Contamination in Low Microbial Biomass Microbiome Studies: Issues and Recommendations
Eisenhofer, Raphael, Minich, Jeremiah J., Marotz, Clarisse, Cooper, Alan, Knight, Rob, Weyrich, Laura S.·2019·Trends in Microbiology
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- Additional file 4 of Signatures of landscape and captivity in the gut microbiota of Southern Hairy-nosed Wombats (Lasiorhinus latifrons)Raphael Eisenhofer, Helgen, Kristofer M., Taggart, David · 2021 · Figshare
- Additional file 2 of Using strain-resolved analysis to identify contamination in metagenomics dataYue Clare Lou, Jordan Hoff, Matthew R. Olm, West-Roberts, Jacob, Spencer Diamond, Brian Firek, Michael J. Morowitz, Jillian F. Banfield · 2023 · Figshare
- Additional file 12 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 2 of Repeatability and reproducibility assessment in a large-scale population-based microbiota study: case study on human milk microbiotaShirin Moossavi, Kelsey B. Fehr, Khafipour, Ehsan, Meghan B. Azad · 2021 · Figshare
- Additional file 2 of The lower airways microbiota and antimicrobial peptides indicate dysbiosis in sarcoidosisKristel Svalland Knudsen, Sverre Lehmann, Nielsen, Rune, Solveig Tangedal, Paytuvi-Gallart, Andreu, Walter Sanseverino, Einar Marius Hjellestad Martinsen, Hiemstra, Pieter S., Tomas Eagan · 2022 · Figshare
- Additional file 8 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 2 of Metagenomic analysis of healthy and diseased peri-implant microbiome under different periodontal conditions: a cross-sectional studySong, Liang, Feng, Ziying, Qianrong Zhou, Wu, Xingwen, Zhang, Limin, Yang Sun, Li, Ruixue, Chen, Huijuan, Fei Yang, Yu, Youcheng · 2024 · Figshare
- Additional file 4 of Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNAYacine Amar, Ilias Lagkouvardos, Silva, Rafaela L., Oluwaseun Ayodeji Ishola, Foesel, Bärbel U., Susanne Kublik, Anne Schöler, Sebastian Niedermeier, Rachela Bleuel, Alexander Zink et al. · 2021 · Figshare
- Additional file 2 of Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and decontamination methodsWang, Chun, Zhang, Li, Jiang, Xuan, Ma, Wentai, Geng, Hui, Xue Wang, Li, Mingkun · 2022 · Figshare
- Additional file 2 of Differences in airway microbiome and metabolome of single lung transplant recipientsSharma, Nirmal S., Vestal, Grant, Wille, Keith, Kapil Patel, Cheng, Feng, Srinivas M. Tipparaju, Sultan Tousif, Mudassir Meraj Banday, Xu, Xin, Wilson, Landon et al. · 2020 · Figshare
- Additional file 6 of Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNAYacine Amar, Ilias Lagkouvardos, Silva, Rafaela L., Oluwaseun Ayodeji Ishola, Foesel, Bärbel U., Susanne Kublik, Anne Schöler, Sebastian Niedermeier, Rachela Bleuel, Alexander Zink et al. · 2021 · Figshare
- Additional file 6 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 2 of N/S element transformation modulating lithospheric microbial communities by single-species manipulationShun Yao, Tianzhi Jin, Zhang, Lu, Zhang, Yong, Chen, Rui, Qian Wang, Mingjie Lv, Chuxiao Hu, Ma, Ting, Xia, Wenjie · 2024 · Figshare
- Additional file 1 of Using strain-resolved analysis to identify contamination in metagenomics dataYue Clare Lou, Jordan Hoff, Matthew R. Olm, Jacob A. West-Roberts, Spencer Diamond, Brian Firek, Michael J. Morowitz, Jillian F. Banfield · 2023 · Figshare
- Additional file 1 of Microbiota of the prostate tumor environment investigated by whole-transcriptome profilingPaul Vinu Salachan, Martin Rasmussen, Jacob Fredsøe, Ulhøi, Benedicte, Michael Borre, Karina Dalsgaard Sørensen · 2022 · Figshare
- Additional file 1 of Metagenomic analysis of healthy and diseased peri-implant microbiome under different periodontal conditions: a cross-sectional studySong, Liang, Feng, Ziying, Qianrong Zhou, Wu, Xingwen, Zhang, Limin, Yang Sun, Ruixue Li, Chen, Huijuan, Fei Yang, Yu, Youcheng · 2024 · Figshare
