The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Cell membrane damage and protein interaction induced by copper containing nanoparticles—Importance of the metal release process
Open accessAbstract
Cu-containing nanoparticles are used in various applications in order to e.g. achieve antimicrobial activities and to increase the conductivity of fluids and polymers. Several studies have reported on toxic effects of such particles but the mechanisms are not completely clear. The aim of this study was to investigate the interactions between cell membranes and well-characterized nanoparticles of CuO, Cu metal, a binary Cu-Zn alloy and micron-sized Cu metal particles. This was conducted via in vitro investigations of the effects of the nanoparticles on (i) cell membrane damage on lung epithelial cells (A549), (ii) membrane rupture of red blood cells (hemolysis), complemented by (iii) nanoparticle interaction studies with a model lipid membrane using quartz crystal microbalance with dissipation monitoring (QCM-D). The results revealed that nanoparticles of the Cu metal and the Cu-Zn alloy were both highly membrane damaging and caused a rapid (within 1h) increase in membrane damage at a particle mass dose of 20 μg/mL, whereas the CuO nanoparticles and the micron-sized Cu metal particles showed no such effect. At similar nanoparticle surface area doses, the nano and micron-sized Cu particles showed more similar effects. The commonly used LDH (lactate dehydrogenase) assay for analysis of membrane damage was found impossible to use due to nanoparticle-assay interactions. None of the particles induced any hemolytic effects on red blood cells when investigated up to high particle concentrations (1mg/mL). However, both Cu and Cu-Zn nanoparticles caused hemoglobin aggregation/precipitation, a process that would conceal a possible hemolytic effect. Studies on interactions between the nanoparticles and a model membrane using QCM-D indicated a small difference between the investigated particles. Results of this study suggest that the observed membrane damage is caused by the metal release process at the cell membrane surface and highlight differences in reactivity between metallic nanoparticles of Cu and Cu-Zn and nanoparticles of CuO.
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Citations
Cites
Size matters: why nanomaterials are different
Roduner, E. (2006) · Chemical Society Reviews · doi:10.1039/b502142c · Cited by 2,317
Characterisation of copper oxide nanoparticles for antimicrobial applications
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Self-citationSurface Specific Kinetics of Lipid Vesicle Adsorption Measured with a Quartz Crystal Microbalance
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Acute toxicological effects of copper nanoparticles in vivo
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Size-dependent toxicity of metal oxide particles—A comparison between nano- and micrometer size
Karlsson, H. L., Gustafsson, J., Cronholm, P., & Möller, L. (2009) · Toxicology Letters · doi:10.1016/j.toxlet.2009.03.014 · Cited by 949
Self-citationLin, Y.-S., & Haynes, C. L. (2010) · Journal of the American Chemical Society · doi:10.1021/ja910846q · Cited by 837
Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae
Kasemets, K., Ivask, A., Dubourguier, H.-C., & Kahru, A. (2009) · Toxicology in Vitro · doi:10.1016/j.tiv.2009.05.015 · Cited by 627
The Use of Nanoparticles to Control Oral Biofilm Formation
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Cited by
Antimicrobial Nano-Agents: The Copper Age
Ermini, M. L., & Voliani, V. (2021) · ACS Nano · doi:10.1021/acsnano.0c10756 · Cited by 477
Nanotechnology in food science: Functionality, applicability, and safety assessment
He, X., & Hwang, H.-M. (2016) · Journal of Food and Drug Analysis · doi:10.1016/j.jfda.2016.06.001 · Cited by 474
Methods of Synthesis, Properties and Biomedical Applications of CuO Nanoparticles
Grigore, M., Biscu, E., Holban, A., Gestal, M., & Grumezescu, A. (2016) · Pharmaceuticals · doi:10.3390/ph9040075 · Cited by 445
Antimicrobial applications of copper
Vincent, M., Hartemann, P., & Engels-Deutsch, M. (2016) · International Journal of Hygiene and Environmental Health · doi:10.1016/j.ijheh.2016.06.003 · Cited by 384
Ma, X., Zhou, S., Xu, X., & Du, Q. (2022) · Frontiers in Surgery · doi:10.3389/fsurg.2022.905892 · Cited by 317
Metadata
- Authors
- Karlsson, H. L., Cronholm, P., Hedberg, Y., Tornberg, M., De Battice, L., Svedhem, S., & Wallinder, I. O.
- Venue
- Toxicology
- Publisher
- Elsevier BV
- Published
- 2013-11
- Volume
- 313
- Issue
- 1
- Pages
- 59-69
- ISSN
- 0300-483X (ISSN-L)
- OpenAlex
- W2137260452
- Cited by
- 286
- References
- 31
- FWCI
- 9.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Karlsson, H. L., Cronholm, P., Hedberg, Y., Tornberg, M., De Battice, L., Svedhem, S., & Wallinder, I. O. (2013). Cell membrane damage and protein interaction induced by copper containing nanoparticles—Importance of the metal release process. Toxicology. https://doi.org/10.1016/j.tox.2013.07.012
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