Cited by 321Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

Open access

Xue, Jiao, Niu, Ying-Fang, Huang, Tan, Yang, Wei-Dong, Liu, Jie-Sheng, Li, Hong-Ye·2015·Metabolic Engineering

DOI 10.1016/j.ymben.2014.10.002

Open this paper in CoChat — free account Open PDF

Abstract

To obtain fast growing oil-rich microalgal strains has been urgently demanded for microalgal biofuel. Malic enzyme (ME), which is involved in pyruvate metabolism and carbon fixation, was first characterized in microalgae here. Overexpression of Phaeodactylum tricornutum ME (PtME) significantly enhanced the expression of PtME and its enzymatic activity in transgenic P. tricornutum. The total lipid content in transgenic cells markedly increased by 2.5-fold and reached a record 57.8% of dry cell weight with a similar growth rate to wild type, thus keeping a high biomass. The neutral lipid content was further increased by 31% under nitrogen-deprivation treatment, still 66% higher than that of wild type. Transgenic microalgae cells exhibited obvious morphological changes, as the cells were shorter and thicker and contained larger oil bodies. Immuno-electron microscopy targeted PtME to the mitochondrion. This study markedly increased the oil content in microalgae, suggesting a new route for developing ideal microalgal strains for industrial biodiesel production.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1016/j.ymben.2014.10.002

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
Tamura, K., Dudley, J. T., Nei, M., & Kumar, S. (2007) · Molecular Biology and Evolution · doi:10.1093/molbev/msm092 · Cited by 28,734
Biodiesel from microalgae
Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Subcutaneous and visceral adipose tissue: Structural and functional differences
Ibrahim, M. M. (2010) · Obesity Reviews · doi:10.1111/j.1467-789x.2009.00623.x · Cited by 2,238
The Genome of the Diatom Thalassiosira Pseudonana : Ecology, Evolution, and Metabolism
Armbrust, E. V., Berges, J. A., Bowler, C., Green, B. R., Martínez, D., Putnam, N. H., Zhou, S., Allen, A. E., Apt, K. E., Bechner, M., et al. (2004) · Science · doi:10.1126/science.1101156 · Cited by 2,029
Improved recovery of fatty acid through direct transesterification without prior extraction or purification.
Lepage, G., & ROY, C. C. (1984) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34457-6 · Cited by 1,028
Challenges in Engineering Microbes for Biofuels Production
Stephanopoulos, G. N. (2007) · Science · doi:10.1126/science.1139612 · Cited by 731
A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae
Chen, W., Zhang, C., Song, L., Sommerfeld, M., & Hu, Q. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.01.001 · Cited by 681
Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence
Jiang, P., Du, W., Mancuso, A., Wellen, K. E., & Yang, X. (2013) · Nature · doi:10.1038/nature11776 · Cited by 491
Characteristics of C4 photosynthesis in stems and petioles of C3 flowering plants
Hibberd, J. M., & Quick, W. P. (2002) · Nature · doi:10.1038/415451a · Cited by 420

Flagged first, then by citations

Show all 51 references

Sorted by citations

Items 101–200 of 321

A Review of Diatom Lipid Droplets
Leyland, B., Boussiba, S., & Khozin‐Goldberg, I. (2020) · Biology · doi:10.3390/biology9020038 · Cited by 57
Enhancement of lipid production in Nannochloropsis salina by overexpression of endogenous NADP-dependent malic enzyme
Jeon, S., Koh, H. G., Cho, J. M., Kang, N. K., & Chang, Y. K. (2021) · Algal Research · doi:10.1016/j.algal.2021.102218 · Cited by 50
Methodological review of genetic engineering approaches for non-model algae
Mosey, M., Douchi, D., Knoshaug, E. P., & Laurens, L. M. L. (2021) · Algal Research · doi:10.1016/j.algal.2021.102221 · Cited by 54
