Cited by 40Garlic and Onion StudiesProtein Hydrolysis and Bioactive PeptidesUltrasound and Cavitation Phenomena

Pretreatment of garlic powder using sweep frequency ultrasound and single frequency countercurrent ultrasound: Optimization and comparison for ACE inhibitory activities

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Ma, Haile, Huang, Liurong, Peng, Lei, Wang, Zhenbin, Yang, Qiaorong·2015·Ultrasonics Sonochemistry

Abstract

The sweep frequency ultrasound (SFU) and single frequency countercurrent ultrasound (SFCU) pretreatments were modeled and compared based on production of angiotensin I-converting enzyme (ACE) inhibitory peptides from garlic hydrolysates. Two mathematical models were developed to show the effect of each variable and their combinatorial interactions on ACE inhibitory activity. The optimum levels of the parameters in SFU were determined using uniform design, which revealed these as follows: total ultrasonic time 1.5 h, on-time of pulse 18 s and off-time of pulse 3 s. Under optimized conditions, the experimental values of SFU and SFCU were 65.88% and 67.78%, which agreed closely with the predicted values of 63.44% and 67.33%. The SFU and SFCU pretreatments both resulted in higher ACE inhibitory activity compared with untreated garlic (p<0.05). However, there were no significant differences in the ACE inhibitory activities and IC₅₀ values obtained from SFCU and SFU pretreatments under optimum conditions (p>0.05).

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  1. L. Peng, H. Ma, R. He, W. Qu, L. Luo, Trans. CSAE 28 (2012) 333-338.
  2. Q.Y. Wu, Ph.D. Thesis of Jiangsu University, Jiangsu, China, 2004.

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Authors
Ma, H., Huang, L., Peng, L., Wang, Z., & Yang, Q.
Affiliations
Jiangsu University (CN)
Corresponding author
Ma, Haile
Venue
Ultrasonics Sonochemistry
Publisher
Elsevier BV
Published
2015-03
Volume
23
Pages
109-115
ISSN
1350-4177 (ISSN-L)
OpenAlex
W2025078316
Cited by
40
References
30
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 7 · 2022: 1 · 2021: 6 · 2020: 7 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 1
FWCI
3.11
Type
journal article
Language
en
Open access
Locations
  • Ultrasonics Sonochemistry (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Innovation Fund for Jiangsu Agricultural Science and Technology (CX(12)3084)
  • Jiangsu Provincial Technology Support Program—Agricultural Part (BE2012393)
  • Program of Jiangsu Provincial Six Big Talents Summit
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Physical Sciences › Materials Science › Materials Chemistry › Ultrasound and Cavitation Phenomena
Keywords
garlic powder, angiotensin-converting enzyme inhibition, ACE inhibitory peptides, enzymatic hydrolysate, ultrasonic pretreatment, process optimization, uniform design, mathematical modeling, IC50
MeSH terms
Angiotensin-Converting Enzyme Inhibitors, Garlic, Plant Extracts, Powders, Ultrasonics

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