Cited by 321Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

Open access

Xue, Jiao, Niu, Ying-Fang, Huang, Tan, Yang, Wei-Dong, Liu, Jie-Sheng, Li, Hong-Ye·2015·Metabolic Engineering

DOI 10.1016/j.ymben.2014.10.002

Open this paper in CoChat — free account Open PDF

Abstract

To obtain fast growing oil-rich microalgal strains has been urgently demanded for microalgal biofuel. Malic enzyme (ME), which is involved in pyruvate metabolism and carbon fixation, was first characterized in microalgae here. Overexpression of Phaeodactylum tricornutum ME (PtME) significantly enhanced the expression of PtME and its enzymatic activity in transgenic P. tricornutum. The total lipid content in transgenic cells markedly increased by 2.5-fold and reached a record 57.8% of dry cell weight with a similar growth rate to wild type, thus keeping a high biomass. The neutral lipid content was further increased by 31% under nitrogen-deprivation treatment, still 66% higher than that of wild type. Transgenic microalgae cells exhibited obvious morphological changes, as the cells were shorter and thicker and contained larger oil bodies. Immuno-electron microscopy targeted PtME to the mitochondrion. This study markedly increased the oil content in microalgae, suggesting a new route for developing ideal microalgal strains for industrial biodiesel production.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1016/j.ymben.2014.10.002

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
Tamura, K., Dudley, J. T., Nei, M., & Kumar, S. (2007) · Molecular Biology and Evolution · doi:10.1093/molbev/msm092 · Cited by 28,734
Biodiesel from microalgae
Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Subcutaneous and visceral adipose tissue: Structural and functional differences
Ibrahim, M. M. (2010) · Obesity Reviews · doi:10.1111/j.1467-789x.2009.00623.x · Cited by 2,238
The Genome of the Diatom Thalassiosira Pseudonana : Ecology, Evolution, and Metabolism
Armbrust, E. V., Berges, J. A., Bowler, C., Green, B. R., Martínez, D., Putnam, N. H., Zhou, S., Allen, A. E., Apt, K. E., Bechner, M., et al. (2004) · Science · doi:10.1126/science.1101156 · Cited by 2,029
Improved recovery of fatty acid through direct transesterification without prior extraction or purification.
Lepage, G., & ROY, C. C. (1984) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34457-6 · Cited by 1,028
Challenges in Engineering Microbes for Biofuels Production
Stephanopoulos, G. N. (2007) · Science · doi:10.1126/science.1139612 · Cited by 731
A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae
Chen, W., Zhang, C., Song, L., Sommerfeld, M., & Hu, Q. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.01.001 · Cited by 681
Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence
Jiang, P., Du, W., Mancuso, A., Wellen, K. E., & Yang, X. (2013) · Nature · doi:10.1038/nature11776 · Cited by 491
Characteristics of C4 photosynthesis in stems and petioles of C3 flowering plants
Hibberd, J. M., & Quick, W. P. (2002) · Nature · doi:10.1038/415451a · Cited by 420

Show all 51 references

Sorted by citations

Items 201–300 of 321

The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives
Wang, S., & Hu, Z. (2025) · Plant Communications · doi:10.1016/j.xplc.2025.101519 · Cited by 11
Two marine natural products, penicillide and verrucarin J, are identified from a chemical genetic screen for neutral lipid accumulation effectors in Phaeodactylum tricornutum
