The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation
Abstract
Recent studies suggest that endocrine disrupting chemicals (EDCs) may form a risk factor for obesity by altering energy metabolism through epigenetic gene regulation. The goal of this study is to investigate the effects of a range of EDCs with putative obesogenic properties on global DNA methylation and adipocyte differentiation in vitro. Murine N2A and human SK-N-AS neuroblastoma cells and murine preadipocyte fibroblasts (3T3-L1) were exposed to tributyltin (TBT), diethylstilbestrol (DES), bisphenol A (BPA), 2,3,7,8-tetrachlorodibenzo-[p]-dioxin (TCDD), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153), hexachlorobenzene (HCB), hexabromocyclododecane (HBCD), 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) , perfluorinated octyl acid (PFOA) and perfluorinated octyl sulfonate (PFOS). A modest decrease in global DNA methylation was observed in N2A cells exposed to 10 μM DES, BPA, TCDD, BDE-47, PCB-153 and 1 μM HCB, but no changes were found in the human SK-N-AS cells. We reveal for the first time that BDE-47 increases adipocyte differentiation in a dose-dependent manner (2.5-25 μM). Adipocyte differentiation was also enhanced by TBT (≥ 10 nM) and BPA (>10 μM) and inhibited by TCDD (≥ 0.1 nM). The other chemicals showed either modest or no effects on adipocyte differentiation at the concentrations tested (PFOA, PFOS and HBCD at 10 μM; PCB-153, 3.4 μM and HCB, 1 μM). This study demonstrates that selected EDCs can induce functional changes in murine adipocyte differentiation in vitro which are accompanied by decreased global DNA methylation.
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“However, the latter finding may be unique to mouse cell lines since the DNA hypomethylating effects of PCB 153 were not observed in the human SK-N-AS neuroblastoma cell line [ 31 ].”
From DNA methylation: a mechanism linking environmental chemical exposures to risk of autism spectrum disorders? (2016) · cites this paper as [31]
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“Exposure to the NDL congener PCB 153 decreases DNMT activity in preimplantation mouse blastocytes [ 30 ], and decreases global DNA methylation levels in the N2A murine neuroblastoma cell line [ 31 ].”
From DNA methylation: a mechanism linking environmental chemical exposures to risk of autism spectrum disorders? (2016) · cites this paper as [31]
Citations
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Epigenetic Gene Promoter Methylation at Birth Is Associated With Child’s Later Adiposity
Godfrey, K. M., Sheppard, A. M., Gluckman, P. D., Lillycrop, K. A., Burdge, G. C., McLean, C. A., Rodford, J., Slater-Jefferies, J. L., Garratt, E. S., Crozier, S., et al. (2011) · Diabetes · doi:10.2337/db10-0979 · Cited by 761
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Early-life exposure to EDCs: role in childhood obesity and neurodevelopment
Braun, J. M. (2016) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2016.186 · Cited by 965
Obesity Pathogenesis: An Endocrine Society Scientific Statement
Schwartz, M. W., Seeley, R. J., Zeltser, L. M., Drewnowski, A., Ravussin, E., Redman, L. M., & Leibel, R. L. (2017) · Endocrine Reviews · doi:10.1210/er.2017-00111 · Cited by 727
Potential Mechanisms of Bisphenol A (BPA) Contributing to Human Disease
Cimmino, I., Fiory, F., Perruolo, G., Miele, C., Bèguinot, F., Formisano, P., & Oriente, F. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21165761 · Cited by 508
Endocrine disruptors and obesity
Heindel, J. J., Newbold, R., & Schug, T. T. (2015) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2015.163 · Cited by 451
Prenatal perfluoroalkyl substance exposure and child adiposity at 8 years of age: The HOME study
Braun, J. M., Chen, A., Romano, M. E., Calafat, A. M., Webster, G. M., Yolton, K. A., & Lanphear, B. P. (2015) · Obesity · doi:10.1002/oby.21258 · Cited by 249
Metadata
- Authors
- Bastos Sales, L., Kamstra, J. H., Cenijn, P. H., van Rijt, L. S., Hamers, T., & Legler, J.
- Affiliations
- Vrije Universiteit Amsterdam (NL)
- Corresponding author
- Legler, J.
- Venue
- Toxicology in Vitro
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 27
- Issue
- 6
- Pages
- 1634-1643
- ISSN
- 0887-2333 (ISSN-L)
- OpenAlex
- W2015008373
- Cited by
- 181
- References
- 48
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 27 · 2023: 7 · 2022: 13 · 2021: 14 · 2020: 14 · 2019: 10 · 2018: 21 · 2017: 18 · 2016: 15 · 2015: 10 · 2014: 23 · 2013: 1
- FWCI
- 11.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Toxicology in Vitro (publishedVersion)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- VU Research Portal (submittedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Keywords
- endocrine disruptors, global DNA methylation, adipogenesis, obesity, epigenetic regulation, bisphenol A, diethylstilbestrol, tributyltin, 2,3,7,8-tetrachlorodibenzo-p-dioxin, BDE-47, hexabromocyclododecane, PCB 153, hexachlorobenzene
- MeSH terms
- Bisphenol A Compounds, Animals, Benzhydryl Compounds, Caprylates, Cell Differentiation, Diethylstilbestrol, Environmental Pollutants, Fluorocarbons, Humans, Hydrocarbons, Brominated, Hydrocarbons, Chlorinated, Phenols, Trialkyltin Compounds, Adipocytes, Alkanesulfonic Acids, DNA Methylation, 3T3-L1 Cells, Cell Line, Tumor, Mice, Endocrine Disruptors, Halogenated Diphenyl Ethers
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