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The Third Revolution in Sequencing Technology

van Dijk, Erwin L., Jaszczyszyn, Yan, Naquin, Delphine, Thermes, Claude·2018·Trends in Genetics
DOI 10.1016/j.tig.2018.05.008
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Abstract

Forty years ago the advent of Sanger sequencing was revolutionary as it allowed complete genome sequences to be deciphered for the first time. A second revolution came when next-generation sequencing (NGS) technologies appeared, which made genome sequencing much cheaper and faster. However, NGS methods have several drawbacks and pitfalls, most notably their short reads. Recently, third-generation/long-read methods appeared, which can produce genome assemblies of unprecedented quality. Moreover, these technologies can directly detect epigenetic modifications on native DNA and allow whole-transcript sequencing without the need for assembly. This marks the third revolution in sequencing technology. Here we review and compare the various long-read methods. We discuss their applications and their respective strengths and weaknesses and provide future perspectives.

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Metadata

Authors
van Dijk, E. L., Jaszczyszyn, Y., Naquin, D., & Thermes, C.
Venue
Trends in Genetics
Publisher
Elsevier BV
Published
2018-09
Volume
34
Issue
9
Pages
666-681
ISSN
0168-9525
DOI
10.1016/j.tig.2018.05.008
Cited by
1,057
References
6
Language
en
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