Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae
Abstract
Xylose is the main pentose and second most abundant sugar in lignocellulosic feedstocks. To improve xylose utilization, necessary for the cost-effective bioconversion of lignocellulose, several metabolic engineering approaches have been employed in the yeast Saccharomyces cerevisiae. In this study, we describe the rational metabolic engineering of a S. cerevisiae strain, including overexpression of the Piromyces xylose isomerase gene (XYLA), Pichia stipitis xylulose kinase (XYL3) and genes of the non-oxidative pentose phosphate pathway (PPP). This engineered strain (H131-A3) was used to initialize a three-stage process of evolutionary engineering, through first aerobic and anaerobic sequential batch cultivation followed by growth in a xylose-limited chemostat. The evolved strain H131-A3-AL(CS) displayed significantly increased anaerobic growth rate (0.203±0.006 h⁻¹) and xylose consumption rate (1.866 g g⁻¹ h⁻¹) along with high ethanol conversion yield (0.41 g/g). These figures exceed by a significant margin any other performance metrics on xylose utilization and ethanol production by S. cerevisiae reported to-date. Further inverse metabolic engineering based on functional complementation suggested that efficient xylose assimilation is attributed, in part, to the elevated expression level of xylose isomerase, which was accomplished through the multiple-copy integration of XYLA in the chromosome of the evolved strain.
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A S. cerevisiae strain with deficient pyruvate decarboxylase activity was evolved for high-glucose tolerance.
“In another study, van Maris and co-workers evolved a S. cerevisiae strain with deficient pyruvate decarboxylase activity for high-glucose tolerance [97].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [97]
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Self-citation
- Authors
- Zhou, H., Cheng, J.-S., Wang, B. L., Fink, G. R., & Stephanopoulos, G.
- Affiliations
- Massachusetts Institute of Technology (US)
- Whitehead Institute for Biomedical Research (US)
- Corresponding author
- Stephanopoulos, Gregory
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 14
- Issue
- 6
- Pages
- 611-622
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W2060718895
- Cited by
- 287
- References
- 54
- Citations by year
- 2026: 9 · 2025: 5 · 2024: 12 · 2023: 10 · 2022: 21 · 2021: 14 · 2020: 19 · 2019: 24 · 2018: 28 · 2017: 31 · 2016: 24 · 2015: 29 · 2014: 29 · 2013: 28 · 2012: 4
- FWCI
- 11.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metabolic Engineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- xylose isomerase, Saccharomyces cerevisiae, xylose utilization, ethanol production, pentose phosphate pathway, xylulose kinase, Pichia stipitis, evolutionary engineering, lignocellulosic biomass, xylose uptake, inverse metabolic engineering, functional complementation
- MeSH terms
- Ethanol, Pentose Phosphate Pathway, Pichia, Saccharomyces cerevisiae, Xylose, Protein Engineering, Up-Regulation, Phosphotransferases (Alcohol Group Acceptor), Directed Molecular Evolution, Aldose-Ketose Isomerases, Piromyces, Genetic Enhancement
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