Engineering microalgae: transition from empirical design to programmable cells
Lu, Y., Zhang, X., Gu, X., Lin, H., & Melis, A. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1917507 · Cited by 52
Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy
Jaeger, D., Pilger, C., Hachmeister, H., Oberländer, E., Wördenweber, R., Wichmann, J., Mussgnug, J. H., Huser, T., & Kruse, O. (2016) · Scientific Reports · doi:10.1038/srep35340 · Cited by 47
Fourth generation biofuel from genetically modified algal biomass for bioeconomic development
Shokravi, H., Heidarrezaei, M., Shokravi, Z., Ong, H. C., Lau, W. J., Din, M. F. M., & Ismail, A. F. (2022) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2022.10.010 · Cited by 49
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae
Kuo, E. Y., Yang, R., Chin, Y. Y., Chien, Y., Chen, Y. C., Wei, C., Kao, L., Chang, Y., Li, Y., Chen, T., et al. (2022) · Biotechnology Journal · doi:10.1002/biot.202100603 · Cited by 51
Biodiesel from oleaginous microbes: opportunities and challenges
Gujjala, L. K. S., Kumar, S. P. J., Talukdar, B., Dash, A., Kumar, S., Sherpa, K. C., & Banerjee‬‬‬‬‬‬‬‬‬, R. (2017) · Biofuels · doi:10.1080/17597269.2017.1402587 · Cited by 50
Insights about sustainable biodiesel production from microalgae biomass: A review
Soosai, M. R., Sundaramahalingam, M. A., Shivamthi, C. S., Rajaram, S. K., Varalakshmi, P., Sankar, K., Jeyaram, K., Raja, V. K., Shanmugam, S., Moorthy, I. G., et al. (2020) · International Journal of Energy Research · doi:10.1002/er.6138 · Cited by 48
LED light stress induced biomass and fatty acid production in microalgal biosystem, Acutodesmus obliquus
Choi, Y., Kumaran, R. S., Jeon, H. J., Song, H. J., Yang, Y., Lee, S. H., Song, K. G., Kim, K. J., Singh, V., & Kim, H. J. (2015) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2015.03.035 · Cited by 48
High silicate concentration facilitates fucoxanthin and eicosapentaenoic acid (EPA) production under heterotrophic condition in the marine diatom Nitzschia laevis
Mao, X., Chen, S. H. Y., Lu, X., Yu, J., & Liu, B. (2020) · Algal Research · doi:10.1016/j.algal.2020.102086 · Cited by 51
Genetic Engineering for Enhancement of Biofuel Production in Microalgae
Chaturvedi, V., Goswami, R. K., & Verma, P. (2020) · Clean Energy Production Technologies · doi:10.1007/978-981-15-9593-6_21 · Cited by 46
Identification of a putative patatin-like phospholipase domain-containing protein 3 (PNPLA3) ortholog involved in lipid metabolism in microalga Phaeodactylum tricornutum
Wang, X., Liu, Y., Hu, D., Balamurugan, S., Yang, L., Yang, W., Liu, J., & Li, H. (2015) · Algal Research · doi:10.1016/j.algal.2015.09.005 · Cited by 46
Self-citation
Phytosterol biosynthesis and production by diatoms (Bacillariophyceae)
Jaramillo-Madrid, A. C., Ashworth, J. R., Fabris, M., & Ralph, P. J. (2019) · Phytochemistry · doi:10.1016/j.phytochem.2019.03.018 · Cited by 44
Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum
Erdene-Ochir, E., Shin, B., Kwon, B., Jung, C., & Pan, C. (2019) · Scientific Reports · doi:10.1038/s41598-019-45786-9 · Cited by 44
Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae
Zhang, Y., Pan, Y., Ding, W., Hu, H., & Liu, J. (2020) · GCB Bioenergy · doi:10.1111/gcbb.12771 · Cited by 44
Self-citation
Recent breakthroughs in integrated biomolecular and biotechnological approaches for enhanced lipid and carotenoid production from microalgae
Ahmad, A., BANAT, F. A., AlSafar, H. S., & Hasan, S. W. (2022) · Phytochemistry Reviews · doi:10.1007/s11101-022-09804-5 · Cited by 43
Recent aspects of algal biomass for sustainable fuel production: a review
Jha, S., Singh, R., Pandey, B. K., Tiwari, A. K., Shukla, S. K., Dikshit, A., & Bhardwaj, A. K. (2024) · Discover Sustainability · doi:10.1007/s43621-024-00472-3 · Cited by 46
