Biased codon usage in signal peptides: A role in protein export
Abstract
The signal peptide of proteins exported via the general secretory pathway encodes structural features that enable the targeting and export of the protein to the periplasm. Recent studies have shown biased codon usage at the second amino acid position and a high usage of non-optimal codons within the signal peptide. Altering these biases in codon usage can have deleterious effects on protein folding and export. We propose that these codon-usage biases act in concert to optimize the export process through modulating ribosome spacing on the transcript. This highlights a new aspect of protein export and implies that codon usage in the signal peptide encodes signals that are important for protein targeting and export to the periplasm.
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The presence of rare codons in Sec signal peptides is not casual but is highly important to ensure efficient interaction of export proteins with the secretory machinery and to prevent their degradation.
“These results confirmed that the presence of rare codons present in Sec signal peptides is not casual, but is highly important to ensure an efficient interaction of the export proteins with the components of the secretory machinery and also to prevent their degradation [150,151].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [150]
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Self-citation
- Authors
- Zalucki, Y. M., Beacham, I. R., & Jennings, M. P.
- Affiliations
- Emory University (US)
- Griffith University (AU)
- The University of Queensland (AU)
- Corresponding author
- Jennings, Michael P.
- Venue
- Trends in Microbiology
- Publisher
- Elsevier BV
- Published
- 2009-04
- Volume
- 17
- Issue
- 4
- Pages
- 146-150
- ISSN
- 0966-842X (ISSN-L)
- OpenAlex
- W2060100457
- Cited by
- 57
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 5 · 2019: 5 · 2018: 7 · 2017: 6 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 1
- FWCI
- 0.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Trends in Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Griffith Research Online (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- codon usage bias, signal peptide, protein export, general secretory pathway, periplasmic targeting, protein folding
- MeSH terms
- Amino Acid Sequence, Codon, Escherichia coli, Molecular Sequence Data, Protein Biosynthesis, Protein Folding, Protein Transport, Protein Sorting Signals, Escherichia coli Proteins
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