Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics
Open accessAbstract
Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, 3D environmental cues, and modes of multicellular association. In this Protocol Review, we dissect these growth components by comparing cell culture methods and identifying the benefits and pitfalls associated with each one. We further provide criteria, considerations, and suggestions to achieve optimal cell growth for hPSC expansion, differentiation, and use in future therapeutic applications.
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Cell line variability is a commonly cited shortcoming of organoid systems that rely on tissue self-assembly.
“Cell line variability is a commonly cited shortcoming of organoid systems that rely on tissue self-assembly (Chen et al., 2014; Völkner et al., 2016).”
From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Chen et al., 2014
The ‘Emerging Coculture Systems for Tissue Morphogenesis’ section describes cerebral organoids forming through self-organization in 3D culture. The ‘Benefits and pitfalls of suspension culture’ section discusses variability and sensitivity of some stem-cell lines, but does not identify cell-line variability as a shortcoming of self-assembling organoids. Page numbers are unavailable in our copy. (LLM, conf=0.82)
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- Authors
- Chen, K. G., Mallon, B. S., McKay, R. D. G., & Robey, P. G.
- Affiliations
- National Institutes of Health (US)
- Lieber Institute for Brain Development (US)
- Corresponding author
- Chen, Kevin G.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2014-01
- Volume
- 14
- Issue
- 1
- Pages
- 13-26
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- PMC
- PMC3915741
- OpenAlex
- W2045240673
- Cited by
- 358
- References
- 110
- Citations by year
- 2026: 16 · 2025: 13 · 2024: 20 · 2023: 22 · 2022: 36 · 2021: 38 · 2020: 33 · 2019: 47 · 2018: 30 · 2017: 33 · 2016: 29 · 2015: 26 · 2014: 15
- FWCI
- 3.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell stem cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (Z99 NS999999)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- human pluripotent stem cells, stem cell culture, cell expansion, growth factors, extracellular matrix, 3D cell culture, cell differentiation, regenerative medicine, stem cell therapy, large-scale culture
- MeSH terms
- Cell Differentiation, Humans, Cell Culture Techniques, Pluripotent Stem Cells
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