The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440
Open accessAbstract
Poly(ethylene terephthalate) (PET) is the most abundantly consumed synthetic polyester and accordingly a major source of plastic waste. The development of chemocatalytic approaches for PET depolymerization to monomers offers new options for open-loop upcycling of PET, which can leverage biological transformations to higher-value products. To that end, here we perform four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2IIA3IIBIIA1II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) terephthalate (BHET) hydrolysis to TPA by expression of PETase and MHETase from Ideonella sakaiensis, and (iv) BHET conversion to a performance-advantaged bioproduct, β-ketoadipic acid (βKA) by deletion of pcaIJ. Using this strain, we demonstrate production of 15.1 g/L βKA from BHET at 76% molar yield in bioreactors and conversion of catalytically depolymerized PET to βKA. Overall, this work highlights the potential of tandem catalytic deconstruction and biological conversion as a means to upcycle waste PET.
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Cites
A bacterium that degrades and assimilates poly(ethylene terephthalate)
Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., Toyohara, K., Miyamoto, K., Kimura, Y., & Oda, K. (2016) · Science · doi:10.1126/science.aad6359 · Cited by 3,367
Mechanical and chemical recycling of solid plastic waste
Ragaert, K., Delva, L., & Van Geem, K. (2017) · Waste Management · doi:10.1016/j.wasman.2017.07.044 · Cited by 3,276
An engineered PET depolymerase to break down and recycle plastic bottles
Tournier, V., Topham, C. M., Gilles, A., David, B., Folgoas, C., Moya-Leclair, E., Kamionka, E., Desrousseaux, M.-L., Texier, H., Gavalda, S., et al. (2020) · Nature · doi:10.1038/s41586-020-2149-4 · Cited by 2,176
Chemical Recycling of Poly(ethylene terephthalate)
Paszun, D., & Spychaj, T. (1997) · Industrial & Engineering Chemistry Research · doi:10.1021/ie960563c · Cited by 661
Manufacturing energy and greenhouse gas emissions associated with plastics consumption
Nicholson, S. R., Rorrer, N. A., Carpenter, A. C., & Beckham, G. T. (2021) · Joule · doi:10.1016/j.joule.2020.12.027 · Cited by 421
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Cited by
Mechanism-Based Design of Efficient PET Hydrolases
Wei, R., von Haugwitz, G., Pfaff, L., Mican, J., Badenhorst, C. P. S., Liu, W., Weber, G., Austin, H. P., Bednar, D., Damborsky, J., et al. (2022) · ACS Catalysis · doi:10.1021/acscatal.1c05856 · Cited by 284
Schaerer, L. G., Wu, R., Putman, L. I., Pearce, J. M., Lu, T., Shonnard, D. R., Ong, R. G., & Techtmann, S. M. (2023) · Trends in Biotechnology · doi:10.1016/j.tibtech.2022.06.012 · Cited by 52
(2025) · doi:10.1186/s13036-025-00568-y · Cited by 4
Metadata
- Authors
- Werner, A. Z., Clare, R., Mand, T. D., Pardo, I., Ramirez, K. J., Haugen, S. J., Bratti, F., Dexter, G. N., Elmore, J. R., Huenemann, J. D., Peabody, G. L., Johnson, C. W., Rorrer, N. A., Salvachúa, D., Guss, A. M., & Beckham, G. T.
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2021-09
- Volume
- 67
- Pages
- 250-261
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W3180069095
- Cited by
- 207
- References
- 5
- FWCI
- 7.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Werner, A. Z., Clare, R., Mand, T. D., Pardo, I., Ramirez, K. J., Haugen, S. J., Bratti, F., Dexter, G. N., Elmore, J. R., Huenemann, J. D., Peabody, G. L., Johnson, C. W., Rorrer, N. A., Salvachúa, D., Guss, A. M., & Beckham, G. T. (2021). Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440. Metabolic Engineering. https://doi.org/10.1016/j.ymben.2021.07.005
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
