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Tales of Detailed Poly(A) Tails

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Nicholson, Angela L., Pasquinelli, Amy E.·2019·Trends in Cell Biology
DOI 10.1016/j.tcb.2018.11.002PMC PMC7083203
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Abstract

Poly(A) tails are non-templated additions of adenosines at the 3' ends of most eukaryotic mRNAs. In the nucleus, these RNAs are co-transcriptionally cleaved at a poly(A) site and then polyadenylated before being exported to the cytoplasm. In the cytoplasm, poly(A) tails play pivotal roles in the translation and stability of the mRNA. One challenge in studying poly(A) tails is that they are difficult to sequence and accurately measure. However, recent advances in sequencing technology, computational algorithms, and other assays have enabled a more detailed look at poly(A) tail length genome-wide throughout many developmental stages and organisms. With the help of these advances, our understanding of poly(A) tail length has evolved over the past 5 years with the recognition that highly expressed genes can have short poly(A) tails and the elucidation of the seemingly contradictory roles for poly(A)-binding protein (PABP) in facilitating both protection and deadenylation.

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Authors
Nicholson, A. L., & Pasquinelli, A. E.
Affiliations
University of California San Diego (US)
Corresponding author
Pasquinelli, Amy E.
ORCID
Venue
Trends in Cell Biology
Publisher
Elsevier BV
Published
2019-03
Volume
29
Issue
3
Pages
191-200
ISSN
0962-8924 (ISSN-L)
DOI
10.1016/j.tcb.2018.11.002
PMC
PMC7083203
OpenAlex
W2903167102
Cited by
209
References
3
Citations by year
2026: 21 · 2025: 22 · 2024: 31 · 2023: 31 · 2022: 37 · 2021: 33 · 2020: 27 · 2019: 7
FWCI
1.66
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Trends in Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Institute of General Medicine (T32 GM007240)
  • National Science Foundation Graduate Research Fellowship (DGE-1650112)
  • National Institutes of Health (R35 GM127012)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
poly(A) tail, mRNA 3' end, poly(A) site, polyadenylation, mRNA stability, translation (biology), poly(A)-binding protein, deadenylation, poly(A) tail length, gene expression, mRNA export
MeSH terms
Algorithms, Animals, Cell Nucleus, Cytoplasm, Humans, Poly A, RNA, Messenger, Sequence Analysis, RNA, Computational Biology

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