Yu, M., Chen, X. X., Jiang, M., & Li, X. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10411-7 · Cited by 10
Enhancing lipid productivity in Nannochloropsis salina by overexpression of endogenous glycerol-3-phosphate dehydrogenase
Koh, H. G., Chang, Y. K., & Kang, N. K. (2023) · Journal of Applied Phycology · doi:10.1007/s10811-023-03141-6 · Cited by 10
Algae as Crop Plants Being a Source of Bioactive Ingredients of Pharmaceutical and Dietary Importance
Jabłońska‐Trypuć, A. (2024) · Agronomy · doi:10.3390/agronomy14050895 · Cited by 11
Combined effects of nitrogen limitation and overexpression of malic enzyme gene on lipid accumulation in Dunaliella parva
Liang, S., Li, L., Ling, M., Mo, L., Huang, L., Ruan, L., & Shang, C. (2024) · Bioresource Technology · doi:10.1016/j.biortech.2024.131291 · Cited by 10
Green strategies for enhanced microalgae processes: Leveraging bio-derived adsorbents, green solvents, and synthetic biology
Abuhasheesh, Y. H., Ghazal, A., Tang, D. Y. Y., BANAT, F. A., Hasan, S. W., & Show, P. L. (2025) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2025.115986 · Cited by 10
The boosted lipid accumulation in microalga Chlorella vulgaris by a heterotrophy and nutrition-limitation transition cultivation regime
Liu, T., Liu, F., Wang, C., Wang, Z., & Li, Y. (2016) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-016-2164-7 · Cited by 10
A cheap two-step cultivation of Phaeodactylum tricornutum for increased TAG production and differential expression of TAG biosynthesis associated genes
Karpagam, R., Jawaharraj, K., Ashokkumar, B., Pugazhendhi, A., & Varalakshmi, P. (2022) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2022.06.002 · Cited by 10
The possibility of using marine diatom-infecting viral promoters for the engineering of marine diatoms
Kadono, T., Tomaru, Y., Suzuki, K., Yamada, K., & Adachi, M. (2020) · Plant Science · doi:10.1016/j.plantsci.2020.110475 · Cited by 10
Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae
Chintagunta, A. D., Kumar, S. P. J., & KUMAR, N. S. S. (2024) · Biocell · doi:10.32604/biocell.2024.050540 · Cited by 10
Peroxisomal biogenesis factor 11 as a novel target to trigger lipid biosynthesis and salt stress resistance in oleaginous Tetradesmus obliquus
Xue, Y., Wang, W., Sheng, X., Zheng, Z., Wang, Z., Ding, F., Li, J., Sun, Z., Cai, Y., Wang, X., et al. (2025) · Bioresource Technology · doi:10.1016/j.biortech.2025.132209 · Cited by 9
Self-citation
Biofuel production
Nunes, N. S. P., Ansilago, M., de Oliveira, N. N., Leite, R. S. R., da Paz, M. F., & Fonseca, G. G. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-821218-9.00006-2 · Cited by 11
Advanced Gene Technology and Synthetic Biology Approaches to Custom Design Microalgae for Biodiesel Production
Arora, N., Tripathi, S., Poluri, K. M., & Pruthi, V. (2019) · Springer eBooks · doi:10.1007/978-981-13-2264-8_8 · Cited by 9
Genome editing of algal species by CRISPR Cas9 for biofuels
Tanwar, A., & Kumar, S. B. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818140-9.00015-5 · Cited by 9
Diatom triacylglycerol metabolism: from carbon fixation to lipid droplet degradation
Murison, V., Hérault, J., Marchand, J., & Ulmann, L. (2025) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.70006 · Cited by 8
Physiochemical and gene expression analyses reveal differential responses of the marine oleaginous alga Nannochloropsis salina under different lipid-induction conditions
