Pretreatment of garlic powder using sweep frequency ultrasound and single frequency countercurrent ultrasound: Optimization and comparison for ACE inhibitory activities
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The sweep frequency ultrasound (SFU) and single frequency countercurrent ultrasound (SFCU) pretreatments were modeled and compared based on production of angiotensin I-converting enzyme (ACE) inhibitory peptides from garlic hydrolysates. Two mathematical models were developed to show the effect of each variable and their combinatorial interactions on ACE inhibitory activity. The optimum levels of the parameters in SFU were determined using uniform design, which revealed these as follows: total ultrasonic time 1.5 h, on-time of pulse 18 s and off-time of pulse 3 s. Under optimized conditions, the experimental values of SFU and SFCU were 65.88% and 67.78%, which agreed closely with the predicted values of 63.44% and 67.33%. The SFU and SFCU pretreatments both resulted in higher ACE inhibitory activity compared with untreated garlic (p<0.05). However, there were no significant differences in the ACE inhibitory activities and IC₅₀ values obtained from SFCU and SFU pretreatments under optimum conditions (p>0.05).
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SFU and SFCU pretreatments both led to significantly increased ACE inhibitory activity in garlic powder compared to untreated garlic.
“The application of SFU and SFCU pretreatments both led to significantly increased ACE inhibitory activity in garlic powder compared to untreated garlic.”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [107]
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- Authors
- Ma, H., Huang, L., Peng, L., Wang, Z., & Yang, Q.
- Affiliations
- Jiangsu University (CN)
- Corresponding author
- Ma, Haile
- Venue
- Ultrasonics Sonochemistry
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 23
- Pages
- 109-115
- ISSN
- 1350-4177 (ISSN-L)
- OpenAlex
- W2025078316
- Cited by
- 40
- References
- 30
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 7 · 2022: 1 · 2021: 6 · 2020: 7 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 1
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Ultrasonics Sonochemistry (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Innovation Fund for Jiangsu Agricultural Science and Technology (CX(12)3084)
- Jiangsu Provincial Technology Support Program—Agricultural Part (BE2012393)
- Program of Jiangsu Provincial Six Big Talents Summit
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Physical Sciences › Materials Science › Materials Chemistry › Ultrasound and Cavitation Phenomena
- Keywords
- garlic powder, angiotensin-converting enzyme inhibition, ACE inhibitory peptides, enzymatic hydrolysate, ultrasonic pretreatment, process optimization, uniform design, mathematical modeling, IC50
- MeSH terms
- Angiotensin-Converting Enzyme Inhibitors, Garlic, Plant Extracts, Powders, Ultrasonics
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