Cited by 241Biofuel production and bioconversionMicrobial Metabolic Engineering and BioproductionFungal and yeast genetics research

Yeast metabolic engineering for hemicellulosic ethanol production

Van Vleet, JH, Jeffries, TW·2009·Current Opinion in Biotechnology

Abstract

Efficient fermentation of hemicellulosic sugars is critical for the bioconversion of lignocellulosics to ethanol. Efficient sugar uptake through the heterologous expression of yeast and fungal xylose/glucose transporters can improve fermentation if other metabolic steps are not rate limiting. Rectification of cofactor imbalances through heterologous expression of fungal xylose isomerase or modification of cofactor requirements in the yeast oxidoreductase pathway can reduce xylitol production while increasing ethanol yields, but these changes often occur at the expense of xylose utilization rates. Genetic engineering and evolutionary adaptation to increase glycolytic flux coupled with transcriptomic and proteomic studies have identified targets for further modification, as have genomic and metabolic engineering studies in native xylose fermenting yeasts.

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Crossref · 10.1016/j.copbio.2009.06.001

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  • Brennan et al. devised an extraction method for glucose and xylose recovery from [C2C1im][MeCO2] using boronates such as naphthalene-2-boronate.

    “Brennan et al. have devised an extraction method for glucose and xylose recovery from [C 2 C 1 im][MeCO 2 ] using boronates such as naphthalene-2-boronate.”

    From Deconstruction of lignocellulosic biomass with ionic liquids (2013) · cites this paper as [182]

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Authors
Van Vleet, J., & Jeffries, T.
Venue
Current Opinion in Biotechnology
Publisher
Elsevier BV
Published
2009-06
Volume
20
Issue
3
Pages
300-306
ISSN
0958-1669 (ISSN-L)
OpenAlex
W2068003115
Cited by
241
References
51
Citations by year
2026: 1 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 15 · 2019: 6 · 2018: 15 · 2017: 15 · 2016: 21 · 2015: 9 · 2014: 26 · 2013: 25 · 2012: 34
FWCI
2.52
Type
journal article
Language
en
Open access
closed
Locations
  • Current Opinion in Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Energy
  • U.S. Department of Agriculture
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
yeast metabolic engineering, xylose fermentation, xylose transporters, xylose isomerase, xylitol production, lignocellulosic biomass, glycolytic flux, xylose-fermenting yeast, heterologous expression
MeSH terms
Ethanol, Aldehyde Reductase, Pichia, Polysaccharides, Xylose, Yeasts, Protein Engineering, Aldose-Ketose Isomerases, D-Xylulose Reductase

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