Protection against herpes simplex virus type 2 infection in a neonatal murine model using a trivalent nucleoside-modified mRNA in lipid nanoparticle vaccine
Open accessAbstract
Neonatal herpes is a dreaded complication of genital herpes infection in pregnancy. We recently compared two vaccine platforms for preventing genital herpes in female mice and guinea pigs and determined that HSV-2 glycoproteins C, D and E expressed using nucleoside-modified mRNA in lipid nanoparticles provided better protection than the same antigens produced as baculovirus proteins and administered with CpG and alum. Here we evaluated mRNA and protein immunization for protection against neonatal herpes. Female mice were immunized prior to mating and newborns were infected intranasally with HSV-2. IgG binding and neutralizing antibody levels in mothers and newborns were comparable using the mRNA or protein vaccines. Both vaccines protected first and second litter newborns against disseminated infection based on virus titers in multiple organs. We conclude that both vaccines are efficacious at preventing neonatal herpes, which leaves the mRNA vaccine as our preferred candidate based on better protection against genital herpes.
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In several studies, maternal vaccination with mRNA-loaded LNPs protected mouse neonates from herpesvirus.
“In several studies, maternal vaccination with mRNA-loaded LNPs prevented fetal Zika virus transmission in pregnant mice225,226 and protected mouse neonates from herpesvirus227 and group A and group B streptococci228.”
From mRNA vaccines for infectious diseases: principles, delivery and clinical translation (2021) · cites this paper as [227]
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mRNA vaccines for infectious diseases: principles, delivery and clinical translation
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- Authors
- LaTourette, P. C., Awasthi, S., Desmond, A., Pardi, N., Cohen, G. H., Weissman, D., & Friedman, H. M.
- Affiliations
- University of Pennsylvania (US)
- Children's Hospital of Philadelphia (US)
- Corresponding author
- Friedman, Harvey M.
- Venue
- Vaccine
- Publisher
- Elsevier BV
- Published
- 2020-11
- Volume
- 38
- Issue
- 47
- Pages
- 7409-7413
- ISSN
- 0264-410X (ISSN-L)
- PMC
- PMC7545304
- OpenAlex
- W3092544928
- Cited by
- 39
- References
- 20
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 9 · 2023: 4 · 2022: 8 · 2021: 8 · 2020: 1
- FWCI
- 3.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Vaccine (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- herpes simplex virus type 2, neonatal herpes, mRNA vaccines, nucleoside-modified mRNA, lipid nanoparticles, maternal immunization, passive antibody transfer, disseminated infection, genital herpes in pregnancy
- MeSH terms
- Animals, Antibodies, Viral, Disease Models, Animal, Female, Guinea Pigs, Herpes Genitalis, Herpes Simplex, Lipids, Nucleosides, Pregnancy, RNA, Messenger, Vaccines, Viral Envelope Proteins, Herpesvirus 2, Human, Herpes Simplex Virus Vaccines, Mice, Nanoparticles
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