The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time
Open accessAbstract
Quantitative PCR (qPCR) is one of the most common techniques for quantification of nucleic acid molecules in biological and environmental samples. Although the methodology is perceived to be relatively simple, there are a number of steps and reagents that require optimization and validation to ensure reproducible data that accurately reflect the biological question(s) being posed. This review article describes and illustrates the critical pitfalls and sources of error in qPCR experiments, along with a rigorous, stepwise process to minimize variability, time, and cost in generating reproducible, publication quality data every time. Finally, an approach to make an informed choice between qPCR and digital PCR technologies is described.
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Citations
Cites
1 retracted
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Hernández-García, J., Carrillo-Carrasco, V. P., Rienstra, J., Tanaka, K., de Roij, M., Dipp-Álvarez, M., Freire-Ríos, A., Crespo, I., Boer, R., van den Berg, W. A. M., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-55278-8 · Cited by 46
Optimizations for identifying reference genes in bone and cartilage bioengineering
Xiong, F., Cheng, X., Zhang, C., Klar, R. M., & He, T. (2021) · BMC Biotechnology · doi:10.1186/s12896-021-00685-8 · Cited by 12
Nerusch, J., Schicht, G., Herzog, N., Küpper, J.-H., Seehofer, D., & Damm, G. (2025) · Cells · doi:10.3390/cells14161254 · Cited by 3
Metadata
- Authors
- Taylor, S. C., Nadeau, K., Abbasi, M., Lachance, C., Nguyen, M., & Fenrich, J.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2019-07
- Volume
- 37
- Issue
- 7
- Pages
- 761-774
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2910544292
- Cited by
- 830
- References
- 54
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Taylor, S. C., Nadeau, K., Abbasi, M., Lachance, C., Nguyen, M., & Fenrich, J. (2019). The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time. Trends in Biotechnology. https://doi.org/10.1016/j.tibtech.2018.12.002
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