Fibrinogen/poly(l-lactide-co-caprolactone) copolymer scaffold: A potent adhesive material for urethral tissue regeneration in urethral injury treatment
Open accessAbstract
Since a scarcity of sufficient grafting materials, several complications can arise after urothelial defect reconstruction surgery, including severe hypospadias. Accordingly, developing alternative therapies, such as urethral restoration via tissue engineering are needed. In the present study, we developed a potent adhesive and repairing material using fibrinogen-poly(l-lactide-co-caprolactone) copolymer (Fib-PLCL) nanofiber scaffold to achieve effective urethral tissue regeneration after seeding with epithelial cells on the surface. The in vitro result found the Fib-PLCL scaffold promoted the attachment and viability of epithelial cells on their surface. The increased expression levels of cytokeratin and actin filaments were observed in Fib-PLCL scaffold than PLCL scaffold. The in vivo urethral injury repairing potential of Fib-PLCL scaffold was evaluated using a rabbit urethral replacement model. In this study, a urethral defect was surgically excised and replaced with the Fib-PLCL and PLCL scaffolds or autograft. As expected, the animals healed well after surgery in the Fib-PLCL scaffold group, and no significant strictures were identified. As expected, the cellularized Fib/PLCL grafts have induced the luminal epithelialization, urethral smooth muscle cell remodelling, and capillary development all at the same time. Histological analysis revealed that the urothelial integrity in the Fib-PLCL group had progressed to that of a normal urothelium, with enhanced urethral tissue development. Based on the results, the present study suggests that the prepared fibrinogen–PLCL scaffold is more appropriate for urethral defect reconstruction.
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Fibrinogen PLCL composite scaffold promotes adhesion and growth of epithelial cells and promotes repair and reconstruction of urethra in rabbit urethral defect model.
“The results showed that the fibrinogen PLCL composite scaffold can promote the adhesion and growth of epithelial cells, and promote the repair and reconstruction of urethra in rabbit urethral defect model [210].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [210]
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- Authors
- Jiao, W., Yu, W., Wang, Y., Zhang, J., Wang, Y., He, H., & Shi, G.
- Affiliations
- Shanghai Medical College of Fudan University (CN)
- Corresponding author
- Shi, Guowei
- Venue
- Regenerative Therapy
- Publisher
- Elsevier BV
- Published
- 2023-03
- Volume
- 22
- Pages
- 136-147
- ISSN
- 2352-3204 (ISSN-L)
- PMC
- PMC9923042
- OpenAlex
- W4318428412
- Cited by
- 7
- References
- 49
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Regenerative Therapy (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Science and Technology Commission of Shanghai Municipality (20S31902600)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Surgery › Surgical Sutures and Adhesives
- Keywords
- fibrinogen, poly(L-lactide-co-ε-caprolactone), nanofibrous scaffolds, urethral injury, hypospadias, epithelial cell adhesion, urethral stricture, vascularization, tissue engineering scaffolds
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