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The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development

Visvanathan, Jaya, Lee, Seunghee, Lee, Bora, Lee, Jae W., Lee, Soo-Kyung·2007·Genes & Development
DOI 10.1101/gad.1519107
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Abstract

Neuronal gene expression is tightly regulated in developing CNS. Here, we demonstrate the anti-neural function of phosphatase SCP1 (small C-terminal domain phosphatase 1) during development. We further show that the neuron-enriched microRNA miR-124 directly targets SCP1-3′ untranslated region (UTR) to suppress SCP1 expression. In developing spinal cord, expression of miR-124 and SCP1 is complementary, and miR-124 antagonism phenocopies SCP1 overexpression and vice versa. In P19 cells, miR-124 suppresses SCP1 expression and induces neurogenesis, and SCP1 counteracts this proneural activity of miR-124. Our results suggest that, during CNS development, timely down-regulation of SCP1 is critical for inducing neurogenesis, and miR-124 contributes to this process at least in part by down-regulating SCP1 expression.

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Crossref · 10.1101/gad.1519107

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  • miR-124a expression can be detected in E11.5 mouse embryos and continues to be expressed in neurons of adult mice.

    “While miR-124a expression can be detected in E11.5 mouse embryos and it continues to be expressed in neurons of adult mice (Miska et al. 2004;Visvanathan et al. 2007)”

    From MicroRNA expression in the adult mouse central nervous system (2008) · cites this paper as Visvanathan et al. 2007

  • In the developing chick neural tube, miR-124a is a component of a regulatory network that controls the transition between neural progenitors and post-mitotic neurons by suppressing the anti-neural factor SCP1.

    “In the developing chick neural tube, miR-124a is a component of a regulatory network, which controls the transition between neural progenitors and post-mitotic neurons, by suppressing the anti-neural factor SCP1 (Visvanathan et al. 2007).”

    From MicroRNA expression in the adult mouse central nervous system (2008) · cites this paper as Visvanathan et al. 2007

  • miR-124 promotes differentiation of neuronal progenitors to mature neurons by targeting small C-terminal domain phosphatase-1 and polypyrimidine tract-binding protein-1.

    “It promotes differentiation of neuronal progenitors to mature neurons by targeting small C-terminal domain phosphatase-1 and polypyrimidine tract–binding protein-1 that repress expression of neuronal genes in non-neuronal cells18,20,40.”

    From MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-α–PU.1 pathway (2011) · cites this paper as [40]

  • miR-124 is expressed in the CNS by neuronal cells.

    “miR-124 is known to be expressed in the CNS by neuronal cells23,39–41.”

    From MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-α–PU.1 pathway (2011) · cites this paper as [40]

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Research works printed but not linked: 3

  1. Andres, M.E., Burger, C., Peral-Rubio, M.J., Battaglioli, E., Anderson, M.E., Grimes, J., Dallman, J., Ballas, N., and Mandel, G. 1999. CoREST: A functional corepressor required for regulation of neural- specific gene expression. Proc. Natl. Acad. Sci. 96: 9873-9878.
  2. Conaco, C., Otto, S., Han, J.J., and Mandel, G. 2006. Reciprocal actions of REST and a microRNA promote neuronal identity. Proc. Natl. Acad. Sci. 103: 2422-2427.
  3. Paquette, A.J., Perez, S.E., and Anderson, D.J. 2000. Constitutive expres- sion of the neuron-restrictive silencer factor (NRSF)/REST in differ- entiating neurons disrupts neuronal gene expression and causes axon pathfinding errors in vivo. Proc. Natl. Acad. Sci. 97: 12318-12323.

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Authors
Visvanathan, J., Lee, S., Lee, B., Lee, J. W., & Lee, S.-K.
Venue
Genes & Development
Publisher
Cold Spring Harbor Laboratory
Published
2007-04-01
Volume
21
Issue
7
Pages
744-749
ISSN
  • 0890-9369
  • 1549-5477
DOI
10.1101/gad.1519107
Cited by
579
References
2
Language
en

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