CoChat
Sign Up
Cited by 83Herpesvirus Infections and TreatmentsRNA and protein synthesis mechanismsCytomegalovirus and herpesvirus research

Proteomics of herpes simplex virus infected cell protein 27: association with translation initiation factors

Open access
Fontaine-Rodriguez, Errin C., Taylor, Travis J, Olesky, Melanie, Knipe, David M.·2004·Virology
DOI 10.1016/j.virol.2004.10.002
Open this paper in CoChat — free accountOpen PDF

Abstract

The herpes simplex virus (HSV) immediate early ICP27 protein plays an essential role in stimulating viral early and late gene expression. ICP27 appears to be multifunctional in that it has been reported to stimulate viral late gene transcription, polyadenylation site usage, and RNA export. We report here on proteomic studies involving immunoprecipitation of ICP27 and mass spectrometric identification of co-precipitated proteins. These studies show an association of ICP27 with the cellular translation initiation factors poly A binding protein (PABP), eukaryotic initiation factor 3 (eIF3), and eukaryotic initiation factor 4G (eIF4G) in infected cells. Immunoprecipitation-western blot studies confirmed these associations. Finally, purified MBP-tagged ICP27 (MBP-27) can interact with eIF3 subunits p47 and p116 in vitro. These results suggest that ICP27 may also play a role in stimulating translation of certain viral and host mRNAs and/or in inhibiting host mRNA translation.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.virol.2004.10.002

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Components of SWI/SNF and ISWI chromatin-remodeling complexes were coisolated with the single-stranded DNA-binding protein ICP8.

    “Interestingly, components of SWI/SNF and ISWI chromatin-remodeling complexes were coisolated with the single-stranded DNA–binding protein ICP8.”

    From The Impact of Mass Spectrometry–Based Proteomics on Fundamental Discoveries in Virology (2014) · cites this paper as [34]

  • The Knipe laboratory used affinity purification-mass spectrometry to study virus-host protein complexes containing ICP27 or ICP8 at an early stage of herpes simplex virus 1 infection in human Hep2 cells.

    “For example, the Knipe laboratory (34, 35) used AP-MS to study virus-host protein complexes containing the immediate-early proteins ICP27 or ICP8 at an early stage of herpes simplex virus 1 (HSV-1) infection in human Hep2 cells.”

    From The Impact of Mass Spectrometry–Based Proteomics on Fundamental Discoveries in Virology (2014) · cites this paper as [34]

  • In HSV-1-infected human epidermoid cells at 6 hpi, ICP27 interacts with eIF3 subunits p47 and p116, eIF4G, and poly-adenylate binding protein, suggesting ICP27 may interfere with viral or host mRNA translation.

    “the Knipe group subsequently identified the eukaryotic translation initiation eIF3 subunits p47 and p116, eIF4G, and poly-adenylate binding protein as cellular interactors of the HSV-1 immediate-early protein ICP27 in human epidermoid cells at 6 hpi. These results suggested that ICP27 may interfere with viral or host mRNA translation [36].”

    From Proteomic approaches to uncovering virus–host protein interactions during the progression of viral infection (2016) · cites this paper as [36]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. Roizman, B., Knipe, D.M., 2001. Herpes simplex viruses and their replication. In: Knipe, D.M., Howley, P.M. (Eds.), Fields Virology, 4th ed. Lippincott, Williams and Wilkins, Philadelphia, PA, pp. 2399 -2460.

Cited by

Sorted by citations

Related

Authors
Fontaine-Rodriguez, E. C., Taylor, T. J., Olesky, M., & Knipe, D. M.
Affiliations
Harvard University (US)
Corresponding author
Knipe, David M.
ORCID
Knipe, David M.
Venue
Virology
Publisher
Elsevier BV
Published
2004-12
Volume
330
Issue
2
Pages
487-492
ISSN
0042-6822 (ISSN-L)
DOI
10.1016/j.virol.2004.10.002
OpenAlex
W1983841815
Cited by
83
References
33
Citations by year
2025: 1 · 2024: 3 · 2022: 1 · 2021: 2 · 2020: 2 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 7 · 2014: 4 · 2013: 1 · 2012: 4
FWCI
2.88
Type
journal article
Language
en
Open access
Licence
Locations
  • Virology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Allergy and Infectious Diseases (AI-20530, R01AI020530, T32AI007245)
Topics
  • Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
Keywords
herpes simplex virus, ICP27, viral immediate-early proteins, translation initiation factors, poly(A)-binding protein, eIF3, eIF4G, protein-protein interaction, viral gene expression, viral mRNA translation
MeSH terms
Cell Line, Humans, Mass Spectrometry, Blotting, Western, Immediate-Early Proteins, Simplexvirus, Protein Subunits, Protein Interaction Mapping, Poly(A)-Binding Proteins, Eukaryotic Initiation Factor-4G, Eukaryotic Initiation Factor-3, Eukaryotic Initiation Factors, Immunoprecipitation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review