Ma, X., Liu, B., Yang, B., Guo, B., Liu, J., & Chen, F. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-017-1318-9 · Cited by 9
Self-citation
Enhancement of Lipid Productivity and Self-flocculation by Cocultivating Monoraphidium sp. FXY-10 and Heveochlorella sp. Yu Under Mixotrophic Mode
Feng, Y., Xiao, J., Cui, N., Zhao, Y., & Zhao, P. (2021) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-021-03593-x · Cited by 9
Metallurgical slag used for efficient growth of Chlorella pyrenoidosa to achieve CO2 conversion to biodiesel
Guo, H., Wang, Y., Liu, H., Zeng, Y., Wang, W., Liu, T., Kang, L., Ji, R., Wang, Y., Li, J., et al. (2024) · Carbon Neutralization · doi:10.1002/cnl2.122 · Cited by 9
Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11
Fazili, A. B. A., Shah, A. M., Albeshr, M. F., Naz, T., Dar, M. A., Yang, W., Garre, V., Fazili, K. M., Bhat, E. A., & Song, Y. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.919364 · Cited by 8
Self-citation
RNA-seq analysis reveals genes related to photosynthetic carbon partitioning and lipid production in Phaeodactylum tricornutum under alkaline conditions
Liu, J., Yin, W., Zhang, X., Xie, X., Dong, G., Lü, Y., Tao, B., Gong, Q., Chen, X., Shi, C., et al. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.969639 · Cited by 9
Self-citation
Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling
Liang, S., Guo, L., Lin, G., Zhang, Z., Ding, H., Wang, Y., & Yang, G. (2016) · Chinese Journal of Oceanology and Limnology · doi:10.1007/s00343-017-6023-7 · Cited by 8
Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential
Carrier, G., Berthelier, J., Maupetit, A., Nicolau, É., Marbouty, M., Schreiber, N., Charrier, A., Carcopino, C., Leroi, L., & Saint‐Jean, B. (2023) · European Journal of Phycology · doi:10.1080/09670262.2023.2249073 · Cited by 7
Biodiesel Production from Oleaginous Microorganisms with Organic Wastes as Raw Materials
Chen, J., Zhang, X., Yan, S., Tyagi, R. D., Surampalli, R. Y., & Zhang, T. C. (2019) · American Society of Civil Engineers eBooks · doi:10.1061/9780784415344.ch10 · Cited by 7
Use of tar color additives as a light filter to enhance growth and lipid production by the microalga Nannochloropsis gaditana
Shin, W.-S., Jung, S. M., Cho, C., Woo, D.-W., Kim, W., & Kwon, J.-H. (2018) · Environmental Engineering Research · doi:10.4491/eer.2017.162 · Cited by 7
Potential of Microalgae Biomass for the Sustainable Production of Bio-commodities
Perin, G., & Morosinotto, T. (2019) · Progress in botany · doi:10.1007/124_2019_30 · Cited by 7
Towards the Genetic Manipulation of Microalgae to Improve the Carbon Dioxide Fixation and the Production of Biofuels: Present Status and Future Prospect
Díaz‐Santos, E. (2019) · Springer eBooks · doi:10.1007/978-981-13-2264-8_7 · Cited by 8
Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella SalinaPreprint
Dai, J.-L., He, Y., Chen, H., & Jiang, J. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4474009 · Cited by 7
Transcriptional control in microalgae: co-regulated fatty acid biosynthesis and carbon dioxide fixation
Kayani, S., Hu, X., Shen, Q., Zou, B., Zhu, F., Yu, Z., Shah, M. A. R., Rehman, O. U., & Huo, S. (2025) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2025.2503788 · Cited by 6
Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain Mucor circinelloides WJ11
Fazili, A. B. A., Shah, A. M., Naz, T., Nosheen, S., Yang, W., Garre, V., Majeed, Y., Al-Sadoon, M. K., & Song, Y. (2022) · Journal of Fungi · doi:10.3390/jof8030265 · Cited by 7
