The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases
Open accessAbstract
Genome editing has now been reported in many systems using TALEN and CRISPR-Cas9 nucleases. Precise mutations can be introduced during homology-directed repair with donor DNA carrying the wanted sequence edit, but efficiency is usually lower than for gene knockout and optimal strategies have not been extensively investigated. Here, we show that using phosphorothioate-modified oligonucleotides strongly enhances genome editing efficiency of single-stranded oligonucleotide donors in cultured cells. In addition, it provides better design flexibility, allowing insertions more than 100 bp long. Despite previous reports of phosphorothioate-modified oligonucleotide toxicity, clones of edited cells are readily isolated and targeted sequence insertions are achieved in rats and mice with very high frequency, allowing for homozygous loxP site insertion at the mouse ROSA locus in particular. Finally, when detected, imprecise knockin events exhibit indels that are asymmetrically positioned, consistent with genome editing taking place by two steps of single-strand annealing.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
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Genome engineering using the CRISPR-Cas9 system
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GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
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Engineered CRISPR-Cas9 nucleases with altered PAM specificities
Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J.-R. J., et al. (2015) · Nature · doi:10.1038/nature14592 · Cited by 1,624
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A Platform for Rapid Exploration of Aging and Diseases in a Naturally Short-Lived Vertebrate
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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
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Nuclear sensing of breaks in mitochondrial DNA enhances immune surveillance
Tigano, M., Vargas, D. C., Tremblay-Belzile, S., Fu, Y., & Sfeir, A. (2021) · Nature · doi:10.1038/s41586-021-03269-w · Cited by 327
Methodologies for Improving HDR Efficiency
Liu, M., Rehman, S., Tang, X., Gu, K., Fan, Q., Chen, D., & Ma, W. (2019) · Frontiers in Genetics · doi:10.3389/fgene.2018.00691 · Cited by 323
Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans
Paix, A., Folkmann, A., & Seydoux, G. (2017) · Methods · doi:10.1016/j.ymeth.2017.03.023 · Cited by 256
Carlson-Stevermer, J., Abdeen, A. A., Kohlenberg, L., Goedland, M., Molugu, K., Lou, M., & Saha, K. (2017) · Nature Communications · doi:10.1038/s41467-017-01875-9 · Cited by 170
Metadata
- Authors
- Renaud, J.-B., Boix, C., Charpentier, M., De Cian, A., Cochennec, J., Duvernois-Berthet, E., Perrouault, L., Tesson, L., Edouard, J., Thinard, R., Cherifi, Y., Menoret, S., Fontanière, S., de Crozé, N., Fraichard, A., Sohm, F., Anegon, I., Concordet, J.-P., & Giovannangeli, C.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2016-03
- Volume
- 14
- Issue
- 9
- Pages
- 2263-2272
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2285096912
- Cited by
- 301
- References
- 7
- FWCI
- 17.76
- Type
- journal article
- Language
- en
- Open access
- gold
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- The paper
- Renaud, J.-B., Boix, C., Charpentier, M., De Cian, A., Cochennec, J., Duvernois-Berthet, E., Perrouault, L., Tesson, L., Edouard, J., Thinard, R., Cherifi, Y., Menoret, S., Fontanière, S., de Crozé, N., Fraichard, A., Sohm, F., Anegon, I., Concordet, J.-P., & Giovannangeli, C. (2016). Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases. Cell Reports. https://doi.org/10.1016/j.celrep.2016.02.018
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