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Cited by 46Constructed Wetlands for Wastewater TreatmentWastewater Treatment and Nitrogen RemovalWastewater Treatment and Reuse

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Unraveling the active microbial populations involved in nitrogen utilization in a vertical subsurface flow constructed wetland treating urban wastewater

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Pelissari, Catiane, Guivernau, Miriam, Viñas, Marc, de Souza, Samara Silva, García, Joan, Sezerino, Pablo Heleno, Ávila, Cristina·2017·Science of The Total Environment
DOI 10.1016/j.scitotenv.2017.01.091
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Abstract

The dynamics of the active microbial populations involved in nitrogen transformation in a vertical subsurface flow constructed wetland (VF) treating urban wastewater was assessed. The wetland (1.5m 2 ) operated under average loads of 130gCODm -2 d -1 and 17gTNm -2 d -1 in Period I, and 80gCODm -2 d -1 and 19gTNm -2 d -1 in Period II. The hydraulic loading rate (HLR) was 375mmd -1 and C/N ratio was 2 in both periods. Samples for microbial characterization were collected from the filter medium (top and bottom layers) of the wetland, water influent and effluent at the end of Periods I (Jun-Oct) and II (Nov-Jan). The combination of qPCR and high-throughput sequencing (NGS, MiSeq) assessment at DNA and RNA level of 16S rRNA genes and nitrogen-based functional genes (amoA and nosZ-clade I) revealed that nitrification was associated both with ammonia-oxidizing bacteria (AOB) (Nitrosospira) and ammonia-oxidizing archaea (AOA) (Nitrososphaeraceae), and nitrite-oxidizing bacteria (NOB) such as Nitrobacter. Considering the active abundance (based in amoA transcripts), the AOA population revealed to be more stable than AOB in both periods and depths of the wetland, being less affected by the organic loading rate (OLR). Although denitrifying bacteria (nosZ copies and transcripts) were actively detected in all depths, the denitrification process was low (removal of 2gTNm -2 d -1 for both periods) concomitant with NO x -N accumulation in the effluent. Overall, AOA, AOB and denitrifying bact…

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Research works printed but not linked: 54