The generational shift in biofuels: A path toward sustainable energy solutions
Kant, G., Hasan, A., Yadav, P., Pandey, A., & Srivastava, S. (2025) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2025.107757 · Cited by 49
Metabolic Engineering of Microalgae for Biofuel Production
Naghshbandi, M. P., Tabatabaei, M., Aghbashlo, M., Aftab, M. N., & Iqbal, I. (2019) · Methods in molecular biology · doi:10.1007/7651_2018_205 · Cited by 43
Microalgae: the next best alternative to fossil fuels after biomass. A review
Bhagea, R., Bhoyroo, V., & Puchooa, D. (2019) · Microbiology Research · doi:10.4081/mr.2019.7936 · Cited by 45
Current perspectives on integrated approaches to enhance lipid accumulation in microalgae
Rawat, J., Gupta, P. K., Pandit, S., Prasad, R., & Pande, V. (2021) · 3 Biotech · doi:10.1007/s13205-021-02851-3 · Cited by 46
Powerful tools for productivity improvements in microalgal production
Sun, H., Wu, T., Chen, S. H. Y., Ren, Y., Yang, S., Huang, J., Mou, H., & Chen, F. (2021) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2021.111609 · Cited by 42
Inducible expression of Haematococcus oil globule protein in the diatom Phaeodactylum tricornutum : Association with lipid droplets and enhancement of TAG accumulation under nitrogen starvation
Shemesh, Z., Leu, S., Khozin‐Goldberg, I., Didi‐Cohen, S., Zarka, A., & Boussiba, S. (2016) · Algal Research · doi:10.1016/j.algal.2016.07.002 · Cited by 41
Overproduction of Bioactive Algal Chrysolaminarin by the Critical Carbon Flux Regulator Phosphoglucomutase
Yang, Y., Li, D., Chen, T., Hao, T.-B., Balamurugan, S., Yang, W., Liu, J., & Li, H. (2018) · Biotechnology Journal · doi:10.1002/biot.201800220 · Cited by 40
Self-citation
Bio‐engineering of microalgae: Challenges and future prospects toward industrial and environmental applications
Muthukrishnan, L. (2022) · Journal of Basic Microbiology · doi:10.1002/jobm.202100417 · Cited by 41
Molecular exploration of algal interaction between the diatom Phaeodactylum tricornutum and the dinoflagellate Alexandrium tamarense
Zheng, J.-W., Li, D., Yang, L., Chen, J., Liang, J., Zhang, L., Yang, W., Liu, J., Lü, S., & Li, H. (2016) · Algal Research · doi:10.1016/j.algal.2016.04.019 · Cited by 43
Self-citation
Orienting biodiesel production towards sustainability and circularity by tailoring the feedstock and processes
Sreeharsha, R. V., Dubey, N., & Mohan, S. V. (2023) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2023.137526 · Cited by 41
Carbon Orientation in the Diatom Phaeodactylum tricornutum: The Effects of Carbon Limitation and Photon Flux Density
Heydarizadeh, P., Veidl, B., Huang, B., Lukomska, E., Wielgosz‐Collin, G., Couzinet‐Mossion, A., Bougaran, G., Marchand, J., & Schoefs, B. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00471 · Cited by 38
Glucose‐6‐Phosphate Dehydrogenase from the Oleaginous Microalga Nannochloropsis Uncovers Its Potential Role in Promoting Lipogenesis
Xue, J., Chen, T., Zheng, J., Balamurugan, S., Liu, Y., Yang, W., Liu, J., & Li, H. (2019) · Biotechnology Journal · doi:10.1002/biot.201900135 · Cited by 36
Self-citation
Emerging Technologies for Enhancing Microalgae Biofuel Production: Recent Progress, Barriers, and Limitations
Olabi, A. G., Alami, A. H., Alasad, S., Aljaghoub, H., Sayed, E. T., Shehata, N., Rezk, H., & Abdelkareem, M. A. (2022) · Fermentation · doi:10.3390/fermentation8110649 · Cited by 37
Overexpression of malic enzyme isoform 2 in Chlamydomonas reinhardtii PTS42 increases lipid production
Kim, J., Kwak, H. S., Sim, S. J., & Jin, E. (2019) · Bioresource Technology Reports · doi:10.1016/j.biteb.2019.100239 · Cited by 34
Converting carbon dioxide to high value‐added products: Microalgae‐based green biomanufacturing
Sun, Z., Chen, H., Sun, L., & Wang, Q. (2023) · GCB Bioenergy · doi:10.1111/gcbb.13031 · Cited by 35
