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Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.

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Behr, J P, Demeneix, B, Loeffler, J P, Perez-Mutul, J·1989·Proceedings of the National Academy of Sciences

Abstract

A general and efficient transfection procedure, based on compacted lipopolyamine-coated plasmids, has been developed. The active species is obtained by simple addition of excess synthetic lipospermine solution to the DNA and binds within minutes to the cell membrane. This technique has been developed on endocrine cells of the intermediate lobe of the pituitary as a general tool for physiological work on primary cells; it is not toxic and does not interfere with physiological regulations in melanotrope cells. A variety of eukaryotic cell cultures also have been transfected with success for transient and stable expression.

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Metadata

Authors
Behr, J. P., Demeneix, B., Loeffler, J. P., & Perez-Mutul, J.
Venue
Proceedings of the National Academy of Sciences
Published
1989-09
Volume
86
Issue
18
Pages
6982-6986
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1991303534
Cited by
719
References
0
FWCI
2.85
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA. https://app.cochat.ai/papers/10.1073/pnas.86.18.6982
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Behr, J. P., Demeneix, B., Loeffler, J. P., & Perez-Mutul, J. (1989). Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.18.6982

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.