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New substrates for TonB-dependent transport: do we only see the ‘tip of the iceberg’?
Abstract
TonB-dependent transport is a mechanism for active uptake across the outer membrane of Gram-negative bacteria. The system promotes transport of rare nutrients and was thought to be restricted to iron complexes and vitamin B12. Recent experimental evidence of TonB-energized transport of nickel and different carbohydrates, in addition to bioinformatic-based predictions, challenges this notion and reveals that the number and variety of TonB-dependent substrates is underestimated. It is becoming clear that the chemical nature of the substrates, the energetic requirements for transport and the subsequent translocation across the cytoplasmic membrane can differ from those of the well-studied systems for iron complexes and vitamin B12. These findings question the understanding of TonB-dependent uptake and provide insights into the adaptation of bacteria to their environments.
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“OM ferric siderophore receptors are TonB-dependent members of the porin superfamily.Hence, they are called TonB-dependent transporters (14) or ligand-gated porins [LGP (15)], in the latter case because ligand binding activates their TonB-depend ent transport process.”
From Siderophore-mediated iron acquisition by Klebsiella pneumoniae (2024) · cites this paper as [14]
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Metadata
- Authors
- Schauer, K., Rodionov, D. A., & de Reuse, H.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Russian Academy of Sciences (RU)
- Venue
- Trends in Biochemical Sciences
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 33
- Issue
- 7
- Pages
- 330-338
- ISSN
- 0968-0004 (ISSN-L)
- OpenAlex
- W2130630244
- Cited by
- 360
- References
- 70
- Citations by year
- 2026: 12 · 2025: 20 · 2024: 11 · 2023: 5 · 2022: 25 · 2021: 19 · 2020: 28 · 2019: 20 · 2018: 17 · 2017: 29 · 2016: 28 · 2015: 11 · 2014: 23 · 2013: 30 · 2012: 23
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Trends in Biochemical Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutscher Akademischer Austauschdienst
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- TonB-dependent transport, Gram-negative bacteria, iron complexes, vitamin B12, nickel uptake, carbohydrate transport, substrate specificity, bacterial adaptation
- MeSH terms
- Bacterial Proteins, Biological Transport, Membrane Proteins, Models, Biological, Phylogeny, Vitamin B 12
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