Recent Trends in Strain Improvement for Production of Biofuels From Microalgae
Bharadwaj, S. V. V., Ram, S., Pancha, I., & Mishra, S. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-817536-1.00014-x · Cited by 35
Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances
Shi, Q., Chen, C., Zhang, W., Wu, P., Sun, M., Wu, H. C., Wu, H., Fu, P., & Fan, J. (2021) · Journal of Applied Phycology · doi:10.1007/s10811-020-02350-7 · Cited by 34
Biotechnology in Future Food Lipids: Opportunities and Challenges
Xu, Q., Tang, Q., Xu, Y., Wu, J., Mao, X., Li, F., Wang, S., & Wang, Y. (2023) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-060721-024353 · Cited by 34
Implication of Industrial Waste for Biomass and Lipid Production in Chlorella minutissima Under Autotrophic, Heterotrophic, and Mixotrophic Grown Conditions
Dubey, K. K., Kumar, S., Dixit, D., Kumar, P., Kumar, D., Jawed, A., & Haque, S. (2015) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-015-1663-6 · Cited by 35
Targeted genome editing in algae using CRISPR/Cas9
Tanwar, A., Sharma, S., & Kumar, S. B. (2018) · Indian Journal of Plant Physiology · doi:10.1007/s40502-018-0423-3 · Cited by 33
A comprehensive review on the heterotrophic production of bioactive compounds by microalgae
Lu, X., Zhao, W., Wang, J., He, Y., Yang, S., & Sun, H. (2024) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-024-03892-5 · Cited by 32
Nuclear transformation and functional gene expression in the oleaginous microalga Monoraphidium neglectum
Jaeger, D., Hübner, W., Huser, T., Mussgnug, J. H., & Kruse, O. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.03.011 · Cited by 31
13C Metabolic Flux Analysis of Enhanced Lipid Accumulation Modulated by Ethanolamine in Crypthecodinium cohnii
Cui, J., Diao, J., Sun, T., Shi, M., Liu, L., Wang, F., Chen, L., & Zhang, W. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00956 · Cited by 31
Long‐term exposure to increasing temperature can offset predicted losses in marine food quality (fatty acids) caused by ocean warming
Jin, P., González, G., & Agustı́, S. (2020) · Evolutionary Applications · doi:10.1111/eva.13059 · Cited by 29
Transgenicism in algae: Challenges in compatibility, global scenario and future prospects for next generation biofuel production
Bharathiraja, B., Iyyappan, J., Gopinath, M., Jayamuthunagai, J., & Praveenkumar, R. (2021) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2021.111829 · Cited by 32
Latest Expansions in Lipid Enhancement of Microalgae for Biodiesel Production: An Update
Rawat, J., Gupta, P. K., Pandit, S., Priya, K., Agarwal, D., Pant, M., Thakur, V. K., & Pande, V. (2022) · Energies · doi:10.3390/en15041550 · Cited by 30
Fast and cost-effective preparation of plant cells for scanning electron microscopy (SEM) analysis
Golinejad, S., & Mirjalili, M. H. (2020) · Analytical Biochemistry · doi:10.1016/j.ab.2020.113920 · Cited by 29
Enhancement of lipid production and nutrient removal of Monoraphidium sp. FXY-10 by combined melatonin and molasses wastewater treatment
Dong, X., Zhao, Y., Li, T., Huang, L., Zhao, P., Xu, J., Ma, H., & Yu, X. (2019) · Journal of the Taiwan Institute of Chemical Engineers · doi:10.1016/j.jtice.2019.03.010 · Cited by 29
Recent Advances in Feedstock and Lipase Research and Development towards Commercialization of Enzymatic Biodiesel
Zambare, V., Patankar, R. S., Bhusare, B. P., & Christopher, L. P. (2021) · Processes · doi:10.3390/pr9101743 · Cited by 29
Advanced biotechnology in biorefinery: a new insight into municipal waste management to the production of high-value products
Haddadi, M. H., Aiyelabegan, H. T., & Negahdari, B. (2017) · International Journal of Environmental Science and Technology · doi:10.1007/s13762-017-1424-x · Cited by 27
