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Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation
Open accessAbstract
To obtain fast growing oil-rich microalgal strains has been urgently demanded for microalgal biofuel. Malic enzyme (ME), which is involved in pyruvate metabolism and carbon fixation, was first characterized in microalgae here. Overexpression of Phaeodactylum tricornutum ME (PtME) significantly enhanced the expression of PtME and its enzymatic activity in transgenic P. tricornutum. The total lipid content in transgenic cells markedly increased by 2.5-fold and reached a record 57.8% of dry cell weight with a similar growth rate to wild type, thus keeping a high biomass. The neutral lipid content was further increased by 31% under nitrogen-deprivation treatment, still 66% higher than that of wild type. Transgenic microalgae cells exhibited obvious morphological changes, as the cells were shorter and thicker and contained larger oil bodies. Immuno-electron microscopy targeted PtME to the mitochondrion. This study markedly increased the oil content in microalgae, suggesting a new route for developing ideal microalgal strains for industrial biodiesel production.
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“However, P. tricornutum is readily stained by the lipophilic fluorophore Nile Red26, making the use of CARS microscopy for this microalga valuable, but not critical.”
From Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy (2016) · cites this paper as [26]
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Metadata
- Authors
- Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y.
- Affiliations
- Jinan University (CN)
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 27
- Pages
- 1-9
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W1986227133
- Cited by
- 321
- References
- 51
- Citations by year
- 2026: 12 · 2025: 20 · 2024: 22 · 2023: 31 · 2022: 30 · 2021: 41 · 2020: 34 · 2019: 36 · 2018: 26 · 2017: 27 · 2016: 29 · 2015: 13
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Metabolic Engineering (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Science and Technology Support Program (2011BAD14B03)
- Science and Technology Project of Guangdong (2009B050600005, 2010B030600005)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- Phaeodactylum tricornutum, malic enzyme, neutral lipid accumulation, transgenic microalgae, nitrogen starvation, oil bodies, mitochondrial targeting, pyruvate metabolism, CO2 fixation, microalgae biodiesel, genetic improvement
- MeSH terms
- Genetic Engineering, Malate Dehydrogenase, Gene Expression, Diatoms, Algal Proteins, Organisms, Genetically Modified, Lipid Metabolism
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