Self-citation
Engineering Microalgae: Transition from Empirical Design to Programmable Cells
Lu, Y., Zhang, X., Lin, H., & Melis, A. (2023) · CABI eBooks · doi:10.1079/9781800621954.0001 · Cited by 6
Less variability when growing faster? Experimental assessment of the relationship of growth rate with functional traits of the marine diatom Phaeodactylum tricornutum
Klip, H. C. L., Meunier, C. L., & Boersma, M. (2024) · Hydrobiologia · doi:10.1007/s10750-023-05444-8 · Cited by 6
Heterologous glucose-6-phosphate dehydrogenase empowers the biofuel potential of Tetradesmus obliquus via generating lipogenic NADPH
Xue, J., Wang, W., Xue, Y., Li, B., Shi, Y., Li, T., Li, D., Wang, X., Balamurugan, S., & Li, H. (2024) · Algal Research · doi:10.1016/j.algal.2024.103607 · Cited by 6
Self-citation
Transformation of coccolithophorid Emiliania huxleyi harboring a marine virus (Coccolithoviruses) serine palmitoyltransferase (SPT) gene by electroporation
Cai, W., Wang, X., Su, J., Li, J., Zeng, J., Li, G., & Liu, J. (2020) · Journal of Oceanology and Limnology · doi:10.1007/s00343-020-9325-0 · Cited by 6
Self-citation
Advances in Genetic Engineering of Microalgae
Hallmann, A. (2019) · Grand challenges in biology and biotechnology · doi:10.1007/978-3-030-25233-5_5 · Cited by 6
Synthetic biology applied to microalgae-based processes and products
Saini, D. K., Pabbi, S., Prakash, A., & Shukla, P. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818536-0.00004-x · Cited by 6
Constraint-Based Modeling of Diatoms Metabolism and Quantitative Biology Approaches
Kumar, M., Zúñiga, C., Tibocha‐Bonilla, J. D., Smith, S. R., Coker, J. K. C., Allen, A. E., & Zengler, K. (2022) · Springer eBooks · doi:10.1007/978-3-030-92499-7_26 · Cited by 5
Synthetic Biology-Based Advanced Biotechnological Approach in Microalgal Biorefinery
Zafar, S. U., Mehra, A., & Jutur, P. P. (2022) · Clean Energy Production Technologies · doi:10.1007/978-981-19-0680-0_10 · Cited by 7
Silencing 1,3-β-glucan synthase gene promotes total lipid production and changes fatty acids composition by affecting carbon flow distribution in Phaeodactylum tricornutum
JIANG, X. H., Zhu, B., Tu, C., Li, Y., Zhao, Y., Yang, G., & Pan, K. (2022) · Algal Research · doi:10.1016/j.algal.2022.102827 · Cited by 6
Phenotype stability and dynamics of transposable elements in a strain of the microalga Tisochrysis lutea with improved lipid traits
Berthelier, J., Saint‐Jean, B., Casse, N., Bougaran, G., & Carrier, G. (2023) · PLoS ONE · doi:10.1371/journal.pone.0284656 · Cited by 6
Autotrophic Cultivation of Microalgae for the Production of Polyunsaturated Fatty Acid
Saxena, P., Kumar, M., & Harish (2020) · Wiley eBooks · doi:10.1002/9781119631729.ch6 · Cited by 6
Sustainable bioenergy from microalgal lipid remodeling: An AI and genetic engineering approach for the circular economy
Singh, R. P., Singh, P. K., Kumar, I., Kumar, M., Gaur, V. K., Kaushik, A., Arya, A., Saikia, M., Santos‐Villalobos, S. D. L., Kumar, A., et al. (2026) · Energy Strategy Reviews · doi:10.1016/j.esr.2026.102050 · Cited by 5
Paclobutrazol induces the concurrent accumulation of chrysolaminarin and lipids in the diatom Phaeodactylum tricornutum
Yang, Y., Lü, Y., Zheng, J.-W., Li, D., Wang, X., Yang, W., & Li, H. (2023) · Journal of Oceanology and Limnology · doi:10.1007/s00343-022-2179-x · Cited by 5
Self-citation
Genetic Engineering Tools for Enhancing Lipid Production in Microalgae