Sustainable Microalgae Cultivation: A Comprehensive Review of Open and Enclosed Systems for Biofuel and High Value Compound Production
Zhang, Q., Guan, Y., Zhang, Z., Dong, S., Yuan, T., Ruan, Z., & Chen, M. (2024) · E3S Web of Conferences · doi:10.1051/e3sconf/202457701008 · Cited by 28
Improving Lipid Content in the Diatom Phaeodactylum tricornutum by the Knockdown of the Enoyl-CoA Hydratase Using CRISPR Interference
Guo, W., Weng, Y., Ma, W., Chang, C., Gao, Y., Huang, X., & Zhang, F. (2024) · Current Issues in Molecular Biology · doi:10.3390/cimb46100649 · Cited by 29
Progress of metabolic engineering for the production of eicosapentaenoic acid
Jia, Y.-L., Geng, S., Du, F., Xu, Y., Wang, L., Sun, X., Wang, Q., & Li, Q. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1971621 · Cited by 28
The Heat Shock Transcription Factor PtHSF1 Mediates Triacylglycerol and Fucoxanthin Synthesis by Regulating the Expression of GPAT3 and DXS in Phaeodactylum tricornutum
Song, J., Zhao, H., Zhang, L., Li, Z., Han, J., Zhou, C., Xu, J., Li, X., & Yan, X. (2023) · Plant and Cell Physiology · doi:10.1093/pcp/pcad023 · Cited by 26
Biorefinery of Microalgae for Nonfuel Products
Aslam, A., Fazal, T., Zaman, Q. U., Shan, A., Rehman, F., Iqbal, J., Rashid, N., & Ur Rehman, M. S. (2020) · Microalgae Cultivation for Biofuels Production · doi:10.1016/b978-0-12-817536-1.00013-8 · Cited by 25
Heat shock transcription factor-mediated thermal tolerance and cell size plasticity in marine diatoms
Huang, D., Cheng, C.-Q., Zhang, H.-Y., Huang, Y., Li, S., Huang, Y.-T., Huang, X., Pei, L.-L., Luo, Z., Zou, L., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-58547-2 · Cited by 27
Self-citation
A comprehensive review on enhanced production of microbial lipids for high-value applications
Gupta, R., Banerjee, S., Pandit, S., Gupta, P. K., Mathuriya, A. S., Kumar, S., Lahiri, D., Nag, M., Ray, R. R., & Joshi, S. J. (2021) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-021-02008-5 · Cited by 28
Utilization of lipidic food waste as low-cost nutrients for enhancing the potentiality of biofuel production from engineered diatom under temperature variations
Yang, Y., Ye, G., Wang, H., Li, H.-Y., Lin, C. S. K., Zheng, X.-F., Pugazhendhi, A., & Wang, X. (2023) · Bioresource Technology · doi:10.1016/j.biortech.2023.129611 · Cited by 24
Self-citation
Purple acid phosphatase promoted hydrolysis of organophosphate pesticides in microalgae
Wang, X., He, G.-H., Wang, Z., Xu, H., Mou, J., Qin, Z., Lin, C. S. K., Yang, W., Zhang, Y., & Li, H. (2023) · Environmental Science and Ecotechnology · doi:10.1016/j.ese.2023.100318 · Cited by 25
Self-citation
Potentiation of concurrent expression of lipogenic genes by novel strong promoters in the oleaginous microalga Phaeodactylum tricornutum
Zou, L., Balamurugan, S., Zhou, T., Chen, J., Li, D., Yang, W., Liu, J., & Li, H. (2019) · Biotechnology and Bioengineering · doi:10.1002/bit.27110 · Cited by 24
Self-citation
The role of diatom glucose-6-phosphate dehydrogenase on lipogenic NADPH supply in green microalgae through plastidial oxidative pentose phosphate pathway
Xue, J., Chen, T., Zheng, J.-W., Balamurugan, S., Cai, J.-X., Liu, Y., Yang, W., Liu, J., & Li, H. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9415-5 · Cited by 23
Self-citation
Functional elucidation of hypothetical proteins associated with lipid accumulation: Prioritizing genetic engineering targets for improved algal biofuel production
Sahoo, S., Mahapatra, S. R., Das, N., Parida, B. K., Rath, S., Misra, N., & Suar, M. (2020) · Algal Research · doi:10.1016/j.algal.2020.101887 · Cited by 22
Regulation of malate-pyruvate pathway unifies the adequate provision of metabolic carbon precursors and NADPH in Tetradesmus obliquus
Xue, J., Li, T., Chen, T., Balamurugan, S., Yang, W., & Li, H. (2021) · Algal Research · doi:10.1016/j.algal.2021.102340 · Cited by 20
