The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination
Open accessAbstract
Objectives Cell lines derived from human ovarian and endometrial cancers, and their immortalized non-malignant counterparts, are critical tools to investigate and characterize molecular mechanisms underlying gynecologic tumorigenesis, and facilitate development of novel therapeutics. To determine the extent of misidentification, contamination and redundancy, with evident consequences for the validity of research based upon these models, we undertook a systematic analysis and cataloging of endometrial and ovarian cell lines. Methods Profiling of cell lines by analysis of DNA microsatellite short tandem repeats (STR), p53 nucleotide polymorphisms and microsatellite instability. Results Fifty-one ovarian cancer lines were profiled with ten found to be redundant and five (A2008, OV2008, C13, SK-OV-4 and SK-OV-6) identified as cervical cancer cells. Ten endometrial cell lines were analyzed, with RL-92, HEC-1A, HEC-1B, HEC-50, KLE, and AN3CA all exhibiting unique, uncontaminated STR profiles. Multiple variants of Ishikawa and ECC-1 endometrial cancer cell lines were genotyped and analyzed by sequencing of mutations in the p53 gene. The profile of ECC-1 cells did not match the EnCa-101 tumor, from which it was reportedly derived, and all ECC-1 isolates genotyped as Ishikawa cells, MCF-7 breast cancer cells, or a combination thereof. Two normal, immortalized endometrial epithelial cell lines, HES cells and the hTERT-EEC line, were identified as HeLa cervical carcinoma and MCF-7 breast cancer cells, respectively. Conclusions Results demonstrate significant misidentification, duplication, and loss of integrity of endometrial and ovarian cancer cell lines. Authentication by STR DNA profiling is a simple and economical method to verify and validate studies undertaken with these models.
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Cites
Schweppe, R. E., Klopper, J. P., Korch, C., Pugazhenthi, U., Benezra, M., Knauf, J. A., Fagin, J. A., Marlow, L. A., Copland, J. A., Smallridge, R. C., et al. (2008) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2008-1102 · Cited by 563
Self-citationHeLa cells 50 years on: the good, the bad and the ugly
Masters, J. R. (2002) · Nature Reviews Cancer · doi:10.1038/nrc775 · Cited by 560
Check your cultures! A list of cross‐contaminated or misidentified cell lines
Capes‐Davis, A., Theodosopoulos, G., Atkin, I., Drexler, H. G., Kohara, A., MacLeod, R. A. F., Masters, J. R., Nakamura, Y., Reid, Y. A., Reddel, R. R., et al. (2010) · International Journal of Cancer · doi:10.1002/ijc.25242 · Cited by 520
Rae, J. M., Creighton, C. J., Meck, J. M., Haddad, B. R., & Johnson, M. D. (2007) · Breast Cancer Research and Treatment · doi:10.1007/s10549-006-9392-8 · Cited by 374
Cross-contamination of cells in culture
Nelson-Rees, W. A., Daniels, D. W., & Flandermeyer, R. R. (1981) · Science · doi:10.1126/science.6451928 · Cited by 329
Cell line misidentification: the beginning of the end
American Type Culture Collection Standards Development Organization Workgroup ASN-0002 (2010) · Nature Reviews Cancer · doi:10.1038/nrc2852 · Cited by 266
Henrietta lacks, HeLa cells, and cell culture contamination
Lucey, B. P., Nelson-Rees, W. A., & Hutchins, G. M. (2009) · Archives of Pathology & Laboratory Medicine · doi:10.5858/133.9.1463 · Cited by 255
MDA-MB-435 and M14 Cell Lines: Identical but not M14 Melanoma?
Chambers, A. F. (2009) · Cancer Research · doi:10.1158/0008-5472.can-09-1528 · Cited by 218
DNA fingerprinting of the NCI-60 cell line panel
Lorenzi, P. L., Reinhold, W. C., Varma, S., Hutchinson, A. A., Pommier, Y., Chanock, S. J., & Weinstein, J. N. (2009) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-08-0921 · Cited by 166
Persistent use of “false” cell lines
Lacroix, M. (2008) · International Journal of Cancer · doi:10.1002/ijc.23233 · Cited by 151
Cited by
Zondervan, K. T., Becker, C. M., Koga, K., Missmer, S. A., Taylor, R. N., & Viganò, P. (2018) · Nature Reviews Disease Primers · doi:10.1038/s41572-018-0008-5 · Cited by 1,257
Ovarian Cancer Cell Line Panel (OCCP): Clinical Importance of In Vitro Morphological Subtypes
Beaufort, C. M., Helmijr, J. C. A., Piskorz, A. M., Hoogstraat, M., Ruigrok-Ritstier, K., Besselink, N., Murtaza, M., van IJcken, W. F. J., Heine, A. A. J., Smid, M., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0103988 · Cited by 403
“to have a variable STR profile with multiple peaks which may indicate cross-contamination [29].”
BackgroundEnhancer selectivity in space and time: from enhancer–promoter interactions to promoter activation
Yang, J. H., & Hansen, A. S. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-024-00710-6 · Cited by 191
Match criteria for human cell line authentication: Where do we draw the line?
Capes‐Davis, A., Reid, Y. A., Kline, M. C., Storts, D. R., Strauss, E., Dirks, W. G., Drexler, H. G., MacLeod, R. A. F., Sykes, G., Kohara, A., et al. (2013) · International Journal of Cancer · doi:10.1002/ijc.27931 · Cited by 180
“15– 19 Other groups such as the National Institute of Standards and Technology (NIST) are generating STR profiles specifically for the NCBI database through the Identification of Human Cell Lines Project.”
MethodSarfstein, R., Friedman, Y., Attias-Geva, Z., Fishman, A., Bruchim, I., & Werner, H. (2013) · PLoS ONE · doi:10.1371/journal.pone.0061537 · Cited by 150
Metadata
- Authors
- Korch, C., Spillman, M. A., Jackson, T. A., Jacobsen, B. M., Murphy, S. K., Lessey, B. A., Jordan, V. C., & Bradford, A. P.
- Venue
- Gynecologic Oncology
- Publisher
- Elsevier BV
- Published
- 2012-10
- Volume
- 127
- Issue
- 1
- Pages
- 241-248
- ISSN
- 0090-8258 (ISSN-L)
- OpenAlex
- W2163316512
- Cited by
- 251
- References
- 15
- FWCI
- 25.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Korch, C., Spillman, M. A., Jackson, T. A., Jacobsen, B. M., Murphy, S. K., Lessey, B. A., Jordan, V. C., & Bradford, A. P. (2012). DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination. Gynecologic Oncology. https://doi.org/10.1016/j.ygyno.2012.06.017
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