Kumari, S., Singh, P., Gupta, S. K., & Kumar, S. (2015) · Springer eBooks · doi:10.1007/978-81-322-2641-3_10 · Cited by 5
MICROALGAL BIOTECHNOLOGY: ETHICS AND INTELLECTUAL PROPERTY RIGHTS
Köse, A., & Öncel, S. Ş. (2016) · Deu Muhendislik Fakultesi Fen ve Muhendislik · doi:10.21205/deufmd.20165217552 · Cited by 5
Expression Profile of Genes Involved in Isoprenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum
Kira, N., Yoshimatsu, T., Fukunaga, K., Okada, S., Adachi, M., & Kadono, T. (2016) · Environment Control in Biology · doi:10.2525/ecb.54.31 · Cited by 5
Molecular Engineering/Metabolic Engineering-Based Advanced Biotechnological Approach in Microalgal Biorefinery
Vidya, D., & Arunkumar, K. (2022) · Clean Energy Production Technologies · doi:10.1007/978-981-19-0680-0_7 · Cited by 5
Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina
Dai, J.-L., He, Y., Chen, H., & Jiang, J. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c04810 · Cited by 11
Evaluation of secretory signal peptides for heterologous protein secretion in Cyanobacterium aponinum PCC10605
Nandru, R., Bhadra, B., Roy, N., Nigam, A., & Suprasanna, P. (2025) · Biotechnology Letters · doi:10.1007/s10529-025-03569-5 · Cited by 4
Regulatory network rewiring drives strain‐specific lipid accumulation response in Chlorella sorokiniana under nutrient starvation
Barrera‐Duarte, C. C., Montes, R. A. C., Nájera‐González, H., & López-Arredondo, D. L. (2025) · The Plant Journal · doi:10.1111/tpj.70644 · Cited by 4
Effects of Mn2+ on neutral lipid content, C4 pathway, and related gene expression in Phaeodactylum tricornutum
Chen, R.-S., Zhang, L., Wei, F.-J., Yuan, L.-Y., Zhao, P., Wang, H., & Gong, Y. (2021) · Journal of Applied Phycology · doi:10.1007/s10811-021-02471-7 · Cited by 4
Genetic manipulation of microalgae for enhanced biotechnological applications
Pandey, A., Kant, G., Afzal, S., Singh, M. P., Singh, N. K., Kumar, S., & Srivastava, S. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-823764-9.00019-4 · Cited by 4
The refinery concept: addressing the challenges of microalgal biodiesel production
Patnaik, R., Bagchi, S. K., & Mallick, N. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819597-0.00010-6 · Cited by 4
Regulatory role of death specific protein in response to nutrient limitation in a marine diatom
Hao, T.-B., Balamurugan, S., Yang, W., Wang, X., & Li, H. (2021) · Algal Research · doi:10.1016/j.algal.2021.102392 · Cited by 4
Self-citation
Cutting-edge approaches for overcoming challenges in microalgal biodiesel production
Mondal, A. S., Sharma, R., & Trivedi, N. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-13927-7.00022-0 · Cited by 4
Impact of heterologous expression of Cannabis sativa tetraketide synthase on Phaeodactylum tricornutum metabolic profile
Sene, N., dos Santos, K. C. G., Mérindol, N., Gélinas, S.-E., Custeau, A., Awwad, F., Fantino, E., Meddeb-Mouelhi, F., Germain, H., & Desgagné-Penix, I. (2025) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-025-02638-1 · Cited by 3
Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom
Chen, Y., Geng, L., Hao, Z., Ding, N., Di, J., Hou, H., Zhang, L., & Wang, H. (2025) · Microbial Cell Factories · doi:10.1186/s12934-025-02893-9 · Cited by 3
Revolutionizing Brain Drug Delivery: Buccal Transferosomes on the Verge of a Breakthrough
Chandrasekhar, P., & Kaliyaperumal, R. (2024) · Recent Advances in Drug Delivery and Formulation · doi:10.2174/0126673878312336240802113811 · Cited by 3