Self-citation
Focus on the role of synthetic phytohormone for mixotrophic growth and lipid accumulation by Chlorella pyrenoidosa
Yu, J., You, X., Wang, Y., Jin, C., Zhao, Y., & Guo, L. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.136558 · Cited by 21
Algae as a source of renewable energy: opportunities, challenges, and recent developments
Hussain, J., & Rittmann, B. E. (2023) · Sustainable Energy & Fuels · doi:10.1039/d2se01599d · Cited by 22
UV and γ-radiation induced molecular changes for rapid lipid accumulation in Chlorella sorokiniana
Singh, R. D., Sethy, S., Ghosh, S. B., & Srivastava, A. K. (2022) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2022.106493 · Cited by 22
Potential avenue of genetic engineered algal derived bioactive compounds: influencing parameters, challenges and future prospects
Kothari, R., Singh, H. M., Azam, R., Goria, K., Bharti, A., Singh, A., Bajar, S., Pathak, A., Pandey, A., & Tyagi, V. V. (2023) · Phytochemistry Reviews · doi:10.1007/s11101-023-09859-y · Cited by 20
UDP-glucose pyrophosphorylase as a target for regulating carbon flux distribution and antioxidant capacity in Phaeodactylum tricornutum
Zhang, R., Zhu, B., Sun, C., Li, Y., Yang, G., Zhao, Y., & Pan, K. (2023) · Communications Biology · doi:10.1038/s42003-023-05096-3 · Cited by 20
N-acyl homoserine lactones (AHLs) enhanced removal of cadmium and other pollutants by algae-bacteria consortia
Yu, Q., Chen, J., Ye, M., Wei, Y., Zhang, C., & Ge, Y. (2024) · Journal of Environmental Management · doi:10.1016/j.jenvman.2024.121792 · Cited by 20
Biofuel production: tapping into microalgae despite challenges
Sekhon, K. K., Relph, L. E., Armstrong, M. C., & Rahman, P. K. S. M. (2016) · Biofuels · doi:10.1080/17597269.2016.1224290 · Cited by 20
The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae
Molina-Márquez, A., Vilà, M., Vigara, J., Borrero, A., & León, R. (2019) · Metabolites · doi:10.3390/metabo9030049 · Cited by 19
Relationship between acyl-lipid and sterol metabolisms in diatoms
Maréchal, É., & Lupette, J. (2019) · Biochimie · doi:10.1016/j.biochi.2019.07.005 · Cited by 19
The combined effect of nitrogen deprivation and overexpression of malic enzyme gene on growth and lipid accumulation in Phaeodactylum tricornutum
Zhang, R., Zhu, B., Tu, C., Li, Y., Zhao, Y., & Pan, K. (2021) · Journal of Applied Phycology · doi:10.1007/s10811-021-02587-w · Cited by 18
Futuristic opportunities for pretreatment processes in biofuel production from microalgae
Tan, C. H., Low, S. S., Cheah, W. Y., Singh, J., Chai, W. S., Kiong, T. S., & Show, P. L. (2024) · GCB Bioenergy · doi:10.1111/gcbb.13136 · Cited by 18
A review on unit operations, challenges, opportunities, and strategies to improve algal based biodiesel and biorefinery
Kumar, L., Mohan, L., Anand, R., Joshi, V., Chugh, M., & Bharadvaja, N. (2022) · Frontiers in Chemical Engineering · doi:10.3389/fceng.2022.998289 · Cited by 20
Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis
Cui, H., Zhao, C., Xu, W., Zhang, H., Wei, H., Zhu, X., Ji, C., Xue, J., Zhang, C., & Li, R. (2021) · BMC Plant Biology · doi:10.1186/s12870-020-02794-6 · Cited by 19
Self-citation
A Review on Molecular Tools of Microalgal Genetic Transformation and their Application for Overexpression of Different Genes
Rathod, J. P., Gade, R. M., Rathod, D. R., & Dudhare, M. S. (2017) · International Journal of Current Microbiology and Applied Sciences · doi:10.20546/ijcmas.2017.612.373 · Cited by 18
New strategies enhancing feasibility of microalgal cultivations
Caprio, F. D., Altimari, P., & Pagnanelli, F. (2019) · Studies in surface science and catalysis · doi:10.1016/b978-0-444-64337-7.00016-1 · Cited by 17
