The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Mechanism of cell transfection with plasmid/chitosan complexes
Open accessAbstract
Chitosan is useful as a non-viral vector for gene delivery. Although there are several reports supporting the use of chitosan for gene delivery, studies regarding effects on transfection and the chitosan-specific transfection mechanism remain insufficient. In this report, the level of expression with plasmid/chitosan was observed to be no less than that with plasmid/lipofectin complexes in SOJ cells. The transfection mechanism of plasmid/chitosan complexes as well as the relationship between transfection activity and cell uptake was analyzed by using fluorescein isothiocyanate-labeled plasmid and Texas Red-labeled chitosan. In regard to effects on transfection, there were several factors to affect transfection activity and cell uptake, for example: the molecular mass of chitosan, stoichiometry of complex, as well as serum concentration and pH of transfection medium. The level of transfection with plasmid/chitosan complexes was found to be highest when the molecular mass of chitosan was 40 or 84 kDa, ratio of chitosan nitrogen to DNA phosphate (N/P ratio) was 5, and transfection medium contained 10% serum at pH 7.0. We also investigated the transfection mechanism, and found that plasmid/chitosan complexes most likely condense to form large aggregates (5-8 microm), which absorb to the cell surface. After this, plasmid/chitosan complexes are endocytosed, and possibly released from endosomes due to swelling of lysosomal in addition to swelling of plasmid/chitosan complex, causing the endosome to rupture. Finally, complexes were also observed to accumulate in the nucleus using a confocal laser scanning microscope.
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Cited by
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Nonviral vectors for gene delivery
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Biodegradation, biodistribution and toxicity of chitosan
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Natural Polymers for Gene Delivery and Tissue Engineering
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RNA Interference in Vitro and in Vivo Using a Novel Chitosan/siRNA Nanoparticle System
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Metadata
- Authors
- Ishii, T., Okahata, Y., & Sato, T.
- Venue
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Publisher
- Elsevier BV
- Published
- 2001-09
- Volume
- 1514
- Issue
- 1
- Pages
- 51-64
- ISSN
- 0005-2736 (ISSN-L)
- OpenAlex
- W2092753252
- Cited by
- 323
- References
- 30
- FWCI
- 3.04
- Type
- journal article
- Language
- en
- Open access
- bronze
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