The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
The Internalization Route Resulting in Successful Gene Expression Depends on both Cell Line and Polyethylenimine Polyplex Type
Open accessAbstract
Understanding cellular uptake and intracellular processing of nonviral gene delivery systems is a key aspect in developing more efficient vectors. In this study, the impact of clathrin- and caveolae/lipid-raft-dependent endocytosis on cell entry and overall transfection efficiency of polyethylenimine (PEI) polyplexes was evaluated. Most remarkably, the internalization pathway mediating successful transfection depended on both cell type and polyplex type applied. Colocalization studies with transferrin and cholera toxin B revealed that at least two specific endocytosis pathways--the clathrin-dependent and the lipid-raft-dependent--mediated cellular uptake of PEI polyplexes. With the help of specific uptake inhibitors (chlorpromazine and filipin III), cell-line-dependent variations regarding the route of successful transfection were observed (HUH-7, COS-7, HeLa). In COS-7 cells, the clathrin-dependent pathway was the main contributor to the transfection process. In HUH-7 cells, gene transfer by linear PEI polyplexes succeeded mainly via the clathrin-dependent route, whereas transfection by branched PEI polyplexes was mediated by both pathways. In HeLa cells, both pathways were able to mediate successful gene delivery. However, the lipid-raft-dependent pathway was more relevant. The study also revealed that the concentration window between specific inhibitory function and nonspecific toxicity of the uptake inhibitors was very narrow.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Boussif, O., Lezoualc'h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., & Behr, J. P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,247
REJMAN, J., OBERLE, V., ZUHORN, I. S., & HOEKSTRA, D. (2004) · Biochemical Journal · doi:10.1042/bj20031253 · Cited by 2,864
Caveolin, a protein component of caveolae membrane coats
Rothberg, K. G., Heuser, J. E., Donzell, W. C., Ying, Y.-S., Glenney, J. R., & Anderson, R. G. W. (1992) · Cell · doi:10.1016/0092-8674(92)90143-z · Cited by 2,258
Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER
Pelkmans, L., Kartenbeck, J., & Helenius, A. (2001) · Nature Cell Biology · doi:10.1038/35074539 · Cited by 1,235
Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation.
Wang, L. H., Rothberg, K. G., & Anderson, R. G. (1993) · The Journal of cell biology · doi:10.1083/jcb.123.5.1107 · Cited by 1,177
Chloride Accumulation and Swelling in Endosomes Enhances DNA Transfer by Polyamine-DNA Polyplexes
Sonawane, N. D., Szoka, F. C., & Verkman, A. S. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m308643200 · Cited by 1,081
Rejman, J., Bragonzi, A., & Conese, M. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2005.03.038 · Cited by 851
Evidence for the role of proteoglycans in cation-mediated gene transfer.
Mislick, K. A., & Baldeschwieler, J. D. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.22.12349 · Cited by 772
Pelkmans, L., & Helenius, A. (2002) · Traffic · doi:10.1034/j.1600-0854.2002.30501.x · Cited by 747
Orlandi, P. A., & Fishman, P. H. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.141.4.905 · Cited by 722
Cited by
Sahay, G., Alakhova, D. Y., & Kabanov, A. V. (2010) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.01.036 · Cited by 2,181
Xia, T., Kovochich, M., Liong, M., Zink, J. I., & Nel, A. E. (2008) · ACS Nano · doi:10.1021/nn700256c · Cited by 624
Nucleic Acid Therapeutics Using Polyplexes: A Journey of 50 Years (and Beyond)
Lächelt, U., & Wagner, E. (2015) · Chemical Reviews · doi:10.1021/cr5006793 · Cited by 600
Self-citationThe great escape: how cationic polyplexes overcome the endosomal barrier
Bus, T., Traeger, A., & Schubert, U. S. (2018) · Journal of Materials Chemistry B · doi:10.1039/c8tb00967h · Cited by 409
Polymeric Delivery of Therapeutic Nucleic Acids
Kumar, R., Santa Chalarca, C. F., Bockman, M. R., Bruggen, C. V., Grimme, C. J., Dalal, R. J., Hanson, M. G., Hexum, J. K., & Reineke, T. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00997 · Cited by 365
Metadata
- Authors
- von Gersdorff, K., Sanders, N. N., Vandenbroucke, R., De Smedt, S. C., Wagner, E., & Ogris, M.
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2006-11
- Volume
- 14
- Issue
- 5
- Pages
- 745-753
- ISSN
- 1525-0016 (ISSN-L)
- OpenAlex
- W2016568056
- Cited by
- 314
- References
- 33
- FWCI
- 6.02
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The Internalization Route Resulting in Successful Gene Expression Depends on both Cell Line and Polyethylenimine Polyplex Type. https://app.cochat.ai/papers/10.1016/j.ymthe.2006.07.006
- The paper
- von Gersdorff, K., Sanders, N. N., Vandenbroucke, R., De Smedt, S. C., Wagner, E., & Ogris, M. (2006). The Internalization Route Resulting in Successful Gene Expression Depends on both Cell Line and Polyethylenimine Polyplex Type. Molecular Therapy. https://doi.org/10.1016/j.ymthe.2006.07.006
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
