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The stability of the human papillomavirus E6 oncoprotein is E6AP dependent

Tomaić, Vjekoslav, Pim, David, Banks, Lawrence·2009·Virology
DOI 10.1016/j.virol.2009.07.029
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Abstract

Human papillomavirus (HPV) E6 oncoproteins target numerous cellular proteins for ubiquitin-mediated degradation. In the case of p53 this is mediated by the E6AP ubiquitin ligase. However, there are conflicting reports concerning how central E6AP is to the global function of the HPV-16 and HPV-18 E6 oncoproteins. To investigate this further we have analysed the effects of E6AP removal upon the stability of endogenously expressed E6 protein. We show that when E6AP is silenced in HPV-positive cells, E6 protein levels are dramatically decreased in a proteasome-dependent manner. Further, we show that when E6AP is depleted in HeLa cells, E6 has a greatly decreased half-life. In addition, overexpression of E6AP stabilises ectopically expressed HPV-16 and HPV-18 E6 in a manner that is independent of its ubiquitin ligase activity. These results demonstrate that the stability of HPV E6 is critically dependent upon the presence of E6AP.

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Research works printed but not linked: 25

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Metadata

Authors
Tomaić, V., Pim, D., & Banks, L.
Venue
Virology
Publisher
Elsevier BV
Published
2009-10
Volume
393
Issue
1
Pages
7-10
ISSN
0042-6822
DOI
10.1016/j.virol.2009.07.029
Cited by
76
References
0
Language
en
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CoChat. (2026). Citation check: The stability of the human papillomavirus E6 oncoprotein is E6AP dependent. https://app.cochat.ai/papers/10.1016/j.virol.2009.07.029
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Tomaić, V., Pim, D., & Banks, L. (2009). The stability of the human papillomavirus E6 oncoprotein is E6AP dependent. Virology. https://doi.org/10.1016/j.virol.2009.07.029