- Additional file 5 of Endometrial microbiota composition is associated with reproductive outcome in infertile patientsInmaculada Moreno, Iolanda Garcia-Grau, David Pérez-Villaroya, Marta Gonzáléz-Monfort, Bahçeci, Mustafa, Marcelo J. Barrionuevo, Sagiri Taguchi, Elena Puente, Michael DiMattina, Mei Wei Lim et al. · 2022 · Figshare
- Additional file 2 of Quantitative microbial population study reveals geographical differences in bacterial symbionts of Ixodes ricinusAleksandra I. Krawczyk, Lisa Röttjers, Manoj Fonville, Katsuhisa Takumi, Willem Takken, Karoline Faust, Hein Sprong · 2022 · Figshare
- Additional file 1 of Host reproductive cycle influences the pouch microbiota of wild southern hairy-nosed wombats (Lasiorhinus latifrons)Sesilje Weiss, Taggart, David, Ian Smith, Helgen, Kristofer M., Raphael Eisenhofer · 2021 · Figshare
- Additional file 3 of Fine scale transitions of the microbiota and metabolome along the gastrointestinal tract of herbivorous fishesWesley J. Sparagon, Emily C. Gentry, Jeremiah J. Minich, Lisa Vollbrecht, Lieve M. L. Laurens, Eric E. Allen, Neil Anthony Sims, Dorrestein, Pieter C., Linda Wegley Kelly, Nelson, Craig E. · 2022 · Figshare
- Additional file 1 of Signatures of landscape and captivity in the gut microbiota of Southern Hairy-nosed Wombats (Lasiorhinus latifrons)Raphael Eisenhofer, Helgen, Kristofer M., Taggart, David · 2021 · Figshare
- Additional file 1 of Microbial signatures and continuum in endometrial cancer and benign patientsAnita Semertzidou, Eilbhe Whelan, Ann Smith, Sherrianne Ng, Roberts, Lauren, Jan Joris Brosens, Marchesi, Julian R., Phillip R. Bennett, David A. MacIntyre, Kyrgiou, Maria · 2024 · Figshare
- Additional file 3 of The bacterial density of clinical rectal swabs is highly variable, correlates with sequencing contamination, and predicts patient risk of extraintestinal infectionRishi Chanderraj, Christopher A. Brown, Kevin J. Hinkle, Nicole R. Falkowski, Woods, Robert J., Dickson, Robert P. · 2022 · Figshare
- Additional file 2 of Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNAYacine Amar, Ilias Lagkouvardos, Silva, Rafaela L., Oluwaseun Ayodeji Ishola, Foesel, Bärbel U., Susanne Kublik, Anne Schöler, Sebastian Niedermeier, Rachela Bleuel, Alexander Zink et al. · 2021 · Figshare
- Additional file 2 of Multi-factorial examination of amplicon sequencing workflows from sample preparation to bioinformatic analysisTravis J. De Wolfe, Wright, Erik S. · 2023 · Figshare
- Additional file 1 of Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmonLi, Yanxian, Bruni, Leonardo, Alexander Jaramillo-Torres, Karina Gajardo, Trond M. Kortner, Åshild Krogdahl · 2021 · Figshare
- Additional file 1 of Peripheral blood mononuclear cells (PBMC) microbiome is not affected by colon microbiota in healthy goatsAinize Peña‐Cearra, Alejandro Belanche, Monika González-Lopez, Lavín, José Luis, Pascual-Itoiz, Miguel Ángel, Elisabeth Jiménez, Héctor Rodrı́guez, Aransay, Ana Mª., Juan Anguíta, Yáñez-Ruiz, David R. et al. · 2021 · Figshare
- Additional file 4 of Detection of cell-free microbial DNA using a contaminant-controlled analysis frameworkEnrique Zozaya‐Valdés, Stephen Q. Wong, Jeanette Raleigh, Athena Hatzimihalis, Sarah Ftouni, Papenfuss, Anthony T., Sandhu, Shahneen, Mark A. Dawson, Dawson, Sarah-Jane · 2021 · Figshare
- Additional file 1 of N/S element transformation modulating lithospheric microbial communities by single-species manipulationShun Yao, Tianzhi Jin, Zhang, Lu, Zhang, Yong, Chen, Rui, Qian Wang, Mingjie Lv, Chuxiao Hu, Ma, Ting, Xia, Wenjie · 2024 · Figshare
