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Cited by 209Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsDNA and Nucleic Acid Chemistry

Telomere Length Assessment in Human Archival Tissues

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Meeker, Alan K., Gage, Wesley R., Hicks, Jessica L., Simon, Inpakala, Coffman, Jonathan R., Platz, Elizabeth A., March, Gerrun E., De Marzo, Angelo M.·2002·The American Journal of Pathology
DOI 10.1016/s0002-9440(10)62553-9PMC PMC1867217
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Abstract

A method was developed to assess human telomere lengths at the individual cell level in tissue sections from standard formalin-fixed paraffin-embedded tissues. We coupled this method with immunofluorescence to allow the simultaneous identification of specific cell types. Validation of this in situ quantification method showed excellent agreement with the commonly used telomere repeat fragment-Southern blot method. The assay requires very few cells ( approximately 10 to 15). Thus, small tissue samples, including clinical biopsies, can be easily accommodated. In addition, the cells under study need not be actively cycling and there is no requirement for tissue disaggregation or cell culture. This method provides a more accurate assessment of telomere lengths than Southern blotting because confounding contributions from undesired cell types within tissue samples are avoided. Using this technique, we were able to perform the first comparison of relative telomere lengths in matched tumor versus normal epithelial cells within archival human prostate tissues.

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  • Early Southern blot experiments showed significantly shorter telomeres in breast and colon cancers than in adjacent normal tissue.

    “Early experiments relying on Southern blot analysis showed significantly shorter telomeres in breast and colon cancers compared with adjacent normal tissue samples (120-122).”

    From Telomeres and telomerase in cancer (2010) · cites this paper as [121]

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Cited by

Sorted by citations

Authors
Meeker, A. K., Gage, W. R., Hicks, J. L., Simon, I., Coffman, J. R., Platz, E. A., March, G. E., & De Marzo, A. M.
Affiliations
  • Johns Hopkins University (US)
  • MathWorks (United States) (US)
ORCID
Venue
The American Journal of Pathology
Publisher
Elsevier BV
Published
2002-04
Volume
160
Issue
4
Pages
1259-1268
ISSN
0002-9440 (ISSN-L)
DOI
10.1016/s0002-9440(10)62553-9
PMC
PMC1867217
OpenAlex
W1517501567
Cited by
209
References
17
Citations by year
2024: 6 · 2023: 5 · 2022: 6 · 2021: 6 · 2020: 6 · 2019: 9 · 2018: 6 · 2017: 11 · 2016: 11 · 2015: 8 · 2014: 15 · 2013: 8 · 2012: 7
FWCI
6.33
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • American Journal Of Pathology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Charlotte Geyer Foundation
  • National Institutes of Health (5k08ca078588-02)
  • National Cancer Institute (CA84997, R01CA084997, P50CA058236, K08CA78588)
  • National Institute of Diabetes and Digestive and Kidney Diseases (T32DK007552, DK-07552)
Topics
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
telomere length, formalin-fixed paraffin-embedded tissue, immunofluorescence, prostate cancer, archival tissue
MeSH terms
Animals, Cell Line, Dogs, Fluorescent Antibody Technique, Humans, Male, Telomere, In Situ Hybridization, Fluorescence, Mice, Rats

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