  1. Adrados, 2014, Microbial communities from different types of natural wastewater treatment systems: vertical and horizontal flow constructed wetlands and biofilters, Water Res., 55, 304, 10.1016/j.watres.2014.02.011 · DOI
  2. Andrews, 1986, r and K-selection and microbial ecology, Adv. Microb. Ecol., 9, 99, 10.1007/978-1-4757-0611-6_3 · DOI
  3. Angnes, 2013, Bioresource technology correlating denitrifying catabolic genes with N2O and N2 emissions from swine slurry composting, Bioresour. Technol., 140, 368, 10.1016/j.biortech.2013.04.112 · DOI
  4. Ávila, 2017, Enhancement of total nitrogen removal through effluent recirculation and fate of PPCPs in a hybrid constructed wetland system treating urban wastewater, Sci. Total Environ., 584-585, 414, 10.1016/j.scitotenv.2017.01.024 · DOI
  5. Ávila, 2016, Influence of hydraulic loading rate, simulated storm events and seasonality on the treatment performance of an experimental three-stage hybrid constructed wetland system, Ecol. Eng., 87, 324, 10.1016/j.ecoleng.2015.11.042 · DOI
  6. Ávila, 2014, Attenuation of emerging organic contaminants in a hybrid constructed wetland system under different hydraulic loading rates and their associated toxicological effects in wastewater, Sci. Total Environ., 470, 1272, 10.1016/j.scitotenv.2013.10.065 · DOI
  7. Ávila, 2013, Three-stage hybrid constructed wetland system for wastewater treatment and reuse in warm climate regions, Ecol. Eng., 61, 43, 10.1016/j.ecoleng.2013.09.048 · DOI
  8. Calderer, 2014, Effects of enhanced denitrification on hydrodynamics and microbial community structure in a soil column system, Chemosphere, 111, 112, 10.1016/j.chemosphere.2014.03.033 · DOI
  9. Calheiros, 2007, Constructed wetland systems vegetated with different plants applied to the treatment of tannery wastewater, Water Res., 41, 1790, 10.1016/j.watres.2007.01.012 · DOI
  10. Caporaso, 2010, QIIME allows analysis of high-throughput community sequencing data, Nat. Methods, 7, 335, 10.1038/nmeth.f.303 · DOI · Paper page
  11. Caporaso, 2010, PyNAST: a flexible tool for aligning sequences to a template alignment, Bioinformatics, 26, 266, 10.1093/bioinformatics/btp636 · DOI
  12. Correa-Galeote, 2013, Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments, FEMS Microbiol. Ecol., 83, 340, 10.1111/j.1574-6941.2012.01479.x · DOI
  13. Cui, 2010, Removal of nutrients from wastewater with Canna indica L. under different vertical-flow constructed wetland conditions, Ecol. Eng., 36, 1083, 10.1016/j.ecoleng.2010.04.026 · DOI
  14. DeSantis, 2006, Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB, Appl. Environ. Microbiol., 72, 5069, 10.1128/AEM.03006-05 · DOI · Paper page
  15. Edgar, 2010, Search and clustering orders of magnitude faster than BLAST, Bioinformatics, 26, 2460, 10.1093/bioinformatics/btq461 · DOI
  16. Fan, 2016, Comparing abundance, composition and environmental influences on prokaryotic ammonia oxidizers in two subtropical constructed wetlands, Ecol. Eng., 90, 336, 10.1016/j.ecoleng.2016.01.034 · DOI
  17. Foladori, 2015, Bacteria viability and decay in water and soil of vertical subsurface flow constructed wetlands, Ecol. Eng., 82, 49, 10.1016/j.ecoleng.2015.04.058 · DOI
  18. García, 2010, Contaminant removal processes in subsurface-flow constructed wetlands: a review, Environ. Sci. Technol., 40, 561
  19. Graf, 2014, Intergenomic comparisons highlight modularity of the denitrification pathway and underpin the importance of community structure for N2O emissions, PLoS One, 9, 114, 10.1371/journal.pone.0114118 · DOI
  20. Guan, 2015, Influence of substrate type on microbial community structure in vertical-flow constructed wetland treating polluted river water, Environ. Sci. Pollut. Res., 22, 16202, 10.1007/s11356-015-5160-9 · DOI
  21. Haas, 2011, Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons, Genome Res., 21, 494, 10.1101/gr.112730.110 · DOI
  22. Headley, 2005, Treatment at different depths and vertical mixing within a 1-m deep horizontal subsurface flow wetland, Ecol. Eng., 25, 567, 10.1016/j.ecoleng.2005.07.012 · DOI
  23. Jin, 2010, Characterization and quantification of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a nitrogen-removing reactor using T-RFLP and qPCR, Appl. Microbiol. Biotechnol., 87, 1167, 10.1007/s00253-010-2595-2 · DOI
  24. Konneke, 2005, Isolation of an autotrophic ammonia-oxidizing marine archaeon, Nature, 437, 543, 10.1038/nature03911 · DOI
  25. Kozlowski, 2016, Comparison of nitrogen oxide metabolism among diverse ammonia-oxidizing bacteria, Front. Microbiol., 7, 1, 10.3389/fmicb.2016.01090 · DOI
  26. Lueders, 2006, Identification of bacterial micropredators distinctively active in a soil microbial food web, Appl. Environ. Microbiol., 72, 5342, 10.1128/AEM.00400-06 · DOI
  27. Meng, 2014, How to increase microbial degradation in constructed wetlands: influencing factors and improvement measures, Bioresour. Technol., 157, 316, 10.1016/j.biortech.2014.01.095 · DOI
  28. Molle, 2008, Potential for total nitrogen removal by combining vertical flow and horizontal flow constructed wetlands: a full-scale experiment study, Ecol. Eng., 34, 23, 10.1016/j.ecoleng.2008.05.016 · DOI