- Additional file 1 of Performance evaluation of high-volume electret filter air samplers in aerosol microbiome researchKari Oline Bøifot, Jostein Gohli, Gunnar Skogan, Marius Dybwad · 2020 · Figshare
- Additional file 11 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 2 of Dancing the Nanopore limbo – Nanopore metagenomics from small DNA quantities for bacterial genome reconstructionSophie A. Simon, Schmidt, Katharina, Lea Griesdorn, André Soares, Till L. V. Bornemann, Alexander J. Probst · 2024 · Figshare
- Additional file 3 of Signatures of landscape and captivity in the gut microbiota of Southern Hairy-nosed Wombats (Lasiorhinus latifrons)Raphael Eisenhofer, Helgen, Kristofer M., Taggart, David · 2021 · Figshare
- Additional file 4 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 5 of Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNAYacine Amar, Ilias Lagkouvardos, Silva, Rafaela L., Oluwaseun Ayodeji Ishola, Foesel, Bärbel U., Susanne Kublik, Anne Schöler, Sebastian Niedermeier, Rachela Bleuel, Alexander Zink et al. · 2021 · Figshare
- Additional file 1 of Human breast microbiome correlates with prognostic features and immunological signatures in breast cancerAlice Tzeng, Naseer Sangwan, Margaret R. Jia, Liu, Chin-Chih, Karen S. Keslar, Erinn P. Downs-Kelly, Robert L. Fairchild, Zahraa Al‐Hilli, Grobmyer, Stephen R., Charis Eng · 2021 · Figshare
- Additional file 1 of Dancing the Nanopore limbo – Nanopore metagenomics from small DNA quantities for bacterial genome reconstructionSophie A. Simon, Schmidt, Katharina, Lea Griesdorn, André Soares, Till L. V. Bornemann, Alexander J. Probst · 2024 · Figshare
- Additional file 3 of N/S element transformation modulating lithospheric microbial communities by single-species manipulationShun Yao, Tianzhi Jin, Zhang, Lu, Zhang, Yong, Chen, Rui, Qian Wang, Mingjie Lv, Chuxiao Hu, Ma, Ting, Wenjie Xia · 2024 · Figshare
- Additional file 2 of Peripheral blood mononuclear cells (PBMC) microbiome is not affected by colon microbiota in healthy goatsAinize Peña‐Cearra, Alejandro Belanche, Monika González-Lopez, Lavín, José Luis, Pascual-Itoiz, Miguel Ángel, Jiménez, Elisabeth, Héctor Rodrı́guez, Aransay, Ana Mª., Juan Anguíta, Yáñez-Ruiz, David R. et al. · 2021 · Figshare
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- Additional file 7 of Differences in airway microbiome and metabolome of single lung transplant recipientsSharma, Nirmal S., Vestal, Grant, Wille, Keith, Kapil Patel, Cheng, Feng, Srinivas M. Tipparaju, Sultan Tousif, Mudassir Meraj Banday, Xu, Xin, Wilson, Landon et al. · 2020 · Figshare
- Additional file 8 of Endometrial microbiota composition is associated with reproductive outcome in infertile patientsInmaculada Moreno, Iolanda Garcia-Grau, David Pérez-Villaroya, Marta Gonzáléz-Monfort, Bahçeci, Mustafa, Marcelo J. Barrionuevo, Sagiri Taguchi, Elena Puente, Michael DiMattina, Mei Wei Lim et al. · 2022 · Figshare
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- Additional file 1 of On the effect of inheritance of microbes in commensal microbiomesRomán Zapién-Campos, Florence Bansept, Michael Sieber, Arne Traulsen · 2022 · Figshare
- Additional file 7 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
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- Additional file 1 of Differences in airway microbiome and metabolome of single lung transplant recipientsSharma, Nirmal S., Vestal, Grant, Wille, Keith, Kapil Patel, Cheng, Feng, Srinivas M. Tipparaju, Sultan Tousif, Mudassir Meraj Banday, Xu, Xin, Wilson, Landon et al. · 2020 · Figshare
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- Additional file 9 of Differences in airway microbiome and metabolome of single lung transplant recipientsSharma, Nirmal S., Vestal, Grant, Wille, Keith, Kapil Patel, Cheng, Feng, Srinivas M. Tipparaju, Sultan Tousif, Banday, Mudassir M., Xu, Xin, Landon Wilson et al. · 2020 · Figshare
