, T., Ishii, T., Yasuchika, K., Fukumitsu, K., Ogiso, S., Miyauchi, Y., Kojima, H., Kawai, T., Yamaoka, R., Oshima, Y., Kawamoto, H., Kotaka, M., Yasuda, K., Osafune, K., & Uemoto, S.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Ishii, Takamichi
- ORCID
- Venue
- Regenerative Therapy
- Publisher
- Elsevier BV
- Published
- 2019-06
- Volume
- 10
- Pages
- 127-133
- ISSN
- 2352-3204 (ISSN-L)
- PMC
- PMC6477477
- OpenAlex
- W2928427698
- Cited by
- 44
- References
- 20
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 5 · 2023: 7 · 2022: 11 · 2021: 12 · 2020: 6
- FWCI
- 6.93
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Regenerative Therapy (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion, cc-by-nc-nd)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion)
- Funders
- Japan Society for the Promotion of Science (16H05489)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- artificial liver, induced pluripotent stem cells, decellularized liver scaffold, rat liver, hepatocyte-like cells, recellularization, end-stage liver failure, organ shortage, albumin secretion
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