Hydrogen Sulfide Improves Lipid Accumulation in Nannochloropsis oceanica through Metabolic Regulation of Carbon Allocation and Energy Supply
Cheng, J., Wang, Z., Lu, H., Yang, W., & Fan, Z. (2020) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.9b06748 · Cited by 17
Self-citation
In vitro fucoxanthin production by the Phaeodactylum tricornutum diatom
Bauer, C. M., Schmitz, C., Corrêa, R. G., Herrera, C. M., Ramlov, F., Oliveira, E. R., Pizzato, A., Varela, L. A. C., Cabral, D. Q., Yunes, R. A., et al. (2019) · Studies in natural products chemistry · doi:10.1016/b978-0-12-817901-7.00008-3 · Cited by 18
Heterotrophic modification of Phaeodactylum tricornutum Bohlin
Zhang, W., Zhou, W., Jiang, S. W., Wang, Y., Chen, L., Yang, G., & Liu, T. (2023) · Algal Research · doi:10.1016/j.algal.2023.103137 · Cited by 16
Bioremediation of Catechol and Concurrent Accumulation of Biocompounds by the Microalga Crypthecodinium cohnii
Zou, L., Yao, Y., Wen, F.-F., Zhang, X., Liu, B.-T., Li, D., Yang, Y., Yang, W., Balamurugan, S., & Li, H. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c01814 · Cited by 16
Self-citation
Reconstruction of a Genome-Scale Metabolic Model of Scenedesmus obliquus and Its Application for Lipid Production under Three Trophic Modes
Ray, A., Kundu, P., & Ghosh, A. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00516 · Cited by 16
Oil Extraction Processes in Microalgae
(2017) · Recent advances in renewable energy · doi:10.2174/9781681085227117010011 · Cited by 16
Sustainable Microalgal Biomass for Efficient and Scalable Green Energy Solutions: Fueling Tomorrow
Rathinavel, L., Singh, S., Rai, P. K., Chandra, N., Jothinathan, D., Gaffar, I., Pandey, A. K., Choure, K., Waoo, A. A., Joo, J. C., et al. (2024) · Fuels · doi:10.3390/fuels5040049 · Cited by 15
Characterization of a Haematococcus pluvialis Diacylglycerol Acyltransferase 1 and Its Potential in Unsaturated Fatty Acid-Rich Triacylglycerol Production
Cui, H., Xu, W., Zhu, X., Zhao, C., Cui, Y., Ji, C., Zhang, C., Xue, J., Qin, S., Jia, X., et al. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.771300 · Cited by 15
Self-citation
Aromatic Amino Acids: Exploring Microalgae as a Potential Biofactory
Niraula, A., Danesh, A., Mérindol, N., Meddeb‐Mouelhi, F., & Desgagné‐Penix, I. (2025) · BioTech · doi:10.3390/biotech14010006 · Cited by 14
Chrysolaminarin biosynthesis in the diatom is enhanced by overexpression of 1,6-β-transglycosylase
Yang, Y., Li, D., Balamurugan, S., Wang, X., Yang, W., & Li, H. (2022) · Algal Research · doi:10.1016/j.algal.2022.102817 · Cited by 14
Self-citation
Strategies for genetic modification of microalgae to improve the production efficiency of liquid biofuel
Kossalbayev, B. D., Kakimova, A. B., Sadvakasova, A. K., Bauenova, M. O., Balouch, H., Lyaguta, M. A., Ahmad, F., Kirbayeva, D. K., Özgül, S., & Allakhverdiev, S. I. (2024) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2024.12.306 · Cited by 16
Chemicals and Fuels from Microalgae
Jeon, S., Jeong, B., & Chang, Y. K. (2016) · Springer eBooks · doi:10.1007/978-3-319-31421-1_384-1 · Cited by 14
Exogenous Addition of Putrescine Enhanced Lipid Accumulation in Tetradesmus Obliquus for Increased Biodiesel Productivity
Li, T., Li, J., Sheng, X., Li, B., Wang, W., Xue, Y., Zhang, J., Li, W., Wang, X., Wang, F., et al. (2022) · Renewable Energy · doi:10.1016/j.renene.2023.02.037 · Cited by 13
Self-citation
Development of Titania-Integrated Silica Cell Walls of the Titanium-Resistant Diatom, Fistulifera solaris
Maeda, Y., Niwa, Y., Tang, H., Kisailus, D., Yoshino, T., & Tanaka, T. (2018) · ACS Applied Bio Materials · doi:10.1021/acsabm.8b00520 · Cited by 13
Phloroglucinol Promotes Fucoxanthin Synthesis by Activating the cis -Zeatin and Brassinolide Pathways in Thalassiosira pseudonana