- Additional file 9 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 5 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 6 of Endometrial microbiota composition is associated with reproductive outcome in infertile patientsInmaculada Moreno, Iolanda Garcia-Grau, David Pérez-Villaroya, Marta Gonzáléz-Monfort, Bahçeci, Mustafa, Marcelo J. Barrionuevo, Sagiri Taguchi, Elena Puente, Michael DiMattina, Mei Wei Lim et al. · 2022 · Figshare
- Additional file 7 of Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNAYacine Amar, Ilias Lagkouvardos, Silva, Rafaela L., Oluwaseun Ayodeji Ishola, Foesel, Bärbel U., Susanne Kublik, Anne Schöler, Sebastian Niedermeier, Rachela Bleuel, Zink, Alexander et al. · 2021 · Figshare
- Additional file 2 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 3 of Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individualsJanis R. Bedarf, Naiara Beraza, Hassan Khazneh, Ezgi Özkurt, Baker, David, Borger, Valeri, Wüllner, Ullrich, Hildebrand, Falk · 2021 · Figshare
- Additional file 7 of Endometrial microbiota composition is associated with reproductive outcome in infertile patientsInmaculada Moreno, Iolanda Garcia-Grau, David Pérez-Villaroya, Marta Gonzáléz-Monfort, Bahçeci, Mustafa, Marcelo J. Barrionuevo, Sagiri Taguchi, Elena Puente, Michael DiMattina, Mei Wei Lim et al. · 2022 · Figshare
- Additional file 2 of The bacterial density of clinical rectal swabs is highly variable, correlates with sequencing contamination, and predicts patient risk of extraintestinal infectionRishi Chanderraj, Christopher A. Brown, Kevin J. Hinkle, Nicole R. Falkowski, Woods, Robert J., Dickson, Robert P. · 2022 · Figshare
- Additional file 1 of Exploring breast tissue microbial composition and the association with breast cancer risk factorsRana German, Natascia Marino, Christopher M. Hemmerich, Ram Podicheti, Douglas B. Rusch, Leah T. Stiemsma, Hongyu Gao, Xiaoling Xuei, Pam Rockey, Anna Maria Storniolo · 2024 · Figshare
- Additional file 3 of Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNAYacine Amar, Ilias Lagkouvardos, Silva, Rafaela L., Oluwaseun Ayodeji Ishola, Foesel, Bärbel U., Susanne Kublik, Anne Schöler, Sebastian Niedermeier, Rachela Bleuel, Zink, Alexander et al. · 2021 · Figshare
- Additional file 1 of Biological observations in microbiota analysis are robust to the choice of 16S rRNA gene sequencing processing algorithm: case study on human milk microbiotaShirin Moossavi, Faisal Atakora, Kelsey B. Fehr, Khafipour, Ehsan · 2020 · Figshare
- Additional file 3 of Differences in airway microbiome and metabolome of single lung transplant recipientsSharma, Nirmal S., Vestal, Grant, Wille, Keith, Kapil Patel, Cheng, Feng, Srinivas M. Tipparaju, Sultan Tousif, Mudassir Meraj Banday, Xu, Xin, Wilson, Landon et al. · 2020 · Figshare
- Additional file 1 of Detection of cell-free microbial DNA using a contaminant-controlled analysis frameworkEnrique Zozaya‐Valdés, Stephen Q. Wong, Jeanette Raleigh, Athena Hatzimihalis, Sarah Ftouni, Papenfuss, Anthony T., Sandhu, Shahneen, Mark A. Dawson, Dawson, Sarah-Jane · 2021 · Figshare
- Additional file 2 of Signatures of landscape and captivity in the gut microbiota of Southern Hairy-nosed Wombats (Lasiorhinus latifrons)Raphael Eisenhofer, Helgen, Kristofer M., Taggart, David · 2021 · Figshare
- Additional file 2 of Exploring breast tissue microbial composition and the association with breast cancer risk factorsRana German, Natascia Marino, Christopher M. Hemmerich, Ram Podicheti, Douglas B. Rusch, Leah T. Stiemsma, Hongyu Gao, Xiaoling Xuei, Pam Rockey, Anna Maria Storniolo · 2024 · Figshare
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