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Cited by 389

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Yeast surface display for protein engineering and characterization

Open access
Gai, S Annie, Wittrup, K Dane·2007·Current Opinion in Structural Biology
DOI 10.1016/j.sbi.2007.08.012
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Abstract

Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein–protein interactions, represents a significant recent advance.

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Metadata

Authors
Gai, S. A., & Wittrup, K. D.
Venue
Current Opinion in Structural Biology
Publisher
Elsevier BV
Published
2007-08
Volume
17
Issue
4
Pages
467-473
ISSN
0959-440X (ISSN-L)
DOI
10.1016/j.sbi.2007.08.012
OpenAlex
W1971596340
Cited by
389
References
0
FWCI
14.36
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Yeast surface display for protein engineering and characterization. https://app.cochat.ai/papers/10.1016/j.sbi.2007.08.012
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Gai, S. A., & Wittrup, K. D. (2007). Yeast surface display for protein engineering and characterization. Current Opinion in Structural Biology. https://doi.org/10.1016/j.sbi.2007.08.012

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.