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Cited by 49Parasitic Infections and DiagnosticsAmoebic Infections and TreatmentsViral gastroenteritis research and epidemiology

α1-giardin based live heterologous vaccine protects against Giardia lamblia infection in a murine model

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Jenikova, Gabriela, Hruz, Petr, Andersson, Mattias K., Tejman-Yarden, Noa, Ferreira, Patricia C.D., Andersen, Yolanda S., Davids, Barbara J., Gillin, Frances D. +3 more·2011·Vaccine
DOI 10.1016/j.vaccine.2011.09.126PMC PMC4045459
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Abstract

Giardia lamblia is a leading protozoan cause of diarrheal disease worldwide, yet preventive medical strategies are not available. A crude veterinary vaccine has been licensed for cats and dogs, but no defined human vaccine is available. We tested the vaccine potential of three conserved antigens previously identified in human and murine giardiasis, α1-giardin, α-enolase, and ornithine carbamoyl transferase, in a murine model of G. lamblia infection. Live recombinant attenuated Salmonella enterica Serovar Typhimurium vaccine strains were constructed that stably expressed each antigen, maintained colonization capacity, and sustained total attenuation in the host. Oral administration of the vaccine strains induced antigen-specific serum IgG, particularly IgG(2A), and mucosal IgA for α1-giardin and α-enolase, but not for ornithine carbamoyl transferase. Immunization with the α1-giardin vaccine induced significant protection against subsequent G. lamblia challenge, which was further enhanced by boosting with cholera toxin or sublingual α1-giardin administration. The α-enolase vaccine afforded no protection. Analysis of α1-giardin from divergent assemblage A and B isolates of G. lamblia revealed >97% amino acid sequence conservation and immunological cross-reactivity, further supporting the potential utility of this antigen in vaccine development. Together. These results indicate that α1-giardin is a suitable candidate antigen for a vaccine against giardiasis.

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  • Immunization with a live, recombinant alpha-1 giardin vaccine formulation provided protection from a Giardia challenge in a murine model.

    “immunization with a live, recombinant alpha-1 giardin vaccine formulation provided protection from a Giardia challenge in a murine model (8).”

    From Comparative Cell Biology and Evolution of Annexins in Diplomonads (2016) · cites this paper as [8]

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Authors
Jenikova, G., Hruz, P., Andersson, M. K., Tejman-Yarden, N., Ferreira, P. C. D., Andersen, Y. S., Davids, B. J., Gillin, F. D., Svärd, S. G., Curtiss, R., & Eckmann, L.
Affiliations
  • University of California San Diego (US)
  • Uppsala University (SE)
  • Arizona State University (US)
Corresponding author
Eckmann, Lars
ORCID
Venue
Vaccine
Publisher
Elsevier BV
Published
2011-11
Volume
29
Issue
51
Pages
9529-9537
ISSN
0264-410X (ISSN-L)
DOI
10.1016/j.vaccine.2011.09.126
PMC
PMC4045459
OpenAlex
W2073178782
Cited by
49
References
1
Citations by year
2026: 2 · 2024: 2 · 2023: 3 · 2022: 6 · 2021: 3 · 2020: 2 · 2019: 6 · 2017: 2 · 2016: 4 · 2015: 8 · 2014: 3 · 2013: 4 · 2012: 4
FWCI
2.47
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Vaccine (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • NIH (DK35108, DK80506, AI60557, AI56289)
  • Swedish Research Council
  • Sweden-America Foundation
  • FAPESP (São Paulo Reasearch Foundation)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
  • Health Sciences › Medicine › Infectious Diseases › Amoebic Infections and Treatments
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
Keywords
Giardia lamblia, giardiasis, live attenuated vaccine, alpha-enolase, ornithine carbamoyltransferase, vaccine candidate antigens, mucosal IgA, sublingual immunization, immunological cross-reactivity, mouse model
MeSH terms
Administration, Oral, Animals, Antibodies, Protozoan, Antigens, Protozoan, Cholera Toxin, Cytoskeletal Proteins, Giardiasis, Mice, Inbred BALB C, Ornithine Carbamoyltransferase, Phosphopyruvate Hydratase, Salmonella typhimurium, Vaccines, Synthetic, Protozoan Proteins, Protozoan Vaccines, Giardia lamblia, Mice

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