The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Nonresonant Confocal Raman Imaging of DNA and Protein Distribution in Apoptotic Cells
Open accessAbstract
Nonresonant confocal Raman imaging has been used to map the DNA and the protein distributions in individual single human cells. The images are obtained on an improved homebuilt confocal Raman microscope. After statistical analysis, using singular value decomposition, the Raman images are reconstructed from the spectra covering the fingerprint region. The data are obtained at a step interval of approximately 250 nm and cover a field from 8- to 15- micro m square in size. Dwell times at each pixel are between 0.5 and 2 s, depending on the nature and the state of the cell under investigation. High quality nonresonant Raman images can only be obtained under these conditions using continuous wave high laser powers between 60 and 120 mW. We will present evidence that these laser powers can still safely be used to recover the chemical distributions in fixed cells. The developed Raman imaging method is used to image directly, i.e., without prior labeling, the nucleotide condensation and the protein distribution in the so-called nuclear fragments of apoptotic HeLa cells. In the control (nonapoptotic) HeLa cells, we show, for the first time by Raman microspectroscopy, the presence of the RNA in a cell nucleus.
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Cites
Golub, G. (2012) · doi:10.56021/9781421407944 · Cited by 34,505
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Majno, G., & Joris, I. (1995) · PubMed · Cited by 3,306
Apoptosis. Its significance in cancer and cancer Therapy
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Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
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Biological Applications of Raman Spectroscopy
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Studying single living cells and chromosomes by confocal Raman microspectroscopy
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Cited by
Label-free DNA imaging in vivo with stimulated Raman scattering microscopy
Lu, F.-K., Basu, S., Igras, V., Hoang, M. P., Ji, M., Fu, D., Holtom, G. R., Neel, V. A., Freudiger, C. W., Fisher, D. E., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1515121112 · Cited by 302
Raman spectroscopy: an evolving technique for live cell studies
Smith, R., Wright, K. L., & Ashton, L. (2016) · The Analyst · doi:10.1039/c6an00152a · Cited by 285
Raman and CARS microspectroscopy of cells and tissues
Krafft, C., Dietzek, B., & Popp, J. (2009) · The Analyst · doi:10.1039/b822354h · Cited by 271
Label‐Free Live‐Cell Imaging of Nucleic Acids Using Stimulated Raman Scattering Microscopy
Zhang, X., Roeffaers, M. B. J., Basu, S., Daniele, J. R., Fu, D., Freudiger, C. W., Holtom, G. R., & Xie, X. S. (2012) · ChemPhysChem · doi:10.1002/cphc.201100890 · Cited by 171
Metadata
- Authors
- Uzunbajakava, N., Lenferink, A., Kraan, Y., Volokhina, E., Vrensen, G., Greve, J., & Otto, C.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2003-06
- Volume
- 84
- Issue
- 6
- Pages
- 3968-3981
- ISSN
- 0006-3495 (ISSN-L)
- OpenAlex
- W2091595316
- Cited by
- 347
- References
- 40
- FWCI
- 8.42
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Uzunbajakava, N., Lenferink, A., Kraan, Y., Volokhina, E., Vrensen, G., Greve, J., & Otto, C. (2003). Nonresonant Confocal Raman Imaging of DNA and Protein Distribution in Apoptotic Cells. Biophysical Journal. https://doi.org/10.1016/s0006-3495(03)75124-8
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