Cited by 130Tuberculosis Research and EpidemiologyImmunotherapy and Immune ResponsesImmune responses and vaccinations

Nanoparticle conjugation and pulmonary delivery enhance the protective efficacy of Ag85B and CpG against tuberculosis

Open access
Ballester, Marie, Nembrini, Chiara, Dhar, Neeraj, de Titta, Alexandre, de Piano, Cyntia, Pasquier, Miriella, Simeoni, Eleonora, van der Vlies, André J. +3 more·2011·Vaccine

Abstract

Vaccines that drive robust T-cell immunity against Mycobacterium tuberculosis (Mtb) are needed both for prophylactic and therapeutic purposes. We have recently developed a synthetic vaccine delivery platform with Pluronic-stabilized polypropylene sulfide nanoparticles (NPs), which target lymphoid tissues by their small size (∼ 30 nm) and which activate the complement cascade by their surface chemistry. Here we conjugated the tuberculosis antigen Ag85B to the NPs (NP-Ag85B) and compared their efficacy in eliciting relevant immune responses in mice after intradermal or pulmonary administration. Pulmonary administration of NP-Ag85B with the adjuvant CpG led to enhanced induction of antigen-specific polyfunctional Th1 responses in the spleen, the lung and lung-draining lymph nodes as compared to soluble Ag85B with CpG and to the intradermally-delivered formulations. Mucosal and systemic Th17 responses were also observed with this adjuvanted NP formulation and vaccination route, especially in the lung. We then evaluated protection induced by the adjuvanted NP formulation following a Mtb aerosol challenge and found that vaccination with NP-Ag85B and CpG via the pulmonary route displayed a substantial reduction of the lung bacterial burden, both compared to soluble Ag85B with CpG and to the corresponding intradermally delivered formulations. These findings highlight the potential of administrating NP-based formulations by the pulmonary route for TB vaccination.

Integrity signals

Registry

Crossref · 10.1016/j.vaccine.2011.07.039

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Pulmonary administration of the common TB antigen 85B conjugated to poly(propylene sulphide) nanoparticles demonstrated improved protection in mice after challenge with aerosolized M. tuberculosis.

    “Pulmonary administration of the common TB antigen 85B conjugated to poly(propylene sulphide) nanoparticles demonstrated improved protection in mice after they were challenged with aerosolized M. tuberculosis (ref. 135 ).”

    From Nanotechnology approaches for global infectious diseases (2021) · cites this paper as [135]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Ballester, M., Nembrini, C., Dhar, N., de Titta, A., de Piano, C., Pasquier, M., Simeoni, E., van der Vlies, A. J., McKinney, J. D., Hubbell, J. A., & Swartz, M. A.
Affiliations
École Polytechnique Fédérale de Lausanne (CH)
Corresponding author
  • Hubbell, Jeffrey A.
  • Swartz, Melody A.
Venue
Vaccine
Publisher
Elsevier BV
Published
2011-09
Volume
29
Issue
40
Pages
6959-6966
ISSN
0264-410X (ISSN-L)
OpenAlex
W1967877494
Cited by
130
References
33
Citations by year
2026: 3 · 2025: 5 · 2024: 8 · 2023: 12 · 2022: 1 · 2021: 9 · 2020: 6 · 2019: 8 · 2018: 13 · 2017: 9 · 2016: 7 · 2015: 7 · 2014: 15 · 2013: 16 · 2012: 10
FWCI
8.85
Type
journal article
Language
en
Open access
Locations
  • Vaccine (publishedVersion)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Funders
Bill and Melinda Gates Foundation
Topics
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
Keywords
tuberculosis vaccination, Ag85B, CpG adjuvant, nanoparticle conjugation, Th1 response, Th17 response, Mycobacterium tuberculosis, aerosol challenge, lung bacterial burden, complement activation
MeSH terms
Acyltransferases, Animals, Antigens, Bacterial, Bacterial Proteins, Complement System Proteins, Dendritic Cells, Lung, Lymph Nodes, Mice, Inbred C57BL, Mycobacterium tuberculosis, Particle Size, Polypropylenes, Spleen, Tuberculosis, Vaccines, Synthetic, Th1 Cells, Immunoconjugates, CpG Islands, Tuberculosis Vaccines, Mice, Nanoparticles, Th17 Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review