Monte Carlo simulation of miniature endplate current generation in the vertebrate neuromuscular junction
Open accessAbstract
A Monte Carlo method for modeling the neuromuscular junction is described in which the three-dimensional structure of the synapse can be specified. Complexities can be introduced into the acetylcholine kinetic model used with only a small increase in computing time. The Monte Carlo technique is shown to be superior to differential equation modeling methods (although less accurate) if a three-dimensional representation of synaptic geometry is desired. The conceptual development of the model is presented and the accuracy estimated. The consequences of manipulations such as varying the spacing of secondary synaptic folds or that between the release of multiple quantal packets of acetylcholine, are also presented. Increasing the spacing between folds increases peak current. Decreased spacing of adjacent quantal release sites increases the potentiation of peak current.
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Most simulations of synaptic transmission conclude that ACh remains near receptors for <1 ms.
“Therefore, most simulations of synaptic transmission conclude that ACh remains near receptors for <1 ms (37,38).”
From Nicotine is a selective pharmacological chaperone of acetylcholine receptor number and stoichiometry. Implications for drug discovery (2009) · cites this paper as [38]
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- Authors
- Bartol, T. M., Land, B. R., Salpeter, E. E., & Salpeter, M. M.
- Affiliations
- Cornell University (US)
- ORCID
- Bartol, T.M.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 1991-06
- Volume
- 59
- Issue
- 6
- Pages
- 1290-1307
- ISSN
- 0006-3495 (ISSN-L)
- PMC
- PMC1281209
- OpenAlex
- W2024048296
- Cited by
- 217
- References
- 25
- Citations by year
- 2024: 16 · 2023: 21 · 2022: 4 · 2021: 5 · 2019: 2 · 2018: 5 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 6 · 2013: 5 · 2012: 5
- FWCI
- 2.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biophysical Journal (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS 09315)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- neuromuscular junction, miniature endplate currents, acetylcholine release, Monte Carlo simulation
- MeSH terms
- Acetylcholine, Algorithms, Animals, Anura, Biophysics, Computer Simulation, Electrophysiology, Kinetics, Microscopy, Electron, Models, Neurological, Monte Carlo Method, Motor Endplate, Neuromuscular Junction, Receptors, Cholinergic, Biophysical Phenomena
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