Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components
Abstract
We have recently demonstrated the formation of interconnecting canalicular cell processes in bone cells upon contact with basement membrane components. Here we have determined whether growth factors in the reconstituted basement membrane (Matrigel) were active in influencing the cellular network formation. Various growth factors including transforming growth factor beta (TGF-beta), epidermal growth factor (EGF), insulin-like growth factor 1, bovine fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) were identified in Matrigel. Exogenous TGF-beta blocked the cellular network formation. Conversely, addition of TGF-beta 1 neutralizing antibodies to Matrigel stimulated the cellular network formation. bFGF, EGF, and PDGF all promoted cellular migration and organization on Matrigel. Addition of bFGF to MC3T3-E1 cells grown on Matrigel overcame the inhibitory effect of TGF-beta. Some TGF-beta remained bound to type IV collagen purified from the Engelbreth-Holm-Swarm tumor matrix. These data demonstrate that reconstituted basement membrane contains growth factors which influence cellular behavior, suggesting caution in the interpretation of experiments on cellular activity related to Matrigel, collagen type IV, and possibly other extracellular matrix components.
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Matrigel contains growth factors such as TGF-β, EGF, IGF-1, bFGF, and PDGF.
“A critical issue in the use of Matrigel is that it contains growth factors, like e.g. transforming growth factor β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor 1, bovine fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) (Vukicevic et al., 1992[43]).”
From Optimization of extracellular matrix for primary human hepatocyte cultures using mixed collagen-matrigel matrices (2023) · cites this paper as Vukicevic et al., 1992[43]
Lot-to-lot variations of Matrigel compound the difficulty of identifying the signals necessary for organoid structure and function.
“The undefined nature of Matrigel makes it difficult to identify the signals necessary for organoid structure and function; this difficulty is compounded by lot-to-lot variations of Matrigel45–48.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [46]
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“A critical issue in the use of Matrigel is that it contains growth factors, like e.g. transforming growth factor β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor 1, bovine fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) (Vukicevic et al., 1992).”
- Authors
- Vukicevic, S., Kleinman, H. K., Luyten, F. P., Roberts, A. B., Roche, N. S., & Reddi, A. H.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Kleinman, Hynda K.
- Venue
- Experimental Cell Research
- Publisher
- Elsevier BV
- Published
- 1992-09
- Volume
- 202
- Issue
- 1
- Pages
- 1-8
- ISSN
- 0014-4827 (ISSN-L)
- OpenAlex
- W2048321002
- Cited by
- 696
- References
- 37
- Citations by year
- 2026: 19 · 2025: 34 · 2024: 19 · 2023: 24 · 2022: 35 · 2021: 44 · 2020: 37 · 2019: 18 · 2018: 13 · 2017: 37 · 2016: 27 · 2015: 18 · 2014: 26 · 2013: 20 · 2012: 12
- FWCI
- 9.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- Matrigel, basement membrane, growth factors, TGF-beta, fibroblast growth factor, epidermal growth factor, platelet-derived growth factor, insulin-like growth factor I, type IV collagen, cell migration, MC3T3-E1 cells, bone cells, extracellular matrix
- MeSH terms
- Animals, Antibodies, Basement Membrane, Cell Line, Cell Movement, Collagen, Drug Combinations, Growth Substances, Immunohistochemistry, Kinetics, Laminin, Osteoblasts, Proteoglycans, Time Factors, Transforming Growth Factor beta
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