Algal lipids, lipidomics, and biosurfactants
Reddy, M. M. S., Tiwari, S., & Chauhan, V. S. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-18816-9.00019-8 · Cited by 3
Three-Dimensional Visualisation and Quantification of Lipids in Microalgae Using Confocal Laser Scanning Microscopy
Chansawang, N., Obara, B., Geider, R. J., & Laissue, P. P. (2015) · Springer protocols handbooks/Springer protocols · doi:10.1007/8623_2015_142 · Cited by 4
Chemicals and Fuels from Microalgae
Jeon, S., Jeong, B., & Chang, Y. K. (2017) · Springer eBooks · doi:10.1007/978-3-319-50436-0_384 · Cited by 3
Utilization of Microalgae and Thraustochytrids for the Production of Biofuel and Nutraceutical Products
Liu, Y., & Cheng, J. J. (2021) · Wiley eBooks · doi:10.1002/9783527825394.ch7 · Cited by 3
Boosting Lipid Production in the Diatom Phaeodactylum tricornutum by Knockdown of the Enoyl CoA Hydratase Using CRISPR InterferencePreprint
Guo, W., Gao, Y., Weng, Y., Chang, C., Huang, X., & Zhang, F. (2023) · Research Square · doi:10.21203/rs.3.rs-2937793/v1 · Cited by 3
Exogenous melatonin alleviates ultrasound stress in Dunaliella salina via physiological responses
Kan, X., Liu, Y., & Liu, C. (2026) · Process Biochemistry · doi:10.1016/j.procbio.2026.04.002 · Cited by 2
Microalgal farming for biofuel production: Extraction, conversion, and characterization
Mondal, S., Halder, S. K., & Mondal, K. C. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-13927-7.00006-2 · Cited by 2
Strategies for increased production of lipids and fine chemicals from commercially important microalgae
Khadim, S. R., Mohanta, A., Maurya, P., Singh, P., & Asthana, R. K. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-820655-3.00008-2 · Cited by 3
Biodiesel from oleaginous fungi, bacteria, and yeast produced using waste substrates
Singh, H. M., Goria, K., Raina, S., Azam, R., Kothari, R., Arora, N. K., & Tyagi, V. V. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-823958-2.00004-5 · Cited by 2
Biodiesel from Microalgae
Nicolò, M. S., Guglielmino, S. P. P., Solinas, V., & Salis, A. (2016) · Springer eBooks · doi:10.1007/978-3-319-31421-1_210-1 · Cited by 2
Enhancing Acetate Utilization in Phaeodactylum tricornutum through the Introduction of Acetate Transport Protein
Song, P., Ma, N., Dong, S., Qiao, H., Zhang, J., Guan, B., Tong, S., & Zhao, Y. (2024) · Biomolecules · doi:10.3390/biom14070822 · Cited by 1
Diatoms as producers of fucoxanthin and polyunsaturated fatty acids
Avsiyan, A. L., & Гудвилович, И. Н. (2025) · Učënye zapiski Kazanskogo universiteta. Seriâ Estestvennye nauki/Učënye zapiski Kazanskogo universiteta. Seriâ Estestvennye nauki/Učenye zapiski Kazanskogo gosudarstvennogo universiteta. Seriâ Estestvennye nauki · doi:10.26907/2542-064x.2025.3.399-440 · Cited by 1
Using the hydrolysate of Chinese medicine residues as nutrient source improves the performance of oil-producing algae: Phenomena and mechanistic elucidation
Chen, S., Guo, R., Zheng, Z., Chen, W., Ma, C., Liu, Y., Chen, J., & Ke, J. (2025) · Bioresource Technology · doi:10.1016/j.biortech.2025.133737 · Cited by 1
Potential of CRISPR/Cas9 Genome Engineering for Biofuel Production From Economically Relevant Algae
Kumari, S., Phillips, A. J., Adelodun, B., Maria, F. D., Sundaramurthy, S. K., & Pal, T. (2026) · International Journal of Energy Research · doi:10.1155/er/2368855 · Cited by 1
Comparative Genomic and Transcriptomic Analyses of Microalgae as Biofactories