  29. Murphy, 2016, Nitrification cessation and recovery in an aerated saturated vertical subsurface flow treatment wetland: field studies and microscale biofilm modeling, Bioresour. Technol., 209, 125, 10.1016/j.biortech.2016.02.065 · DOI
  30. Paranychianakis, 2016, Pathways regulating the removal of nitrogen in planted and unplanted subsurface flow constructed wetlands, Water Res., 102, 321, 10.1016/j.watres.2016.06.048 · DOI
  31. Paredes, 2007, New aspects of microbial nitrogen transformations in the context of wastewater treatment - a review, Eng. Life Sci., 7, 13, 10.1002/elsc.200620170 · DOI
  32. Pelissari, 2017, Nitrogen transforming bacteria within a partially saturated vertical subsurface flow constructed wetland at full scale, Sci. Total Environ., 574, 390, 10.1016/j.scitotenv.2016.08.207 · DOI
  33. Pelissari, 2016, Organic load and hydraulic regime influence over the bacterial community responsible for the nitrogen cycling in bed media of vertical subsurface flow constructed wetland, Ecol. Eng., 95, 180, 10.1016/j.ecoleng.2016.06.079 · DOI
  34. Pester, 2012, AmoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions, Environ. Microbiol., 14, 525, 10.1111/j.1462-2920.2011.02666.x · DOI
  35. Platzer, 1999, Design recommendations for subsurface flow constructed wetlands for nitrification and denitrification, Water Sci. Technol., 40, 257, 10.2166/wst.1999.0170 · DOI
  36. Prenafeta-Boldú, 2012, Fungal/bacterial interactions during the biodegradation of TEX hydrocarbons (toluene, ethylbenzene and p-xylene) in gas biofilters operated under xerophilic conditions, FEMS Microbiol. Ecol., 80, 722, 10.1111/j.1574-6941.2012.01344.x · DOI
  37. Prosser, 2012, Archaeal and bacterial ammonia-oxidisers in soil: the quest for niche specialization and differentiation, Trends Microbiol., 20, 523, 10.1016/j.tim.2012.08.001 · DOI
  38. Reeder, 2010, Rapidly denoising pyrosequencing amplicon reads by exploiting rank-abundance distributions, Nat. Methods, 7, 668, 10.1038/nmeth0910-668b · DOI
  39. Rotthauwe, 1997, The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations, Appl. Environ. Microbiol., 63, 4704, 10.1128/aem.63.12.4704-4712.1997 · DOI
  40. Saeed, 2012, A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands: dependency on environmental parameters, operating conditions and supporting media, J. Environ. Manag., 112, 429, 10.1016/j.jenvman.2012.08.011 · DOI
  41. Saeed, 2011, Kinetic modelling of nitrogen and organics removal in vertical and horizontal flow wetlands, Water Res., 45, 3137, 10.1016/j.watres.2011.03.031 · DOI
  42. Salomo, 2009, Evaluation of the metabolic diversity of microbial communities in four different filter layers of a constructed wetland with vertical flow by Biolog analysis, Water Res., 43, 4569, 10.1016/j.watres.2009.08.009 · DOI
  43. Sauder, 2016, Nitric oxide scavengers differentially inhibit ammonia oxidation in ammonia-oxidizing archaea and bacteria, FEMS Microbiol. Lett., 363, 386, 10.1093/femsle/fnw052 · DOI
  44. Sezerino, 2012, Constructed wetlands and sand filter applied as onsite post-treatment of anaerobic effluent, Water Pract. Technol., 7, 1, 10.2166/wpt.2012.047 · DOI
  45. Sims, 2012, Temporal and spatial distributions of ammonia-oxidizing archaea and bacteria and their ratio as an indicator of oligotrophic conditions in natural wetlands, Water Res., 46, 4121, 10.1016/j.watres.2012.05.007 · DOI
  46. Tietz, 2007, Diversity of ammonia-oxidizing bacteria in a vertical flow constructed wetland, Water Sci. Technol., 56, 241, 10.2166/wst.2007.505 · DOI
  47. Tietz, 2008, Bacterial carbon utilization in vertical subsurface flow constructed wetlands, Water Res., 42, 1622, 10.1016/j.watres.2007.10.011 · DOI
  48. Vymazal, 2013, The use of hybrid constructed wetlands for wastewater treatment with special attention to nitrogen removal: a review of a recent development, Water Res., 47, 4795, 10.1016/j.watres.2013.05.029 · DOI
  49. Winter, 2003, The impact of sewage composition on the soil clogging phenomena of vertical flow constructed wetlands, Water Sci. Technol., 48, 9, 10.2166/wst.2003.0268 · DOI
  50. Wu, 2016, Bioresource technology intensified organics and nitrogen removal in the intermittent-aerated constructed wetland using a novel sludge-ceramsite as substrate, Bioresour. Technol., 210, 101, 10.1016/j.biortech.2016.01.051 · DOI
  51. Wuchter, 2006, Archaeal nitrification in the ocean, Proc. Natl. Acad. Sci. U. S. A., 103, 12317, 10.1073/pnas.0600756103 · DOI
  52. You, 2009, Ammonia-oxidizing archaea involved in nitrogen removal, Water Res., 43, 1801, 10.1016/j.watres.2009.01.016 · DOI
  53. Zhi, 2014, Quantitative response relationships between nitrogen transformation rates and nitrogen functional genes in a tidal flow constructed wetland under C/N ratio constraints, Water Res., 64, 32, 10.1016/j.watres.2014.06.035 · DOI
  54. Zhu, 2013, Ammonia oxidation pathways and nitrifier denitrification are significant sources of N2O and NO under low oxygen availability, Proc. Natl. Acad. Sci. U. S. A., 110, 6328, 10.1073/pnas.1219993110 · DOI

