Derivation and FACS-Mediated Purification of PAX3+/PAX7+ Skeletal Muscle Precursors from Human Pluripotent Stem Cells
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Human pluripotent stem cells (hPSCs) constitute a promising resource for use in cell-based therapies and a valuable in vitro model for studying early human development and disease. Despite significant advancements in the derivation of specific fates from hPSCs, the generation of skeletal muscle remains challenging and is mostly dependent on transgene expression. Here, we describe a method based on the use of a small-molecule GSK3β inhibitor to derive skeletal muscle from several hPSC lines. We show that early GSK3β inhibition is sufficient to create the conditions necessary for highly effective derivation of muscle cells. Moreover, we developed a strategy for stringent fluorescence-activated cell sorting-based purification of emerging PAX3+/PAX7+ muscle precursors that are able to differentiate in postsort cultures into mature myocytes. This transgene-free, efficient protocol provides an essential tool for producing myogenic cells for in vivo preclinical studies, in vitro screenings, and disease modeling.
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Human pluripotent stem cells induced to express PAX7 and treated with defined chemical cocktails yield transplantable muscle progenitors that refill the satellite cell niche and maintain dystrophin-positive fibers in dystrophin-deficient mice.
“Induced expression of PAX7 and exposure to defined chemical cocktails145–147 in human pluripotent stem cells has been shown to generate engraftable muscle progenitor cells that replenish the satellite cell compartment and sustain formation of dystrophin-positive myofibers in dystrophin-deficient mice145,147.”
From Stem cell aging: mechanisms, regulators and therapeutic opportunities (2014) · cites this paper as [146]
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- Authors
- Borchin, B., Chen, J., & Barberi, T.
- Affiliations
- Australian Regenerative Medicine Institute (AU)
- Corresponding author
- Barberi, Tiziano
- ORCID
- Chen, Joseph
- Venue
- Stem Cell Reports
- Publisher
- Elsevier BV
- Published
- 2013-12
- Volume
- 1
- Issue
- 6
- Pages
- 620-631
- ISSN
- 2213-6711 (ISSN-L)
- PMC
- PMC3871395
- OpenAlex
- W2012799716
- Cited by
- 184
- References
- 40
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 8 · 2023: 18 · 2022: 17 · 2021: 22 · 2020: 15 · 2019: 11 · 2018: 15 · 2017: 18 · 2016: 14 · 2015: 19 · 2014: 9 · 2013: 1
- FWCI
- 6.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Reports (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Australian Government
- Australian Rotary Health
- State Government of Victoria
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- human pluripotent stem cells, skeletal muscle differentiation, Pax3, Pax7, myogenic precursors, GSK-3β inhibition, small molecule inhibitors, fluorescence-activated cell sorting, disease modeling, cell therapy
- MeSH terms
- Glycogen Synthase Kinase 3 beta, PAX3 Transcription Factor, Cell Differentiation, Flow Cytometry, Humans, RNA, Messenger, Muscle, Skeletal, Cell Culture Techniques, Gene Expression Profiling, Glycogen Synthase Kinase 3, Pluripotent Stem Cells, Paired Box Transcription Factors, PAX7 Transcription Factor
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