The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Direct Reprogramming of Adult Human Fibroblasts to Functional Neurons under Defined Conditions
Open accessAbstract
SUMMARY Human induced pluripotent stem cells (hiPSCs) have been generated by reprogramming a number of different somatic cell types using a variety of approaches. In addition, direct reprogramming of mature cells from one lineage to another has emerged recently as an alternative strategy for generating cell types of interest. Here we show that a combination of a microRNA (miR-124) and two transcription factors (MYT1L and BRN2) is sufficient to directly reprogram postnatal and adult human primary dermal fibroblasts (mesoderm) to functional neurons (ectoderm) under precisely defined conditions. These human induced neurons (hiNs) exhibit typical neuronal morphology and marker gene expression, fire action potentials, and produce functional synapses between each other. Our findings have major implications for cell-replacement strategies in neurodegenerative diseases, disease modeling, and neural developmental studies.
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Cites
Direct conversion of fibroblasts to functional neurons by defined factors
Vierbuchen, T., Ostermeier, A., Pang, Z. P., Kokubu, Y., Südhof, T. C., & Wernig, M. (2010) · Nature · doi:10.1038/nature08797 · Cited by 3,017
Makeyev, E. V., Zhang, J., Carrasco, M. A., & Maniatis, T. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.07.015 · Cited by 1,395
miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche
Cheng, L.-C., Pastrana, E., Tavazoie, M., & Doetsch, F. (2009) · Nature Neuroscience · doi:10.1038/nn.2294 · Cited by 1,001
Reciprocal actions of REST and a microRNA promote neuronal identity
Conaco, C., Otto, S., Han, J.-J., & Mandel, G. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0511041103 · Cited by 736
The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development
Visvanathan, J., Lee, S., Lee, B., Lee, J. W., & Lee, S.-K. (2007) · Genes & Development · doi:10.1101/gad.1519107 · Cited by 683
MicroRNA-mediated switching of chromatin-remodelling complexes in neural development
Yoo, A. S., Staahl, B. T., Chen, L., & Crabtree, G. R. (2009) · Nature · doi:10.1038/nature08139 · Cited by 645
PU.1 and C/EBPα/β convert fibroblasts into macrophage-like cells
Feng, R., Desbordes, S. C., Xie, H., Tillo, E. S., Pixley, F., Stanley, E. R., & Graf, T. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0711961105 · Cited by 348
Cited by
Super-enhancers in the control of cell identity and disease
Hnisz, D., Abraham, B. J., Lee, T. I., Lau, A., Saint-André, V., Sigova, A. A., Hoke, H. A., & Young, R. A. (2013) · Cell · doi:10.1016/j.cell.2013.09.053 · Cited by 3,662
Rapid single-step induction of functional neurons from human pluripotent stem cells
Zhang, Y., Pak, C., Han, Y., Ahlenius, H., Zhang, Z., Chanda, S., Marro, S., Patzke, C., Acuna, C., Covy, J., et al. (2013) · Neuron · doi:10.1016/j.neuron.2013.05.029 · Cited by 1,644
Guo, Z., Zhang, L., Wu, Z., Chen, Y., Wang, F., & Chen, G. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2013.12.001 · Cited by 832
Hierarchical Mechanisms for Direct Reprogramming of Fibroblasts to Neurons
Wapinski, O. L., Vierbuchen, T., Qu, K., Lee, Q. Y., Chanda, S., Fuentes, D. R., Giresi, P. G., Ng, Y. H., Marro, S., Neff, N. F., et al. (2013) · Cell · doi:10.1016/j.cell.2013.09.028 · Cited by 627
Engineered In Vitro Disease Models
Benam, K. H., Dauth, S., Hassell, B., Herland, A., Jain, A., Jang, K.-J., Karalis, K., Kim, H. J., MacQueen, L., Mahmoodian, R., et al. (2015) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev-pathol-012414-040418 · Cited by 554
Metadata
- Authors
- Ambasudhan, R., Talantova, M., Coleman, R., Yuan, X., Zhu, S., Lipton, S. A., & Ding, S.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2011-08
- Volume
- 9
- Issue
- 2
- Pages
- 113-118
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W4246383450
- Cited by
- 481
- References
- 7
- FWCI
- 21.63
- Type
- journal article
- Language
- en
- Open access
- bronze
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