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Cited by 84Microtubule and mitosis dynamicsCellular transport and secretionGenetic and Kidney Cyst Diseases

Association of kinesin with the Golgi apparatus in rat hepatocytes

Marks, David L., Larkin, Janet M., McNiven, Mark A.·1994·Journal of Cell Science
DOI 10.1242/jcs.107.9.2417
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Abstract

The Golgi apparatus is a dynamic membranous structure, which has been observed to alter its location and morphology during the cell cycle and after microtubule disruption. These dynamics are believed to be supported by a close structural interaction of the Golgi with the microtubule cytoskeleton and associated motor enzymes. One microtubule-dependent motor enzyme, kinesin, has been implicated in Golgi movement and function although direct evidence supporting this interaction is lacking. In this study, we utilized two well-characterized kinesin antibodies in conjunction with subcellular fractionation techniques, immunoblot analysis and immunofluorescence microscopy to conduct a detailed study on the association of kinesin with the Golgi and other membranous organelles in a polarized epithelial cell, the primary rat hepatocyte. We found that kinesin represents approximately 0.3% of total protein in rat liver homogenates, with approximately 30% membrane-associated and the remainder in the cytosol. Among membrane fractions, kinesin was concentrated markedly in Golgi-enriched fractions, which were prepared using two independent techniques. Kinesin was also abundant in fractions enriched in transcytotic carriers and secretory vesicles, with lower levels detected on fractions enriched in endosomes, endoplasmic reticulum, lysosomes and mitochondria. Immunofluorescence microscopy showed that kinesin is concentrated on Golgi-like structures in both primary cultured hepatocytes and rat hepatocyte-derived clone 9 cells. Double-label immunofluorescence demonstrated that kinesin staining colocalizes with the Golgi marker, alpha-mannosidase II, in both cell types. These results provide compelling evidence showing that kinesin is associated with the Golgi complex in cells and implicate this motor enzyme in Golgi structure, function and dynamics.

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Crossref · 10.1242/jcs.107.9.2417

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  • The observation that faint but detectable KIF5A is seen in or near the Golgi apparatus in wild-type neurons, coupled to many previous observations of kinesin-I in association with Golgi elements in a variety of cell types, suggests that the Golgi region is a normal site of KIF5A binding.

    “However, the observation that faint but detectable KIF5A is seen in or near the Golgi apparatus in wild-type neurons, coupled to many previous observations of kinesin-I in association with Golgi elements in a variety of cell types (Marks et al. 1994; Lippincott-Schwartz et al. 1995; Johnson et al. 1996), suggests that the Golgi region is a normal site of KIF5A binding.”

    From Defective kinesin heavy chain behavior in mouse kinesin light chain mutants (1999) · cites this paper as Marks et al. 1994

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Authors
Marks, D. L., Larkin, J. M., & McNiven, M. A.
Affiliations
Mayo Clinic (US)
ORCID
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
1994-09-01
Volume
107
Issue
9
Pages
2417-2426
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.107.9.2417
OpenAlex
W2111028666
Cited by
84
References
64
Citations by year
2026: 1 · 2025: 1 · 2022: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2016: 3 · 2015: 1
FWCI
3.31
Type
journal article
Language
en
Open access
closed
Licence
www.biologists.com/user-licence-1-1/ (vor)
Locations
  • Journal of Cell Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01aa009227-07)
  • National Institute on Alcohol Abuse and Alcoholism (AA09227-02)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK44650)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
kinesin, Golgi apparatus, hepatocytes, Golgi dynamics, microtubule motor proteins, secretory vesicles, alpha-mannosidase II
MeSH terms
Amino Acid Sequence, Animals, Cells, Cultured, Fluorescent Antibody Technique, Golgi Apparatus, Liver, Microtubules, Molecular Sequence Data, Organ Specificity, Organelles, Kinesins, Rats

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