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Metadata

Authors
Pelissari, C., Guivernau, M., Viñas, M., de Souza, S. S., García, J., Sezerino, P. H., & Ávila, C.
Affiliations
  • Universidade Federal de Santa Catarina (BR)
  • Institute of Agrifood Research and Technology (ES)
  • Universitat de Girona (ES)
Corresponding author
Ávila, Cristina
ORCID
Venue
Science of The Total Environment
Publisher
Elsevier BV
Published
2017-04
Volume
584-585
Pages
642-650
ISSN
0048-9697 (ISSN-L)
DOI
10.1016/j.scitotenv.2017.01.091
OpenAlex
W2585286569
Cited by
46
References
0
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2023: 4 · 2022: 7 · 2021: 4 · 2020: 10 · 2019: 4 · 2018: 8 · 2017: 5
FWCI
7.70
Type
journal article
Language
en
Open access
Licence
Locations
  • The Science of The Total Environment (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (acceptedVersion, cc-by-nc-nd)
  • RECERCAT (Consorci de Serveis Universitaris de Catalunya) (acceptedVersion, other-oa)
  • UPCommons institutional repository (Universitat Politècnica de Catalunya) (submittedVersion, cc-by-nc-nd)
Funders
  • Ministry of Economy and Competitiveness (FJCI-2014-22245)
  • Coordination for the Improvement of Higher Level (CAPES-PDSE 99999.006475/2015-09)
Topics
  • Physical Sciences › Environmental Science › Waste Management and Disposal › Constructed Wetlands for Wastewater Treatment
  • Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
  • Physical Sciences › Environmental Science › Waste Management and Disposal › Wastewater Treatment and Reuse
Keywords
vertical flow constructed wetlands, municipal wastewater treatment, nitrogen removal, nitrification, denitrification, ammonia-oxidizing archaea, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, nosZ, Nitrosospira, Nitrobacter, organic loading rate, hydraulic loading rate, microbial community dynamics, biofilm
MeSH terms
Ammonia, Archaea, Bacteria, Genes, Bacterial, Nitrogen, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S, Water Microbiology, Water Purification, Genes, Archaeal, Wetlands, Wastewater

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CoChat. (2026). Citation check: Unraveling the active microbial populations involved in nitrogen utilization in a vertical subsurface flow constructed wetland treating urban wastewater. https://app.cochat.ai/papers/10.1016/j.scitotenv.2017.01.091
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Pelissari, C., Guivernau, M., Viñas, M., de Souza, S. S., García, J., Sezerino, P. H., & Ávila, C. (2017). Unraveling the active microbial populations involved in nitrogen utilization in a vertical subsurface flow constructed wetland treating urban wastewater. Science of The Total Environment. https://doi.org/10.1016/j.scitotenv.2017.01.091