UVB-induced apoptosis and DNA damaging potential of chrysene via reactive oxygen species in human keratinocytes
Abstract
Chrysene is one of the basic polycyclic aromatic hydrocarbon (PAH) which is toxic environmental pollutant and consistently exposed to sunlight. However, little information is available on its photogenotoxicity. The objective of the present study was to analyze the effects of chrysene, under environmental intensity of UVB (0.6mW/cm(2)) in human skin epidermal cell line (HaCaT). Kinetic of chrysene showed that the highest intracellular uptake of chrysene occurred after 24h of incubation. The intracellular reactive oxygen species (ROS) was increased in a concentration dependent manner in chrysene treated cells under UVB irradiation. It was observed that UVB-irradiated chrysene induced apoptosis through activation of caspases-3 and phosphatidylserine translocation. Glutathione reduced (GSH) and catalase activity were decreased while apoptosis and DNA damage were induced significantly (P>0.01) as concentration of chrysene increased. Thus our results suggest that chrysene may be phototoxic as well as photogenotoxic under UVB irradiation.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
DNA damage as photogenotoxicity can be found under UV-B irradiation.
“Literature also reported that DNA damage as photogenotoxicity can be found under UV-B irradiation”
From Skin protection from solar ultraviolet radiation using natural compounds: a review (2024) · cites this paper as Ali et al. 2011
Cites
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., & Fusenig, N. E. (1988) · The Journal of cell biology · doi:10.1083/jcb.106.3.761 · Cited by 4,170 · see citing works
The Comet Assay for DNA Damage and Repair: Principles, Applications, and Limitations
Collins, A. R. (2004) · Molecular Biotechnology · doi:10.1385/mb:26:3:249 · Cited by 2,892 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Lucas, R. M., Norval, M., Neale, R. E., Young, A. R., de Gruijl, F. R., Takizawa, Y., & van der Leun, J. C. (2014) · Photochemical & Photobiological Sciences · doi:10.1039/c4pp90033b · Cited by 158 · see citing works
Skin protection from solar ultraviolet radiation using natural compounds: a review
Verma, A., Zanoletti, A., Kareem, K. Y., Adelodun, B., Kumar, P., Ajibade, F. O., Silva, L. F. O., Phillips, A. J., Kartheeswaran, T., Bontempi, E., et al. (2024) · Environmental Chemistry Letters · doi:10.1007/s10311-023-01649-4 · Cited by 102 · see citing works
Self-citationSrivastav, A. K., Agnihotry, S., Mujtaba, S. F., Negi, S., Verma, A., & Ray, R. S. (2018) · Photocarcinogenesis & Photoprotection · doi:10.1007/978-981-10-5493-8_9 · Cited by 5 · see citing works
Self-citation
- Authors
- Ali, D., Verma, A., Mujtaba, F., Dwivedi, A., Hans, R. K., & Ray, R. S.
- Affiliations
- Indian Institute of Toxicology Research (IN)
- Corresponding author
- Ray, R.S.
- Venue
- Toxicology Letters
- Publisher
- Elsevier BV
- Published
- 2011-07
- Volume
- 204
- Issue
- 2-3
- Pages
- 199-207
- ISSN
- 0378-4274 (ISSN-L)
- OpenAlex
- W2061625453
- Cited by
- 64
- References
- 2
- Citations by year
- 2025: 5 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 3 · 2017: 4 · 2016: 8 · 2015: 3 · 2014: 8 · 2013: 4 · 2012: 2
- FWCI
- 1.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Toxicology Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- IITR
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- chrysene, UVB irradiation, phototoxicity, photogenotoxicity, reactive oxygen species, oxidative stress, apoptosis, caspase-3, phosphatidylserine translocation, DNA damage, polycyclic aromatic hydrocarbons, HaCaT keratinocytes, glutathione, catalase, cellular uptake
- MeSH terms
- Catalase, Cell Survival, Cells, Cultured, Chrysenes, DNA Damage, Glutathione, Humans, Phosphatidylserines, Ultraviolet Rays, Keratinocytes, Apoptosis, Annexin A5, Reactive Oxygen Species, Caspase 3
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