Menin, B., Branduardi, P., & Mariani, P. (2015) · Chemical engineering transactions · doi:10.3303/cet1543200 · Cited by 1
Biodiesel from Microalgae
Nicolò, M. S., Guglielmino, S. P. P., Solinas, V., & Salis, A. (2017) · Springer eBooks · doi:10.1007/978-3-319-50436-0_210 · Cited by 1
Microalgae for Energy
Razeghifard, R. (2016) · Wiley eBooks · doi:10.1002/0471238961.koe00019 · Cited by 1
Photosynthetic efficiency in microalgal lipid production
Remmers, I. M. (2017) · doi:10.18174/416933 · Cited by 1
Current Advances in Biotechnology of Marine Microalgae
Wan, C., Chen, B., Zhao, X., & Bai, F. (2020) · Wiley eBooks · doi:10.1002/9781119143802.ch77 · Cited by 1
MICROALGAL BIOTECHNOLOGY: ETHICS AND INTELLECTUAL PROPERTY RIGHTS (MİKROALGAL BİYOTEKNOLOJİ: ETİK VE FİKRİ MÜKLİYET HAKLARI)
Öncel, S. Ş. (2016)
Transcriptional Engineering for Enhancing Valuable Components in Photosynthetic Microalgae
Balamurugan, S., Li, D., Wang, X., Yang, W., Liu, J., & Li, H. (2019) · Springer eBooks · doi:10.1007/978-981-13-9624-3_16
Self-citation
Diversity and engineering of diatom metabolism for new and improved sterol products
Madrid, J., & Cristina, A. (2020) · UTS ePRESS (University of Technology Sydney)
Chemicals and Fuels from Microalgae
Jeon, S., Jeong, B., & Chang, Y. K. (2017) · Springer eBooks · doi:10.1007/978-3-319-31421-1_384-2 · Cited by 1
Metabolic Engineering and Genome Editing Strategies for Enhanced Lipid Production in Oleaginous MicroorganismsPreprint
Chintagunta, A. D., Kumar, N. S. S., Kumar, P. N., Kumar, P. S., & Kumar, S. P. J. (2022) · SSRN Electronic Journal · doi:10.2139/ssrn.4104119
Genetically Modified (GM) Microalgae for Biofuel Production
Bajpai, P. (2022) · SpringerBriefs in applied sciences and technology · doi:10.1007/978-981-19-2001-1_2
The Use of Omics Technologies, Random Mutagenesis, and Genetic Transformation Techniques to Improve Algae for Biodiesel Industry
Adıgüzel, A. O. (2022) · Clean Energy Production Technologies · doi:10.1007/978-981-19-6806-8_2
Genetic engineering of algae material
Shailaja, V. L., John, C. M., & Kalaivani, M. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-18816-9.00012-5
Microalgae as biofuel: current perspectives and technological progress
Gupta, S., & Ahirwar, S. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-95332-0.00011-9
The Effect of Light and Salinity on the Abundance and Biomass of Skeletonema Sp. Microalgae
(2023) · International Journal of Research Publication and Reviews · doi:10.55248/gengpi.4.523.40242
Whole Genomic Sequencing and Bioinformatic Analysis of Arthrospira Platensis SBC
Teng, J., & Xie, Z. (2023) · International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB) · doi:10.1145/3586139.3586144
Gene expression and lipid accumulation in green microalga chlamydomonas reinhardtii (137c) under salt stress condition
Atikij, T., & Waditee-Sirisattha, R. (2018) · doi:10.58837/chula.the.2018.369
Caractérisation des glycérolipides et de la dynamique de remodelage en chaines acyles chez Ostreococcus tauri
Degraeve-Guilbault, C. (2017) · theses.fr (ABES) · doi:10.70675/b737f152zc60fz42f2z9875zfd46ea0d165b
Assessment of wastes for future bioprospecting
Kushwaha, J., Rani, J., Priyadarsini, M., Pandey, K. P., & Dhoble, A. S. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-95179-1.00002-5
Control of biological contamination in microalgae cultures
Flores-Tinoco, V., Brito-Bello, A. A., Le, V. P., & López-Arredondo, D. L. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-14058-7.00005-1
