The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Reactivity of human sera in a sensitive, high-throughput pseudovirus-based papillomavirus neutralization assay for HPV16 and HPV18
Open accessAbstract
Sensitive high-throughput neutralization assays, based upon pseudoviruses carrying a secreted alkaline phosphatase (SEAP) reporter gene, were developed and validated for human papillomavirus (HPV)16, HPV18, and bovine papillomavirus 1 (BPV1). SEAP pseudoviruses were produced by transient transfection of codon-modified papillomavirus structural genes into an SV40 T antigen expressing line derived from 293 cells, yielding sufficient pseudovirus from one flask for thousands of titrations. In a 96-well plate format, in this initial characterization, the assay was reproducible and appears to be as sensitive as, but more specific than, a standard papillomavirus-like particle (VLP)-based enzyme-linked immunosorbent assay (ELISA). The neutralization assay detected type-specific HPV16 or HPV18 neutralizing antibodies (titers of 160-10240) in sera of the majority of a group of women infected with the corresponding HPV type, but not in virgin women. Sera from HPV16 VLP vaccinees had high anti-HPV16 neutralizing titers (mean: 45000; range: 5120-163840), but no anti-HPV18 neutralizing activity. The SEAP pseudovirus-based neutralization assay should be a practical method for quantifying potentially protective antibody responses in HPV natural history and prophylactic vaccine studies.
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Cites
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., & Fusenig, N. E. (1988) · The Journal of cell biology · doi:10.1083/jcb.106.3.761 · Cited by 4,170
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Correlates of protection induced by vaccination
Plotkin, S. A. (2010) · Clinical and Vaccine Immunology · doi:10.1128/cvi.00131-10 · Cited by 1,745
Carrageenan is a potent inhibitor of papillomavirus infection
Buck, C. B., Thompson, C. D., Roberts, J. N., Müller, M., Lowy, D. R., & Schiller, J. T. (2006) · PLoS Pathogens · doi:10.1371/journal.ppat.0020069 · Cited by 497
Self-citationNieto, K., Stahl-Hennig, C., Leuchs, B., Müller, M., Gissmann, L., & Kleinschmidt, J. A. (2012) · Human Gene Therapy · doi:10.1089/hum.2011.202 · Cited by 46
Metadata
- Authors
- Pastrana, D. V., Buck, C. B., Pang, Y.-Y. S., Thompson, C. D., Castle, P. E., FitzGerald, P. C., Krüger Kjaer, S., Lowy, D. R., & Schiller, J. T.
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2004-04
- Volume
- 321
- Issue
- 2
- Pages
- 205-216
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W2001429203
- Cited by
- 357
- References
- 1
- FWCI
- 11.71
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Pastrana, D. V., Buck, C. B., Pang, Y.-Y. S., Thompson, C. D., Castle, P. E., FitzGerald, P. C., Krüger Kjaer, S., Lowy, D. R., & Schiller, J. T. (2004). Reactivity of human sera in a sensitive, high-throughput pseudovirus-based papillomavirus neutralization assay for HPV16 and HPV18. Virology. https://doi.org/10.1016/j.virol.2003.12.027
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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