GnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterus
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In reproductive tissues, GnRH participates in the regulation of cell growth and proliferation by direct binding to the GnRH-R, which is essential for embryo implantation. However, there is no study on the expression and cellular localization of GnRH and GnRH-R in the canine uterus and placenta. Therefore, bitches were ovariohysterectomized 10 to 12 days after mating (vaginal cytology and progesterone measurement), the uteri were flushed, and if embryos were detectable, bitches were allocated to the embryo positive group (E-pos.; preimplantation, n = 5). Other bitches were operated at later stages and, dependent on the gestational age, either allotted to the post-implantation group (Day 18-25 after mating, n = 9), or the mid-gestation group (Day 30-40 after mating, n = 3). Dogs negative in embryo flushing served as controls (E-neg.; controls, n = 5). Samples of the entire uterine wall were taken from the middle of the horn in E-neg. and E-pos. groups, and from placental and interplacental uterine sites in post-implantation and mid-gestation groups. GnRH-R expression was localized at the mRNA and protein levels by immunohistochemistry and in situ hybridization. The expression of GnRH and GnRH-R mRNA was assessed by semiquantitative polymerase chain reaction. Additionally, both GnRH and GnRH-R mRNA were expressed in all tissues examined until mid-gestation. Relative expression of GnRH was higher than that of GnRH-R (P < 0.05). During the post-implantation stage, GnRH-R expression was significantly higher in uteroplacental than in interplacental tissues. In the uterus, GnRH-R stained strongly in the surface and glandular epithelial cells, and seemed to be weaker in myometrium and stroma. Placental signals were predominantly localized in fetal trophoblast cells and to a lesser extent in maternal decidual cells. These findings suggest a local regulatory function of GnRH during early canine pregnancy.
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GnRH and GnRHR have been detected in the placenta and decidua of several species.
“as GnRH and GnRHR have been detected in the placenta and decidua of several species23–28.”
From Conditional loss of ERK1 and ERK2 results in abnormal placentation and delayed parturition in the mouse (2019) · cites this paper as [25]
GnRHR expression in canine placenta is consistent with expression of Cre recombinase in the GRIC model by the endogenous GnRHR promoter in the mouse.
“These observations are entirely consistent with expression of Cre recombinase in the GRIC model by the endogenous GnRHR promoter in the mouse.”
From Conditional loss of ERK1 and ERK2 results in abnormal placentation and delayed parturition in the mouse (2019) · cites this paper as [25]
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Brown, J. L., Sones, J. L., Angulo, C. N., Abbott, K., Miller, A. D., Boehm, U., & Roberson, M. S. (2019) · Scientific Reports · doi:10.1038/s41598-019-45997-0 · Cited by 13 · see citing works
- Authors
- Schäfer-Somi, S., Kowalewski, M. P., Kanca, H., Bozkurt, M. F., Gram, A., Sabitzer, S., Kucukaslan, I., Ay, S. S., & Aslan, S.
- Affiliations
- University of Zurich (CH)
- Ankara University (TR)
- Afyon Kocatepe University (TR)
- University of Veterinary Medicine Vienna (AT)
- Ondokuz Mayıs University (TR)
- Near East University (CY)
- Venue
- Theriogenology
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 84
- Issue
- 9
- Pages
- 1482-1489
- ISSN
- 0093-691X (ISSN-L)
- OpenAlex
- W2106514213
- Cited by
- 12
- References
- 45
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 1
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Theriogenology (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
- Zurich Open Repository and Archive (University of Zurich)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Keywords
- gonadotropin-releasing hormone, GnRH receptor, canine placenta, canine uterus, embryo implantation, trophoblast, decidual cells, uterine epithelium, gene expression
- MeSH terms
- Animals, Dogs, Female, Gene Expression Regulation, Gonadotropin-Releasing Hormone, Placenta, Pregnancy, Receptors, LHRH, Uterus, Real-Time Polymerase Chain Reaction
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