Process intensification approaches applied to microalgae bioreactors
Caltzontzin-Rabell, V., Morales, J. M. V., Guzmán-Martínez, C. E., Hernández, S., Martínez-Guido, S. I., & Gutiérrez‐Antonio, C. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-14058-7.00038-5
Algae feedstock generation
Mohanty, K., & Mohanty, S. S. (2025) · Elsevier eBooks · doi:10.1016/b978-0-323-90840-5.00003-4
Exogenous Melatonin Alleviates Ultrasound Stress in Dunaliella salina via Physiological and Molecular ResponsesPreprint
Kan, X., Liu, Y., & Liu, C. (2025) · SSRN Electronic Journal · doi:10.2139/ssrn.5762803
Microalgae-derived biodiesel: Review on microalgae species, genetic modification, cultivation strategies, and mutagenesis
Tian, J., Lin, J., Zhuang, G., Sun, P., Geng, P., Chen, L., & Chen, X. (2025) · Algal Research · doi:10.1016/j.algal.2025.104490
Additional file 5 of Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis
Cui, H., Zhao, C., Xu, W., Zhang, H., Hang, W., Zhu, X., Ji, C., Xue, J., Zhang, C., & Li, R. (2021) · Figshare · doi:10.6084/m9.figshare.13532351
Self-citation
Additional file 7 of Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis
Cui, H., Zhao, C., Xu, W., Zhang, H., Hang, W., Zhu, X., Ji, C., Xue, J., Zhang, C., & Li, R. (2021) · Figshare · doi:10.6084/m9.figshare.13532357
Self-citation
Additional file 10 of Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis
Cui, H., Zhao, C., Xu, W., Zhang, H., Hang, W., Zhu, X., Ji, C., Xue, J., Zhang, C., & Li, R. (2021) · Figshare · doi:10.6084/m9.figshare.13532333
Self-citation
Additional file 6 of Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis
Cui, H., Zhao, C., Xu, W., Zhang, H., Hang, W., Zhu, X., Ji, C., Xue, J., Zhang, C., & Li, R. (2021) · Figshare · doi:10.6084/m9.figshare.13532354
Self-citation
Additional file 1 of Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis
Cui, H., Zhao, C., Xu, W., Zhang, H., Hang, W., Zhu, X., Ji, C., Xue, J., Zhang, C., & Li, R. (2021) · Figshare · doi:10.6084/m9.figshare.13532339
Self-citation

Authors: Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y.
Affiliations: Jinan University (CN)
ORCID: Xue, Jiao
Niu, Ying-Fang
Yang, Wei-Dong
Li, Hong-Ye
Venue: Metabolic Engineering
Publisher: Elsevier BV
Published: 2015-01
Volume: 27
Pages: 1-9
ISSN: 1096-7176 (ISSN-L)
DOI: 10.1016/j.ymben.2014.10.002
OpenAlex: W1986227133
Cited by: 321
References: 51
Citations by year: 2026: 12 · 2025: 20 · 2024: 22 · 2023: 31 · 2022: 30 · 2021: 41 · 2020: 34 · 2019: 36 · 2018: 26 · 2017: 27 · 2016: 29 · 2015: 13
FWCI: 7.59
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-sa
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by-nc-sa/3.0/ (vor)
Locations: Metabolic Engineering (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
Funders: National Science and Technology Support Program (2011BAD14B03)
Science and Technology Project of Guangdong (2009B050600005, 2010B030600005)
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords: Phaeodactylum tricornutum, malic enzyme, neutral lipid accumulation, transgenic microalgae, nitrogen starvation, oil bodies, mitochondrial targeting, pyruvate metabolism, CO2 fixation, microalgae biodiesel, genetic improvement
MeSH terms: Genetic Engineering, Malate Dehydrogenase, Gene Expression, Diatoms, Algal Proteins, Organisms, Genetically Modified, Lipid Metabolism

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks