The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans
Open accessAbstract
The ability to introduce targeted edits in the genome of model organisms is revolutionizing the field of genetics. State-of-the-art methods for precision genome editing use RNA-guided endonucleases to create double-strand breaks (DSBs) and DNA templates containing the edits to repair the DSBs. Following this strategy, we have developed a protocol to create precise edits in the C. elegans genome. The protocol takes advantage of two innovations to improve editing efficiency: direct injection of CRISPR-Cas9 ribonucleoprotein complexes and use of linear DNAs with short homology arms as repair templates. The protocol requires no cloning or selection, and can be used to generate base and gene-size edits in just 4days. Point mutations, insertions, deletions and gene replacements can all be created using the same experimental pipeline.
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Cites
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Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation
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Self-citationCRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
Dickinson, D. J., & Goldstein, B. (2016) · Genetics · doi:10.1534/genetics.115.182162 · Cited by 332
Renaud, J.-B., Boix, C., Charpentier, M., De Cian, A., Cochennec, J., Duvernois-Berthet, E., Perrouault, L., Tesson, L., Edouard, J., Thinard, R., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.02.018 · Cited by 301
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Self-citation
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Neural signal propagation atlas of Caenorhabditis elegans
Randi, F., Sharma, A. K., Dvali, S., & Leifer, A. M. (2023) · Nature · doi:10.1038/s41586-023-06683-4 · Cited by 153
piRNA processing by a trimeric Schlafen-domain nuclease
Podvalnaya, N., Bronkhorst, A. W., Lichtenberger, R., Hellmann, S., Nischwitz, E., Falk, T., Karaulanov, E., Butter, F., Falk, S., & Ketting, R. F. (2023) · Nature · doi:10.1038/s41586-023-06588-2 · Cited by 47
BUB-1-bound PLK-1 directs CDC-20 kinetochore recruitment to ensure timely embryonic mitoses
Houston, J., Ohta, M., Gómez-Cavazos, J. S., Deep, A., Corbett, K. D., Oegema, K., Lara-Gonzalez, P., Kim, T., & Desai, A. (2023) · Current Biology · doi:10.1016/j.cub.2023.04.021 · Cited by 13
Metadata
- Authors
- Paix, A., Folkmann, A., & Seydoux, G.
- Venue
- Methods
- Publisher
- Elsevier BV
- Published
- 2017-05
- Volume
- 121-122
- Pages
- 86-93
- ISSN
- 1046-2023 (ISSN-L)
- OpenAlex
- W2605356232
- Cited by
- 256
- References
- 23
- FWCI
- 6.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Paix, A., Folkmann, A., & Seydoux, G. (2017). Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans. Methods. https://doi.org/10.1016/j.ymeth.2017.03.023
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