Effects of ketamine on synaptic transmission and long-term potentiation in layer II/III of rat visual cortex in vitro
Abstract
The effects of ketamine, which has NMDA receptor antagonist properties, on synaptic transmission and long-term potentiation in layer II/III of adult rat visual cortex were examined in vitro. Field potentials were recorded in layer II/III following layer IV stimulation. Primed-burst stimulation was used for induction of long-term potentiation. Stimulation of layer IV resulted in a two-component response in layer II/III, a population excitatory postsynaptic potential1 (EPSP1) and a population excitatory postsynaptic potential2 (EPSP2). DL-2-Amino-5-phosphono-valeric acid (AP5), a competitive NMDA receptor antagonist, reduced the amplitude of the population EPSP1 while ketamine increased the amplitude of the population EPSP2. The results showed that primed-burst stimulation induced long-term potentiation in layer II/III of the visual cortex in vitro. Preincubation for 30 min with AP5 (25-100 microM) reduced the extent of long-term potentiation of the population EPSP2 and blocked the induction of long-term potentiation of the population EPSP1. When ketamine (100-200 microM) was present for 30 min prior to tetanic stimulation, it blocked the induction of long-term potentiation of the population EPSP1 and reduced the extent of long-term potentiation of the population EPSP2. We conclude that ketamine can interfere with synaptic transmission in the visual cortex. Primed-burst stimulation is an effective protocol for neocortical potentiation. NMDA receptors are involved in the induction of long-term potentiation by primed-burst stimulation of the population EPSP1 and population EPSP2 in adult rat visual cortex in vitro.
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- Authors
- Salami, M., Fathollahi, Y., Esteky, H., Motamedi, F., & Atapour, N.
- Affiliations
- Shahid Beheshti University (IR)
- Tarbiat Modares University (IR)
- Kerman University of Medical Sciences (IR)
- Corresponding author
- Salami, Mahmoud
- Venue
- European Journal of Pharmacology
- Publisher
- Elsevier BV
- Published
- 2000-03
- Volume
- 390
- Issue
- 3
- Pages
- 287-293
- ISSN
- 0014-2999 (ISSN-L)
- OpenAlex
- W2081753740
- Cited by
- 15
- References
- 29
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2016: 2 · 2015: 2 · 2014: 1
- FWCI
- 0.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
- Life Sciences › Neuroscience › Developmental Neuroscience › Anesthesia and Neurotoxicity Research
- Keywords
- ketamine, NMDA receptor antagonism, long-term potentiation, synaptic transmission, visual cortex, layer 2/3, excitatory postsynaptic potentials, AP5, tetanic stimulation
- MeSH terms
- Animals, Calcium, Electric Stimulation, Female, Ketamine, Male, Synaptic Transmission, Visual Cortex, 2-Amino-5-phosphonovalerate, Long-Term Potentiation, Excitatory Amino Acid Antagonists, Excitatory Postsynaptic Potentials, Rats, In Vitro Techniques
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