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Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity
Abstract
Biosorption of uranium(VI) by the green alga Chlorella vulgaris was studied at varying uranium concentrations from 5 μM to 1mM, and in the environmentally relevant pH range of 4.4 to 7.0. Living cells bind in a 0.1mM uranium solution at pH 4.4 within 5 min 14.3 ± 5.5 mg U/g dry biomass and dead cells 28.3 ± 0.6 mg U/g dry biomass which corresponds to 45% and 90% of total uranium in solution, respectively. During 96 h of incubation with uranium initially living cells died off and with 26.6 ± 2.1 mg U/g dry biomass bound similar amounts of uranium compared to dead cells, binding 27.0 ± 0.7 mg U/g dry biomass. In both cases, these amounts correspond to around 85% of the initially applied uranium. Interestingly, at a lower and more environmentally relevant uranium concentration of 5 μM, living cells firstly bind with 1.3 ± 0.2 mg U/g dry biomass to 1.4 ± 0.1 mg U/g dry biomass almost all uranium within the first 5 min of incubation. But then algal cells again mobilize up to 80% of the bound uranium during ongoing incubation in the time from 48 h to 96 h. The release of metabolism related substances is suggested to cause this mobilization of uranium. As potential leachates for algal-bound uranium oxalate, citrate and ATP were tested and found to be able to mobilize more than 50% of the algal-bound uranium within 24h. Differences in complexation of uranium by active and inactive algae cells were investigated with a combination of time-resolved laser-induced fluorescence spectroscopy (TRLFS), extended X-ray absorption fine structure (EXAFS) spectroscopy and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Obtained results demonstrated an involvement of carboxylic and organic/inorganic phosphate groups in the uranium complexation with varying contributions dependent on cell status, uranium concentration and pH.
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“The sensitivity of off-line MIR measurements can also elucidate molecular changes within an organism facilitated by metal sorption [58-60], which may be useful to understand the accumulation of metals of microalgae treating wastewaters.”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [58]
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Metadata
- Authors
- Vogel, M., Günther, A., Rossberg, A., Li, B., Bernhard, G., & Raff, J.
- Affiliations
- Helmholtz-Zentrum Dresden-Rossendorf (DE)
- Corresponding author
- Vogel, M.
- Venue
- Science of The Total Environment
- Publisher
- Elsevier BV
- Published
- 2010-12-15
- Volume
- 409
- Issue
- 2
- Pages
- 384-395
- ISSN
- 0048-9697 (ISSN-L)
- OpenAlex
- W2008380066
- Cited by
- 149
- References
- 72
- Citations by year
- 2026: 6 · 2025: 12 · 2024: 13 · 2023: 8 · 2022: 9 · 2021: 16 · 2020: 4 · 2019: 11 · 2018: 14 · 2017: 7 · 2016: 8 · 2015: 9 · 2014: 14 · 2013: 4 · 2012: 10
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Science of The Total Environment (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Inorganic Chemistry › Radioactive element chemistry and processing
- Physical Sciences › Environmental Science › Water Science and Technology › Adsorption and biosorption for pollutant removal
- Physical Sciences › Physics and Astronomy › Radiation › Radioactivity and Radon Measurements
- Keywords
- Chlorella vulgaris, green algae, pH dependence, uranium mobilization, citrate, ATP, phosphate complexation, carboxyl groups, uranium complexation, uranium leaching, time-resolved laser-induced fluorescence, EXAFS, ATR-FTIR spectroscopy
- MeSH terms
- Fresh Water, Hydrogen-Ion Concentration, Uranium, Water Pollutants, Radioactive, Chlorella vulgaris
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