The effect of exogenous 24-epibrassinolide on the ecdysteroid content in the leaves of Spinacia oleracea L.
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The aim of this study was to show whether/how the application of exogenous 24-epibrassinolide can affect the content of ecdysteroids in spinach leaves. Brassinosteroids and ecdysteroids, structurally related phytosterols, show effect on a range of processes in plants. Brassinosteroids increase biomass yield in some species, photosynthesis and resistance to stress, and ecdysteroids show effect on proteins responsible for binding of CO2 or water cleavage. The mutual interaction of these sterols in plants is unclear. The UPLC-(+)ESI-MS/MS analyses of extracts of treated and untreated spinach (Spinacia oleracea L.) leaves show that the application of exogenous 24-epibrassinolide does influence the ecdysteroid content in plant tissues. The response differs for the major ecdysteroids and also differs from that for the minor ones and is dependent on the developmental stage of the leaves within the same plant or the 24-epibrassinolide concentration applied.
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Application of BRs reduces polypodine B and stachysterone content and enhances ajugasterone C content.
“However, the application of BRs reduces polypodine B and stachysterone content and enhances ajugasterone C content.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [217]
BR application leads to modulation of PE profiles inside plant tissues within hours.
“Thus, it can be concluded that BR application leads to modulation of PE profiles inside plant tissues within hours [217].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [217]
Exogenous application of BL changes the PE profile in younger leaves.
“Further, the exogenous application of BL changes the PE profile in younger leaves.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [217]
In younger leaves of the control set, a higher concentration of polypodine B is found, whereas in older leaves, lower contents of ajugasterone C and stachysterone are found.
“In younger leaves of the control set, a higher concentration of polypodine B is found, whereas in older leaves, lower contents of ajugasterone C and stachysterone are found.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [217]
PE concentration is higher in older leaves of the control set than in plants treated with 10−6 M BL, whereas 10−8 M BL induced PE content.
“It has been demonstrated that PE concentration is higher in older leaves of the control set than in plants treated with 10−6 M BL, whereas 10−8 M BL induced PE content.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [217]
Application of brassinolide (BL) (10−8 or 10−6 M) alters PE content to a certain degree, which largely depends on leaves' developmental stages.
“Application of brassinolide (BL) (10−8 or 10−6 M) alters PE content to a certain degree, which largely depends on leaves’ developmental stages.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [217]
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References 44 · Cited by 9
- Authors
- Kamlar, M., Rothova, O., Salajkova, S., Tarkowska, D., Drasar, P., Kocova, M., Harmatha, J., Hola, D., Kohout, L., & Macek, T.
- Affiliations
- University of Chemistry and Technology, Prague (CZ)
- University Hospital Hradec Králové (CZ)
- Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry (CZ)
- Charles University (CZ)
- Venue
- Steroids
- Publisher
- Elsevier BV
- Published
- 2015-05
- Volume
- 97
- Pages
- 107-112
- ISSN
- 0039-128X (ISSN-L)
- OpenAlex
- W2329204742
- Cited by
- 9
- References
- 44
- Citations by year
- 2026: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2016: 2
- FWCI
- 0.29
- Type
- journal article
- Language
- en
- Open access
- green
- asep.lib.cas.cz
- Licence
- public-domain
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Steroids (publishedVersion)
- PubMed (publishedVersion)
- ASEP (submittedVersion, public-domain)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Growth Enhancement Techniques
- Keywords
- 24-epibrassinolide, ecdysteroids, spinach, brassinosteroids, leaf development, phytosterols, leaf developmental stages, exogenous hormones
- MeSH terms
- Molecular Conformation, Steroids, Heterocyclic, Plant Leaves, Spinacia oleracea, Ecdysteroids, Brassinosteroids
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