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Cited by 307Microtubule and mitosis dynamicsCellular transport and secretionGenetic and Kidney Cyst Diseases

Microtubules and the organization of the Golgi complex

Thyberg, Johan, Moskalewski, Stanislaw·1985·Experimental Cell Research
DOI 10.1016/s0014-4827(85)80032-x
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Abstract

Electron microscopic and cytochemical studies indicate that microtubules play an important role in the organization of the Golgi complex in mammalian cells. During interphase microtubules form a radiating pattern in the cytoplasm, originating from the pericentriolar region (microtubule-organizing centre). The stacks of Golgi cisternae and the associated secretory vesicles and lysosomes are arranged in a circumscribed juxtanuclear area, usually centered around the centrioles, and show a defined orientation in relation to the rough endoplasmic reticulum. Exposure of cells to drugs such as colchicine, vinblastine and nocodazole leads to disassembly of microtubules and disorganization of the Golgi complex, most typically a dispersion of its stacks of cisternae throughout the cytoplasm. These alterations are accompanied by disturbances in the intracellular transport, processing and release of secretory products as well as inhibition of endocytosis. The observations suggest that microtubules are partly responsible for the maintenance and functioning of the Golgi complex, possibly by arranging its stacks of cisternae three-dimensionally within the cell and in relation to other organelles and ensuring a normal flow of material into and away from them. During mitosis, microtubules disassemble (prophase) and a mitotic spindle is built up (metaphase) to take care of the subsequent separation of the chromosomes (anaphase). The breaking up of the microtubular cytoskeleton is followed by vesiculation of the rough endoplasmic reticulum and partial atrophy, as well as dispersion of the stacks of Golgi cisternae. After completion of the nuclear division (telophase), the radiating microtubule pattern is re-established and the rough endoplasmic reticulum and the Golgi complex resume their normal interphase structure. This sequence of events is believed to fulfil the double function to provide tubulin units and space for construction of the mitotic spindle and to guarantee an approximately equal distribution of the rough endoplasmic reticulum and the Golgi complex on the two daughter cells.

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  • Upon nocodazole addition, microtubules depolymerize and the Golgi is detected as characteristic mini-stacks that distribute throughout the cytoplasm.

    “Upon nocodazole addition, microtubules begin to depolymerize and the Golgi is detected as characteristic mini-stacks that distribute throughout the cytoplasm (Thyberg and Moskalewski, 1985).”

    From The Rab6-regulated KIF1C kinesin motor domain contributes to Golgi organization (2015) · cites this paper as Thyberg and Moskalewski, 1985

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Authors
Thyberg, J., & Moskalewski, S.
Affiliations
Karolinska Institutet (SE)
Corresponding author
Thyberg, Johan
Venue
Experimental Cell Research
Publisher
Elsevier BV
Published
1985-07
Volume
159
Issue
1
Pages
1-16
ISSN
0014-4827 (ISSN-L)
DOI
10.1016/s0014-4827(85)80032-x
OpenAlex
W1978864905
Cited by
307
References
118
Citations by year
2026: 2 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 7 · 2015: 7 · 2014: 5 · 2013: 4 · 2012: 3
FWCI
0.80
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Experimental Cell Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
microtubules, Golgi apparatus, Golgi cisternae, microtubule organizing center, centrioles, rough endoplasmic reticulum, secretory vesicles, lysosomes, intracellular transport, mitosis, mitotic spindle, microtubule disassembly, colchicine, vinblastine, nocodazole, cell division
MeSH terms
Insulin Secretion, Animals, Chick Embryo, Colchicine, Connective Tissue, Endocrine Glands, Endocytosis, Exocrine Glands, Exocytosis, Golgi Apparatus, Histocytochemistry, Humans, Insulin, Microscopy, Electron, Microtubules, Mitosis, Neurons

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