The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Protocol for Primary Mouse Hepatocyte Isolation
Open accessAbstract
Primary hepatocytes are a vital tool in various biomedical research disciplines, serving as an ex vivo model for liver physiology. Obtaining high yields of viable primary mouse hepatocytes is technically challenging, limiting their use. Here, we present an improved protocol based on the classic two-step collagenase perfusion technique. The liver is washed by perfusion, hepatocytes are dissociated by collagenase, separated from other cells, and cultured. This protocol was optimized to significantly reduce procedure duration and improve hepatocyte yield and viability.
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Cites
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HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS
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Mouse liver cell culture: I. Hepatocyte isolation
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Isolation and Culture of Adult Mouse Hepatocytes
Li, W.-C., Ralphs, K. L., & Tosh, D. (2010) · Methods in Molecular Biology · doi:10.1007/978-1-59745-019-5_13 · Cited by 298
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Cell-Type-Resolved Quantitative Proteomics of Murine Liver
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Transcription factor assisted loading and enhancer dynamics dictate the hepatic fasting response
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Self-citationp53 promotes the expression of gluconeogenesis-related genes and enhances hepatic glucose production
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Physiological β-amyloid clearance by the liver and its therapeutic potential for Alzheimer’s disease
Cheng, Y., He, C.-Y., Tian, D.-Y., Chen, S.-H., Ren, J.-R., Sun, H.-L., Xu, M.-Y., Tan, C.-R., Fan, D.-Y., Jian, J.-M., et al. (2023) · Acta Neuropathologica · doi:10.1007/s00401-023-02559-z · Cited by 110
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Roehlen, N., Saviano, A., El Saghire, H., Crouchet, E., Nehme, Z., Del Zompo, F., Jühling, F., Oudot, M. A., Durand, S. C., Duong, F. H. T., et al. (2022) · Science Translational Medicine · doi:10.1126/scitranslmed.abj4221 · Cited by 54
Marinović, S., Lenartić, M., Mladenić, K., Šestan, M., Kavazović, I., Benić, A., Krapić, M., Rindlisbacher, L., Cokarić Brdovčak, M., Sparano, C., et al. (2023) · Science Immunology · doi:10.1126/sciimmunol.add1599 · Cited by 43
Hepatocyte CYR61 polarizes profibrotic macrophages to orchestrate NASH fibrosis
Mooring, M., Yeung, G. A., Luukkonen, P., Liu, S., Akbar, M. W., Zhang, G. J., Balogun, O., Yu, X., Mo, R., Nejak-Bowen, K., et al. (2023) · Science Translational Medicine · doi:10.1126/scitranslmed.ade3157 · Cited by 33
Metadata
- Authors
- Charni-Natan, M., & Goldstein, I.
- Venue
- STAR Protocols
- Publisher
- Elsevier BV
- Published
- 2020-09
- Volume
- 1
- Issue
- 2
- Pages
- 100086
- ISSN
- 2666-1667 (ISSN-L)
- OpenAlex
- W3048486047
- Cited by
- 426
- References
- 13
- FWCI
- 15.26
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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