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Cited by 111Advanced Glycation End Products researchSystemic Lupus Erythematosus ResearchSkin Diseases and Diabetes

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Detection of autoantibodies against advanced glycation endproducts and AGE-immune complexes in serum of patients with diabetes mellitus

Turk, Zdenka, Ljubic, Spomenka, Turk, Nikša, Benko, Bojan·2001·Clinica Chimica Acta
DOI 10.1016/s0009-8981(00)00389-2
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Abstract

Advanced glycation of protein causes their immunogenicity. The evidence that advanced glycation endproducts (AGEs) have antigenic properties has led to a hypothesis that the AGE structure found in vivo may exert an autoimmune response. In the present study, we showed the sera of diabetic patients as well as of nondiabetic individuals to contain autoantibodies to epitopes of AGE structures. Contrary to what might be expected, we observed lower AGE antibody titers in diabetic subjects, and postulated that the antibodies against AGEs form immune complexes in vivo, hampering their determination. The existence of immune complexes containing AGE moiety was established by two independent criteria: (a) serum AGE-immune complexes (AGE-IC) were detected by enzyme-linked immunosorbent assay (ELISA) using an immunochemical bridge; and (b) soluble AGE-IC were precipitated from serum by polyethylene glycol and analyzed. We demonstrated the presence of circulating AGE-IC in sera, predominantly in the sera of diabetic subjects. We also found an inverse correlation between serum AGE level and AGE-IC (r=-0.8, P<0.000), indicating the serum level of AGEs to decline with an increasing presence of AGE-IC. The content of AGE in soluble immune complexes was significantly higher in diabetic patients than in control subjects (3.51+/-1.9 vs. 1.89+/-1.0 microgEq/ml (P<0.00004), and correlated inversely with free antibodies (r=-0.26, P<0.01). Interactions of AGE autoantibodies with AGE as a continuously produced antigen result in the formation of AGE-immune complexes that may play a role in the atherogenic processes.

Overview

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Crossref · 10.1016/s0009-8981(00)00389-2

Cites

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Citations

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Research works printed but not linked: 1

  1. Turk, 1999, Autoantibodies against advanced glycation endproducts (AGEs) in IDDM, Diabetes, 48, A431

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Metadata

Authors
Turk, Z., Ljubic, S., Turk, N., & Benko, B.
Affiliations
  • University of Zagreb (HR)
  • Institute of Immunology (HR)
Corresponding author
Turk, Zdenka
ORCID
Turk, Nikša
Venue
Clinica Chimica Acta
Publisher
Elsevier BV
Published
2001-01
Volume
303
Issue
1-2
Pages
105-115
ISSN
0009-8981 (ISSN-L)
DOI
10.1016/s0009-8981(00)00389-2
OpenAlex
W2091966929
Cited by
111
References
21
Citations by year
2026: 6 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 5 · 2016: 6 · 2015: 7 · 2014: 5 · 2013: 4 · 2012: 11
FWCI
2.55
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Clinica Chimica Acta (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
  • Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
  • Health Sciences › Medicine › Dermatology › Skin Diseases and Diabetes
Keywords
advanced glycation end products, autoantibodies, diabetes mellitus, immune complex formation, atherosclerosis, polyethylene glycol precipitation
MeSH terms
Antigen-Antibody Complex, Autoantibodies, Diabetes Mellitus, Type 2, Enzyme-Linked Immunosorbent Assay, Humans, Glycation End Products, Advanced

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