Yan, L., Li, Y., Zhao, H., Zhu, B., Xu, J., Feng, X., Liu, S., Li, X., & Zhou, C. (2022) · Applied and Environmental Microbiology · doi:10.1128/aem.02160-21 · Cited by 13
The first genetic engineered system for ovothiol biosynthesis in diatoms reveals a mitochondrial localization for the sulfoxide synthase OvoA
Russo, M. T., Santin, A., Zuccarotto, A., Leone, S., Palumbo, A., Ferrante, M. I., & Castellano, I. (2023) · Open Biology · doi:10.1098/rsob.220309 · Cited by 13
Bioinformatics-Based Screening Approach for the Identification and Characterization of Lipolytic Enzymes from the Marine Diatom Phaeodactylum tricornutum
Murison, V., Hérault, J., Schoefs, B., Marchand, J., & Ulmann, L. (2023) · Marine Drugs · doi:10.3390/md21020125 · Cited by 13
Biodiesel production from microalgae: progress and biotechnological prospects
Rodríguez‐Núñez, J. R., & Universidad de Guanajuato. Programa de Biotecnología. Celaya, G. (2017) · Hidrobiológica · doi:10.24275/uam/izt/dcbi/hidro/2017v27n3/rodriguez · Cited by 13
Enhanced lipid production in Chlamydomonas reinhardtii: Impact of salt stress synergized with α-lipoic acid
Xing, X., Ying, K., Liu, H., Liu, C., & Zheng, L. (2025) · Food Chemistry · doi:10.1016/j.foodchem.2025.144596 · Cited by 12
Microbial Oils as Nutraceuticals and Animal Feeds
Galán, B., Santos‐Merino, M., Nogales, J., de la Cruz, F., & García, J. L. (2020) · Springer eBooks · doi:10.1007/978-3-030-15147-8_34 · Cited by 12
Microbial Oils as Nutraceuticals and Animal Feeds
Galán, B., Santos‐Merino, M., Nogales, J., de la Cruz, F., & García, J. L. (2018) · Springer eBooks · doi:10.1007/978-3-319-72473-7_34-1 · Cited by 11
Ecology and Diversity of Diatoms in Kuttanadu Paddy Fields in Relation to Soil Regions, Seasons and Paddy-Growth-Stages
Vijayan, D., & Ray, J. G. (2016) · Journal of Plant Studies · doi:10.5539/jps.v5n2p7 · Cited by 12
Neutral lipid and chrysolaminarin metabolic pathways during nitrogen starvation and recovery in the diatom Phaeodactylum tricornutum
Murison, V., Hérault, J., Guinio, S., Côme, M., Loiseau, C., Kwasiborski, A., Marchand, J., & Ulmann, L. (2024) · Algal Research · doi:10.1016/j.algal.2024.103621 · Cited by 11
Biodiesel in circular economy: A perspective study on recent trends and environmental challenges in sustainable energy production
Jan, S., Mishra, A. K., Bhat, M. A., Bhat, M. A., Qyyum, M. A., Rahman, S., & Jan, A. T. (2025) · Renewable Energy · doi:10.1016/j.renene.2025.123840 · Cited by 12

Authors: Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y.
Affiliations: Jinan University (CN)
ORCID: Xue, Jiao
Niu, Ying-Fang
Yang, Wei-Dong
Li, Hong-Ye
Venue: Metabolic Engineering
Publisher: Elsevier BV
Published: 2015-01
Volume: 27
Pages: 1-9
ISSN: 1096-7176 (ISSN-L)
DOI: 10.1016/j.ymben.2014.10.002
OpenAlex: W1986227133
Cited by: 321
References: 51
Citations by year: 2026: 12 · 2025: 20 · 2024: 22 · 2023: 31 · 2022: 30 · 2021: 41 · 2020: 34 · 2019: 36 · 2018: 26 · 2017: 27 · 2016: 29 · 2015: 13
FWCI: 7.59
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-sa
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by-nc-sa/3.0/ (vor)
Locations: Metabolic Engineering (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
Funders: National Science and Technology Support Program (2011BAD14B03)
Science and Technology Project of Guangdong (2009B050600005, 2010B030600005)
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords: Phaeodactylum tricornutum, malic enzyme, neutral lipid accumulation, transgenic microalgae, nitrogen starvation, oil bodies, mitochondrial targeting, pyruvate metabolism, CO2 fixation, microalgae biodiesel, genetic improvement
MeSH terms: Genetic Engineering, Malate Dehydrogenase, Gene Expression, Diatoms, Algal Proteins, Organisms, Genetically Modified, Lipid Metabolism

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks