Fast decellularization process using supercritical carbon dioxide for trabecular bone
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Duarte et al. demonstrated that the use of scCO2 with tri-n-butyl phosphate (TnBP) effectively reduced DNA content in decellularized porcine trabecular bone tissue.
“Duarte et al. demonstrated that the use of scCO2 with tri‐n‐butyl phosphate (TnBP) effectively reduced DNA content in decellularized porcine trabecular bone tissue, exemplifying the synergy between physical and chemical decellularization methods.”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [86]
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- Authors
- Duarte, M. M., Ribeiro, N., Silva, I. V., Dias, J. R., Alves, N. M., & Oliveira, A. L.
- Affiliations
- Universidade Católica Portuguesa (PT)
- Universidade de Leiria e Oeste (PT)
- Corresponding author
- Oliveira, Ana L.
- Venue
- The Journal of Supercritical Fluids
- Publisher
- Elsevier BV
- Published
- 2021-06
- Volume
- 172
- Pages
- 105194
- ISSN
- 0896-8446 (ISSN-L)
- OpenAlex
- W3129573659
- Cited by
- 22
- References
- 65
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 2 · 2022: 5 · 2021: 4
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- The Journal of Supercritical Fluids (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Funders
- European Commission (0245_IBEROS_1_E, PT2020)
- Pan African Materials Institute
- Fundação para a Ciência e a Tecnologia (01-0145, POCI-01-0145, PT2020, UIDB/04044/2020, POCI-01-0145-FEDER-, UID/Multi/50016/2019, 0245_IBEROS_1_E, POCI-01-, POCI-01-0145-FEDER-31146, CENTRO2020, CENTRO-01–0145-FEDER-000014, 022158)
- Interreg (0245_IBEROS_1_E)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- decellularization, supercritical carbon dioxide, trabecular bone, tributyl phosphate, porcine bone, bone scaffolds, DNA removal, cosolvents, tissue engineering
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